Literature summary extracted from

Schmitz, D.; Zapp, J.; Bernhardt, R.
Steroid conversion with CYP106A2 - production of pharmaceutically interesting DHEA metabolites (2014), Microb. Cell Fact., 13, 81 .

Application

EC Number	Application	Comment	Organism
1.14.15.8	synthesis	an overexpressing Bacillus megaterium strain produces up to 115 mg/l/h 7beta-hydroxydehydroepiandrosterone in whole-cell conversions	Priestia megaterium

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.15.8	exression in Bacillus megaterium	Priestia megaterium

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.15.8	Priestia megaterium	Q06069	isoform Cyp106A2	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.15.8	dehydroepiandrosterone + reduced adrenodoxin + O2	-	Priestia megaterium	7beta-hydroxy-dehydroepiandrosterone + oxidized adrenodoxin + H2O	-	?
1.14.15.8	dexamethasone + reduced adrenodoxin + O2	-	Priestia megaterium	? + oxidized adrenodoxin + H2O	-	?
1.14.15.8	digitoxigenin + reduced adrenodoxin + O2	-	Priestia megaterium	? + oxidized adrenodoxin + H2O	-	?
1.14.15.8	additional information	the 3-oxo-DELTA4 moiety is not crucial for steroid conversion with CYP106A2. No substrates: estrone, estradiol, stigmasterol, and cholesterol	Priestia megaterium	?	-	?
1.14.15.8	prednisone + reduced adrenodoxin + O2	-	Priestia megaterium	? + oxidized adrenodoxin + H2O	-	?
1.14.15.8	pregnenolone + reduced adrenodoxin + O2	-	Priestia megaterium	7beta-hydroxy-pregnenolone + oxidized adrenodoxin + H2O	-	?

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Release 2023.1 (January 2023)