BRENDA - Enzyme Database

Functional characterization of flavanone 3-hydroxylase gene from Phyllanthus emblica (L.)

Kumar, A.; Singh, B.; Singh, K.; J. Plant Biochem. Biotechnol. 24, 453-460 (2015)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.11.9
ascorbate
required for activity
Phyllanthus emblica
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.11.9
gene PeF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli tsrain M15 pREP-4
Phyllanthus emblica
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.9
Fe2+
required for activity
Phyllanthus emblica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Phyllanthus emblica
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
Phyllanthus emblica
-
dihydroquercetin + succinate + CO2
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
Phyllanthus emblica
-
dihydrokaempferol + succinate + CO2
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.9
Phyllanthus emblica
T1UPN7
ten year old healthy tree growing in the botanical garden of Panjab University, Chandigarh, India under natural conditions
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
flower
-
Phyllanthus emblica
-
1.14.11.9
fruit
-
Phyllanthus emblica
-
1.14.11.9
leaf
-
Phyllanthus emblica
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
dihydroquercetin + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
dihydrokaempferol + succinate + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.11.9
More
three-dimensional protein structure modeling, overview. F3H has a jelly roll in the enzyme core, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Position between 194 and 295 amino acids represent the 2-oxoglutarate-ferrous(II) oxygenase superfamily domain which plays key role in catalyzing a redox reaction
Phyllanthus emblica
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.11.9
F3H
-
Phyllanthus emblica
1.14.11.9
flavanone 3-hydroxylase
-
Phyllanthus emblica
1.14.11.9
PeF3H
-
Phyllanthus emblica
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.11.9
37
-
assay at
Phyllanthus emblica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.11.9
7
-
assay at
Phyllanthus emblica
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.11.9
ascorbate
required for activity
Phyllanthus emblica
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.9
gene PeF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli tsrain M15 pREP-4
Phyllanthus emblica
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.9
Fe2+
required for activity
Phyllanthus emblica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Phyllanthus emblica
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
Phyllanthus emblica
-
dihydroquercetin + succinate + CO2
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
Phyllanthus emblica
-
dihydrokaempferol + succinate + CO2
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
flower
-
Phyllanthus emblica
-
1.14.11.9
fruit
-
Phyllanthus emblica
-
1.14.11.9
leaf
-
Phyllanthus emblica
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
dihydroquercetin + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
dihydrokaempferol + succinate + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.11.9
More
three-dimensional protein structure modeling, overview. F3H has a jelly roll in the enzyme core, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Position between 194 and 295 amino acids represent the 2-oxoglutarate-ferrous(II) oxygenase superfamily domain which plays key role in catalyzing a redox reaction
Phyllanthus emblica
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.11.9
37
-
assay at
Phyllanthus emblica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.11.9
7
-
assay at
Phyllanthus emblica
General Information
EC Number
General Information
Commentary
Organism
1.14.11.9
evolution
the enzyme belongs to the Fe(II)- and 2-oxoglutarate-dependent dioxygenase (2-ODD) superfamily, sharing the conserved motif of pfam 03171. F3H has a jelly roll in the enzyme core, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Phylogenetic and molecular evolutionary analyses
Phyllanthus emblica
1.14.11.9
additional information
three-dimensional protein structure modeling, overview
Phyllanthus emblica
1.14.11.9
physiological function
flavanone 3-hydroxylase (F3H) is an important regulatory enzyme of the flavonoid pathway which catalyzes the stereospecific hydroxylation of (2S)-naringenin to form (2R,3R)-dihydroflavonol. These dihydroflavonols serve as intermediates for the biosynthesis of anthocyanidins and flavonols
Phyllanthus emblica
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.11.9
evolution
the enzyme belongs to the Fe(II)- and 2-oxoglutarate-dependent dioxygenase (2-ODD) superfamily, sharing the conserved motif of pfam 03171. F3H has a jelly roll in the enzyme core, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Phylogenetic and molecular evolutionary analyses
Phyllanthus emblica
1.14.11.9
additional information
three-dimensional protein structure modeling, overview
Phyllanthus emblica
1.14.11.9
physiological function
flavanone 3-hydroxylase (F3H) is an important regulatory enzyme of the flavonoid pathway which catalyzes the stereospecific hydroxylation of (2S)-naringenin to form (2R,3R)-dihydroflavonol. These dihydroflavonols serve as intermediates for the biosynthesis of anthocyanidins and flavonols
Phyllanthus emblica