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Literature summary extracted from

  • Shin, J.H.; Park, S.H.; Oh, Y.H.; Choi, J.W.; Lee, M.H.; Cho, J.S.; Jeong, K.J.; Joo, J.C.; Yu, J.; Park, S.J.; Lee, S.Y.
    Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid (2016), Microb. Cell Fact., 15, 174 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.2 gene davB, recombinant expression in Escherichia coli, coexpression with gene davA, encoding 5-aminovaleramidase, recombinant expression of gene davB as N-terminally His-tagged enzyme in Corynebacterium glutamicum, a highly L-lysine producing strain BE using the sod promoter and the transcription factor Tu, EC 3.6.5.3 Pseudomonas putida
3.5.1.30 gene davA, recombinant expression in Corynebacterium glutamicum, coexpression with gene davB from Pseudomonas putida encoding lysine 2-monooxygenase (EC 1.13.12.2) Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.2 additional information engineering of a recombinant Escherichia coli strain expressing the davB and davA genes for bioconversion of L-lysine to 5-aminovaleric acid resulting in low levels of 5-aminovalerate. Development of metabolically engineered Corynebacterium glutamicum strains for enhanced fermentative production of 5-aminovalerate from glucose. Expression of the Corynebacterium glutamicum codon-optimized davA gene fused with His6-Tag at its N-terminus and the davB gene as an operon under a strong synthetic H36 promoter (plasmid p36davAB3) in Corynebacterium glutamicum strain BE (pJS38) enables the most efficient production of 5-aminovalerate. The construct containing the His6-tagged variant produces substantially more 5-aminovalerate compared to that produced using the construct lacking the His-tag, possibly because of the improved stability afforded by the 5'-modification, which results in higher expression of the davAB genes in the recombinant Corynebacterium glutamicum BE strain. Deletion of the gabT gene (EC 2.6.1.19), encoding 4-aminobutyrate aminotransferase, improves the 5-aminovalerate production Pseudomonas putida
3.5.1.30 additional information combined expression of Pseudomonas putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively, in engineered Corynebacterium glutamicum strain BE (KCTC 12390BP) for large scale production of 5-aminovalerate (5AVA) under the control of constitutive promoters. Expression of codon-optimized N-terminally His6-tagged davA gene fused the davB gene as an operon under a strong synthetic H36 promoter from plasmid p36davAB3 in Corynebacterium glutamicum in fed-batch culture enables the most efficient production of 5AVA. In addition, production of glutaric acid, a major byproduct, is significantly reduced by employing Corynebacterium glutamicum gabT mutant as a host. Method development and optimization, overview Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.2 L-lysine + O2 Pseudomonas putida
-
 5-aminopentanamide + CO2 + H2O
-
 ?
1.13.12.2 L-lysine + O2 Pseudomonas putida ATCC 12633
-
 5-aminopentanamide + CO2 + H2O
-
 ?
3.5.1.30 5-aminopentanamide + H2O Pseudomonas putida
-
 5-aminopentanoate + NH3
-
 ?
3.5.1.30 5-aminopentanamide + H2O Pseudomonas putida ATCC 12633
-
 5-aminopentanoate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.2 Pseudomonas putida
-
-
-
1.13.12.2 Pseudomonas putida ATCC 12633
-
-
-
3.5.1.30 Pseudomonas putida B3IVI7
-
-
3.5.1.30 Pseudomonas putida ATCC 12633 B3IVI7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.2 L-lysine + O2
-
 Pseudomonas putida 5-aminopentanamide + CO2 + H2O
-
 ?
1.13.12.2 L-lysine + O2
-
 Pseudomonas putida ATCC 12633 5-aminopentanamide + CO2 + H2O
-
 ?
3.5.1.30 5-aminopentanamide + H2O
-
 Pseudomonas putida 5-aminopentanoate + NH3
-
 ?
3.5.1.30 5-aminopentanamide + H2O
-
 Pseudomonas putida ATCC 12633 5-aminopentanoate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.2 davB
-
 Pseudomonas putida
1.13.12.2 L-lysine 2-monooxygenase
-
 Pseudomonas putida
3.5.1.30 5-aminovaleramidase
-
 Pseudomonas putida
3.5.1.30 DavA
-
 Pseudomonas putida
3.5.1.30 delta-aminovaleramidase
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.13.12.2 metabolism the enzyme is involved in the aminovalerate pathway, overview Pseudomonas putida