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Literature summary extracted from

  • Khumkarjorn, N.; Thanonkeo, S.; Yamada, M.; Thanonkeo, P.
    Cloning and expression analysis of a flavanone 3-hydroxylase gene in Ascocenda orchid (2017), J. Plant Biochem. Biotechnol., 26, 179-190 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.9 ascorbate required x Ascocenda

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.9 gene F3H cloned from petals, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21 x Ascocenda

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.9 0.207
-
 naringenin pH 8.0, 45°C x Ascocenda

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.9 Fe2+ required for catalytic activity x Ascocenda

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2 x Ascocenda
-
 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2 x Ascocenda NK-2012
-
 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
1.14.11.9 naringenin + 2-oxoglutarate + O2 x Ascocenda
-
 dihydrokaempferol + succinate + CO2
-
 ?
1.14.11.9 naringenin + 2-oxoglutarate + O2 x Ascocenda NK-2012
-
 dihydrokaempferol + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 x Ascocenda J9QYL0 var. Subun
-
1.14.11.9 x Ascocenda NK-2012 J9QYL0 var. Subun
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.9 flower enzyme expression analysis of AcF3H at different developmental stages and in different parts of the flower, overview. The highest enzyme expression occurs when the petals turned pale-orange x Ascocenda
-
1.14.11.9 petal
-
 x Ascocenda
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
 x Ascocenda a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
 x Ascocenda NK-2012 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
1.14.11.9 naringenin + 2-oxoglutarate + O2
-
 x Ascocenda dihydrokaempferol + succinate + CO2
-
 ?
1.14.11.9 naringenin + 2-oxoglutarate + O2
-
 x Ascocenda NK-2012 dihydrokaempferol + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.9 ? x * 41220, sequence calculation, x * 41500, recombinant enzyme, SDS-PAGE x Ascocenda

Synonyms

EC Number Synonyms Comment Organism
1.14.11.9 AcF3H
-
 x Ascocenda
1.14.11.9 F3H
-
 x Ascocenda
1.14.11.9 flavanone 3-hydroxylase
-
 x Ascocenda

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.9 35
-
 recombinant enzyme x Ascocenda

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.11.9 20 50 recombinant enzyme, stable at 20-45°C, loss of activity above 45°C, inactivation at 60°C x Ascocenda

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.9 8
-
 recombinant enzyme x Ascocenda

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.11.9 x Ascocenda sequence calculation
-
 5.1