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Literature summary extracted from

  • Kalimuthu, P.; Heider, J.; Knack, D.; Bernhardt, P.V.
    Electrocatalytic hydrocarbon hydroxylation by ethylbenzene dehydrogenase from Aromatoleum aromaticum (2015), J. Phys. Chem. B, 119, 3456-3463 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.99.2 0.00045
-
 ethylbenzene at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 0.00186
-
 3-ethylpyridine at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 0.00232
-
 2-ethylpyrrole at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 0.00518
-
 2-Ethylphenol at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 0.00927
-
 3-Ethylphenol at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 0.0243
-
 2-ethylaniline at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 0.02469
-
 2-ethylfuran at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 0.04047
-
 4-Ethylphenol at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 0.06646
-
 2-ethylthiophene at pH 7.5 and 30°C Aromatoleum aromaticum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.99.2 Molybdenum
-
 Aromatoleum aromaticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.99.2 155000
-
 gel filtration Aromatoleum aromaticum

Organism

EC Number Organism UniProt Comment Textmining
1.17.99.2 Aromatoleum aromaticum
-
-
-
1.17.99.2 Aromatoleum aromaticum EbN1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.99.2 1,4-diethylbenzene + H2O + ferricenium 35% activity compared to ethylbenzene Aromatoleum aromaticum 1-(4-ethylphenyl)ethanol + 1,1'-(1,4-phenylene)diethanol + ferrocene
-
 ?
1.17.99.2 1,4-diethylbenzene + H2O + ferricenium 35% activity compared to ethylbenzene Aromatoleum aromaticum EbN1 1-(4-ethylphenyl)ethanol + 1,1'-(1,4-phenylene)diethanol + ferrocene
-
 ?
1.17.99.2 2-ethylaniline + H2O + ferricenium 92% activity compared to ethylbenzene Aromatoleum aromaticum ? + ferrocene
-
 ?
1.17.99.2 2-ethylaniline + H2O + ferricenium 92% activity compared to ethylbenzene Aromatoleum aromaticum EbN1 ? + ferrocene
-
 ?
1.17.99.2 2-ethylfuran + H2O + ferricenium 130% activity compared to ethylbenzene Aromatoleum aromaticum 1-(2-furyl)ethanol + ferrocene
-
 ?
1.17.99.2 2-ethylfuran + H2O + ferricenium 130% activity compared to ethylbenzene Aromatoleum aromaticum EbN1 1-(2-furyl)ethanol + ferrocene
-
 ?
1.17.99.2 2-ethylnaphthalene + H2O + ferricenium 9.3% activity compared to ethylbenzene Aromatoleum aromaticum ? + ferrocene
-
 ?
1.17.99.2 2-ethylphenol + H2O + ferricenium 55% activity compared to ethylbenzene Aromatoleum aromaticum 2-(1-hydroxyethyl)phenol + ferrocene
-
 ?
1.17.99.2 2-ethylpyrrole + H2O + ferricenium 228% activity compared to ethylbenzene Aromatoleum aromaticum ? + ferrocene
-
 ?
1.17.99.2 2-ethylthiophene + H2O + ferricenium 236% activity compared to ethylbenzene Aromatoleum aromaticum 1-thien-2-ylethanol + ferrocene
-
 ?
1.17.99.2 2-ethyltoluene + H2O + ferricenium 3.8% activity compared to ethylbenzene Aromatoleum aromaticum ? + ferrocene
-
 ?
1.17.99.2 3-ethylphenol + H2O + ferricenium 19% activity compared to ethylbenzene Aromatoleum aromaticum 3-(1-hydroxyethyl)phenol + ferrocene
-
 ?
1.17.99.2 3-ethylpyridine + H2O + ferricenium 16% activity compared to ethylbenzene Aromatoleum aromaticum ? + ferrocene
-
 ?
1.17.99.2 3-ethyltoluene + H2O + ferricenium 10% activity compared to ethylbenzene Aromatoleum aromaticum ? + ferrocene
-
 ?
1.17.99.2 4-ethylaniline + H2O + ferricenium 130% activity compared to ethylbenzene Aromatoleum aromaticum ? + ferrocene
-
 ?
1.17.99.2 4-ethylanisole + H2O + ferricenium 22% activity compared to ethylbenzene Aromatoleum aromaticum 1-(4-methoxyphenyl)ethanol + ferrocene
-
 ?
1.17.99.2 4-ethylbiphenyl + ferricenium + H2O
-
 Aromatoleum aromaticum 1-(1,1'-biphenyl-4-yl)ethanol + ferrocene
-
 ?
1.17.99.2 4-ethylbiphenyl + ferricenium + H2O
-
 Aromatoleum aromaticum EbN1 1-(1,1'-biphenyl-4-yl)ethanol + ferrocene
-
 ?
1.17.99.2 4-ethylbiphenyl + H2O + ferricenium 30% activity compared to ethylbenzene Aromatoleum aromaticum ? + ferrocene
-
 ?
1.17.99.2 4-ethylphenol + H2O + ferricenium 251% activity compared to ethylbenzene Aromatoleum aromaticum 4-(1-hydroxyethyl)phenol + ferrocene
-
 ?
1.17.99.2 4-ethyltoluene + H2O + ferricenium 28% activity compared to ethylbenzene Aromatoleum aromaticum 1-(4-methylphenyl)ethanol + ferrocene
-
 ?
1.17.99.2 4-fluoroethylbenzene + H2O + ferricenium 15% activity compared to ethylbenzene Aromatoleum aromaticum ? + ferrocene
-
 ?
1.17.99.2 4-propylphenol + H2O + ferricenium 175% activity compared to ethylbenzene Aromatoleum aromaticum 4-(1-hydroxypropyl)phenol + ferrocene
-
 ?
1.17.99.2 ethylbenzene + H2O + ferricenium 100% activity Aromatoleum aromaticum (S)-1-phenylethanol + ferrocene
-
 ?
1.17.99.2 additional information no activity with ethylidenecyclohexane, n-propylbenzene and 2-ethylpyrazine Aromatoleum aromaticum ?
-
 ?
1.17.99.2 additional information no activity with ethylidenecyclohexane, n-propylbenzene and 2-ethylpyrazine Aromatoleum aromaticum EbN1 ?
-
 ?
1.17.99.2 n-propylbenzene + H2O + ferricenium 14% activity compared to ethylbenzene Aromatoleum aromaticum 1-phenylpropanol + ferrocene
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.99.2 EBDH
-
 Aromatoleum aromaticum

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.99.2 bis(molybdopterin guanine dinucleotide)molybdenum
-
 Aromatoleum aromaticum
1.17.99.2 heme
-
 Aromatoleum aromaticum
1.17.99.2 iron-sulfur centre
-
 Aromatoleum aromaticum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.17.99.2 9.44
-
 2-ethylthiophene at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 10.05
-
 2-ethylaniline at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 14.03
-
 2-ethylfuran at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 16.53
-
 4-Ethylphenol at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 23.53
-
 3-ethylpyridine at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 24.62
-
 3-Ethylphenol at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 32.36
-
 2-Ethylphenol at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 261.62
-
 2-ethylpyrrole at pH 7.5 and 30°C Aromatoleum aromaticum
1.17.99.2 586.37
-
 ethylbenzene at pH 7.5 and 30°C Aromatoleum aromaticum