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Literature summary extracted from
- Characterization of ent-kaurene synthase and kaurene oxidase involved in gibberellin biosynthesis from Scoparia dulcis (2018), J. Nat. Med., 72, 456-463 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.86 | expression in Escherichia coli | Scoparia dulcis |
| 4.2.3.19 | expressed in Escherichia coli C41 (DE3) cells | Scoparia dulcis |
| 4.2.3.19 | gene KS, phylogenetic tree, quantitative real-time PCR expression analysis, functional recombinant expression in Escherichia coli strain C41(DE3), GC-MS analysis of diterpene products from hexane extracts of recombinant Escherichia coli harboring the pSdGGeC and pSdKS vectors | Scoparia dulcis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.19 | ent-copalyl diphosphate | Scoparia dulcis | - |
ent-kaurene + diphosphate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.86 | Scoparia dulcis | A0A0N7H9X2 | - |
- |
| 1.14.14.86 | Scoparia dulcis | A0A0N9YKX0 | - |
- |
| 4.2.3.19 | Scoparia dulcis | F6M9X0 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.86 | root | mainly expressed in the root and lateral root systems | Scoparia dulcis | - |
| 4.2.3.19 | leaf | - |
Scoparia dulcis | - |
| 4.2.3.19 | additional information | relative transcript levels of SdCPS1, SdKS, SdKO1, and SdKO2 are determined in young and mature leaves, upper and lower stems, roots, and lateral roots of Scoparia dulcis | Scoparia dulcis | - |
| 4.2.3.19 | root | - |
Scoparia dulcis | - |
| 4.2.3.19 | root | the SdKS gene is mainly expressed in the root and lateral root systems, which are elongating tissues, quantitative real-time PCR expression analysis | Scoparia dulcis | - |
| 4.2.3.19 | stem | - |
Scoparia dulcis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.86 | ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | - |
Scoparia dulcis | ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.86 | ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Scoparia dulcis | ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| 1.14.14.86 | ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Scoparia dulcis | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
| 1.14.14.86 | ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Scoparia dulcis | ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 4.2.3.19 | ent-copalyl diphosphate | - |
Scoparia dulcis | ent-kaurene + diphosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.86 | ? | x * 57900, calculated | Scoparia dulcis |
| 1.14.14.86 | ? | x * 58300, calculated | Scoparia dulcis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.86 | CYP701A50 | - |
Scoparia dulcis |
| 1.14.14.86 | CYP701A51 | - |
Scoparia dulcis |
| 1.14.14.86 | KO1 | - |
Scoparia dulcis |
| 1.14.14.86 | KO2 | - |
Scoparia dulcis |
| 4.2.3.19 | ent-KS | - |
Scoparia dulcis |
| 4.2.3.19 | SdKS | - |
Scoparia dulcis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.86 | cytochrome P450 | - |
Scoparia dulcis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.3.19 | evolution | the enzyme is a class I diterpene synthase | Scoparia dulcis |
| 4.2.3.19 | metabolism | gibberellins (GAs) are ubiquitous diterpenoids in higher plants, whereas some higher plants produce unique species-specific diterpenoids. In gibberellins biosynthesis, ent-kaurene synthase and ent-kaurene oxidase are key players which catalyze early step(s) of the cyclization and oxidation reactions | Scoparia dulcis |
| 4.2.3.19 | metabolism | the enzyme is involved in gibberellin biosynthesis | Scoparia dulcis |
| 4.2.3.19 | physiological function | enzyme SdKS catalyzes a cyclization reaction from ent-copalyl diphosphate to ent-kaurene and is a key enzyme in the gibberellins biosynthesis | Scoparia dulcis |
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