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Literature summary extracted from

  • Gao, L.; Wang, M.; Chen, S.; Zhang, D.
    Biochemical characterization of a novel feruloyl esterase from Penicillium piceum and its application in biomass bioconversion (2016), J. Mol. Catal. B, 133, S388-S394 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.73 degradation low doses of enzyme (120 microg/g substrate) increases glucose yields released from corn stover, wheat bran, corn cob, and cassava stillage residues by 68.8%, 38.6%, 15.6%, and 20.0%, respectively Talaromyces piceae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Escherichia coli Talaromyces piceae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.73 molecular modeling of structure based on PDB entry 3WMT Talaromyces piceae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Talaromyces piceae A0A1S5XVA5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 22
-
 substrate methyl ferulate, pH 3.0, 70°C Talaromyces piceae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 methyl caffeate + H2O
-
 Talaromyces piceae caffeate + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Talaromyces piceae ferulate + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Talaromyces piceae p-coumarate + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Talaromyces piceae sinapinate + methanol
-
 ?
3.1.1.73 additional information enzyme is active in degradation of corn stover, wheat bran, corn cob, and cassava stillage Talaromyces piceae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 56300, calculated, x * 56000, SDS-PAGE Talaromyces piceae

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 carboxylic ester hydrolase
-
 Talaromyces piceae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 70
-
-
 Talaromyces piceae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 60
-
 65% of maximum activity Talaromyces piceae
3.1.1.73 80
-
 48% of maximum activity Talaromyces piceae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 60
-
 half-life 150 min Talaromyces piceae
3.1.1.73 70
-
 half-life 220 min Talaromyces piceae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 3
-
-
 Talaromyces piceae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 3 5 more than 96% of maximum activity Talaromyces piceae