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Literature summary extracted from
- Purification of selenate reductase from Alcaligenes sp. CKCr-6A with the ability to biosynthesis of selenium nanoparticle Enzymatic behavior study in imidazolium based ionic liquids and organic solvent (2018), J. Mol. Liq., 249, 1254-1262 .
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.97.1.9 | Ag+ | 70% inhibition at 1 mM | Alcaligenes sp. |  |
| 1.97.1.9 | Ca2+ | about 20% inhibition at 1 mM | Alcaligenes sp. | |
| 1.97.1.9 | EDTA | inhibits the enzymatic reducing activity slightly, addition of Mo2+ and Zn2+ to the reaction mixture eliminates the EDTA inhibitory effect | Alcaligenes sp. | |
| 1.97.1.9 | Hg2+ | complete inhibition at 1 mM | Alcaligenes sp. | |
| 1.97.1.9 | Mn2+ | 35% inhibition at 1 mM | Alcaligenes sp. | |
| 1.97.1.9 | N-ethyl maleimide | complete inhibition | Alcaligenes sp. | |
| 1.97.1.9 | NaN3 | 60% inhibition at 1 mM | Alcaligenes sp. | |
| 1.97.1.9 | Pb2+ | 45% inhibition at 1 mM | Alcaligenes sp. | |
| 1.97.1.9 | Zn2+ | 30% inhibition at 1 mM | Alcaligenes sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.97.1.9 | 1.8 | - |
selenate | pH 6.0, 35°C | Alcaligenes sp. | |
| 1.97.1.9 | 5.47 | - |
Selenite | pH 6.0, 35°C | Alcaligenes sp. | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.97.1.9 | cytosol | - |
Alcaligenes sp. | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.97.1.9 | Mo2+ | role of Mo2+ in selenate reductase | Alcaligenes sp. | |
| 1.97.1.9 | additional information | Na+ and Mg2+ have no effect on the activity at 1 mM | Alcaligenes sp. |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.97.1.9 | 197000 | - |
gel filtration | Alcaligenes sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.97.1.9 | selenate + NADH + H+ | Alcaligenes sp. | - |
selenite + H2O + NAD+ | - |
? | |
| 1.97.1.9 | selenate + NADH + H+ | Alcaligenes sp. CKCr-6A | - |
selenite + H2O + NAD+ | - |
? | |
| 1.97.1.9 | selenite + NADH + H+ | Alcaligenes sp. | - |
selenium + H2O + NAD+ | - |
? | |
| 1.97.1.9 | selenite + NADH + H+ | Alcaligenes sp. CKCr-6A | - |
selenium + H2O + NAD+ | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.97.1.9 | Methanol | decreases enzyme activity but increases thermal inactivation of the enzyme | Alcaligenes sp. |
| 1.97.1.9 | n-propanol | decreases enzyme activity but increases thermal inactivation of the enzyme | Alcaligenes sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.97.1.9 | Alcaligenes sp. | - |
isolated from Sabzevar of Iran | - |
| 1.97.1.9 | Alcaligenes sp. CKCr-6A | - |
isolated from Sabzevar of Iran | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.97.1.9 | native enzyme 21.29fold by ammonium sulfate fractionation, anion exchange and hydrophonic interaction chromatography, followed by ultrafiltration | Alcaligenes sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.97.1.9 | 15.36 | - |
purified enzyme, pH 6.0, 35°C | Alcaligenes sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.97.1.9 | selenate + NADH + H+ | - |
Alcaligenes sp. | selenite + H2O + NAD+ | - |
? | |
| 1.97.1.9 | selenate + NADH + H+ | - |
Alcaligenes sp. CKCr-6A | selenite + H2O + NAD+ | - |
? | |
| 1.97.1.9 | selenite + NADH + H+ | - |
Alcaligenes sp. | selenium + H2O + NAD+ | - |
? | |
| 1.97.1.9 | selenite + NADH + H+ | - |
Alcaligenes sp. CKCr-6A | selenium + H2O + NAD+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.97.1.9 | heterotetramer | 1 * 70000 + 1 * 45000 + 1 * 35000 + 1 *27000, SDS-PAGE | Alcaligenes sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.97.1.9 | 35 | - |
- |
Alcaligenes sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.97.1.9 | 20 | 70 | activity range, profile overview, 70% and 35% of maximum activity at 50°C and 60°C | Alcaligenes sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.97.1.9 | additional information | - |
thermal stability analysis by differential scanning calorim | Alcaligenes sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.97.1.9 | 6 | - |
- |
Alcaligenes sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.97.1.9 | 5 | 9 | activity range, profile overview | Alcaligenes sp. |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.97.1.9 | physiological function | cell-free extracts of Alcaligenes sp. CKCr-6A catalyzes the NADH-dependent reduction of selenate and selenite to elemental selenium | Alcaligenes sp. |
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