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Literature summary extracted from
- Kinetic characteristics of purified tyrosinase from different species of Dioscorea (YAM) in aqueous and non-aqueous systems (2014), J. Mol. Catal. B, 108, 111-117 .
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | additional information | the enzymes is activated in the presence of 1-40% water-immiscible ether | Dioscorea cayenensis subsp. rotundata | |
| 1.14.18.1 | additional information | the enzymes is activated in the presence of 1-40% water-immiscible ether | Colocasia esculenta | |
| 1.14.18.1 | additional information | the enzymes is activated in the presence of 1-40% water-immiscible ether | Dioscorea praehensilis | |
| 1.14.18.1 | additional information | the enzymes is activated in the presence of 1-40% water-immiscible ether | Dioscorea alata |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | 2-mercaptoethanol | - |
Colocasia esculenta |  |
| 1.14.18.1 | 2-mercaptoethanol | - |
Dioscorea alata | |
| 1.14.18.1 | 2-mercaptoethanol | - |
Dioscorea cayenensis subsp. rotundata | |
| 1.14.18.1 | 2-mercaptoethanol | - |
Dioscorea praehensilis | |
| 1.14.18.1 | EDTA | - |
Colocasia esculenta | |
| 1.14.18.1 | EDTA | - |
Dioscorea alata | |
| 1.14.18.1 | EDTA | - |
Dioscorea cayenensis subsp. rotundata | |
| 1.14.18.1 | EDTA | - |
Dioscorea praehensilis | |
| 1.14.18.1 | glutathione | - |
Colocasia esculenta | |
| 1.14.18.1 | glutathione | - |
Dioscorea alata | |
| 1.14.18.1 | glutathione | - |
Dioscorea cayenensis subsp. rotundata | |
| 1.14.18.1 | glutathione | - |
Dioscorea praehensilis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | Cu2+ | a copper-containing enzyme | Dioscorea cayenensis subsp. rotundata | |
| 1.14.18.1 | Cu2+ | a copper-containing enzyme | Colocasia esculenta | |
| 1.14.18.1 | Cu2+ | a copper-containing enzyme | Dioscorea praehensilis | |
| 1.14.18.1 | Cu2+ | a copper-containing enzyme | Dioscorea alata | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 41300 | - |
gel filtration | Colocasia esculenta |
| 1.14.18.1 | 53400 | - |
gel filtration | Dioscorea cayenensis subsp. rotundata |
| 1.14.18.1 | 54800 | - |
gel filtration | Dioscorea alata |
| 1.14.18.1 | 57500 | - |
gel filtration | Dioscorea praehensilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 2 catechol + O2 | Dioscorea cayenensis subsp. rotundata | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | Colocasia esculenta | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | Dioscorea praehensilis | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | Dioscorea alata | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | Dioscorea cayenensis subsp. rotundata | - |
2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | Colocasia esculenta | - |
2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | Dioscorea praehensilis | - |
2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | Dioscorea alata | - |
2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | Dioscorea cayenensis subsp. rotundata | - |
? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | Colocasia esculenta | - |
? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | Dioscorea praehensilis | - |
? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | Dioscorea alata | - |
? | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | Dioscorea cayenensis subsp. rotundata | - |
dopaquinone + H2O | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | Colocasia esculenta | - |
dopaquinone + H2O | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | Dioscorea praehensilis | - |
dopaquinone + H2O | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | Dioscorea alata | - |
dopaquinone + H2O | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | Ethanol | 50% activity remains in 40% ethanol, protein precipitation at 70% | Colocasia esculenta |
| 1.14.18.1 | Ethanol | below 20% activity remains in 40% ethanol | Dioscorea cayenensis subsp. rotundata |
| 1.14.18.1 | Ethanol | below 20% activity remains in 40% ethanol | Dioscorea alata |
| 1.14.18.1 | Ethanol | below 20% activity remains in 40% ethanol, protein precipitation at 90% | Dioscorea praehensilis |
| 1.14.18.1 | ether | the enzymes is activated in the presence of 1-40% water-immiscible ether after 17 h, loss of 40% activity in 70/ ether after 17 h | Dioscorea cayenensis subsp. rotundata |
| 1.14.18.1 | ether | the enzymes is activated in the presence of 1-40% water-immiscible ether after 17 h, loss of 40% activity in 70/ ether after 17 h | Colocasia esculenta |
| 1.14.18.1 | ether | the enzymes is activated in the presence of 1-40% water-immiscible ether after 17 h, loss of 40% activity in 70/ ether after 17 h | Dioscorea praehensilis |
| 1.14.18.1 | ether | the enzymes is activated in the presence of 1-40% water-immiscible ether after 17 h, loss of 40% activity in 70/ ether after 17 h | Dioscorea alata |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Colocasia esculenta | - |
cultivars are grown around Ile-Ife, South Western Nigeria | - |
| 1.14.18.1 | Dioscorea alata | - |
cultivars are grown around Ile-Ife, South Western Nigeria | - |
| 1.14.18.1 | Dioscorea cayenensis subsp. rotundata | - |
cultivars are grown around Ile-Ife, South Western Nigeria | - |
| 1.14.18.1 | Dioscorea praehensilis | - |
cultivars are grown around Ile-Ife, South Western Nigeria | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | native enzyme 11.4fold by cation exchange chromatography, anion exchange chromatography, and gel filtration | Dioscorea cayenensis subsp. rotundata |
| 1.14.18.1 | native enzyme 13.0fold by cation exchange chromatography, anion exchange chromatography, and gel filtration | Dioscorea alata |
| 1.14.18.1 | native enzyme 4.5fold by cation exchange chromatography, anion exchange chromatography, and gel filtration | Dioscorea praehensilis |
| 1.14.18.1 | native enzyme 7.2fold by cation exchange chromatography, anion exchange chromatography, and gel filtration | Colocasia esculenta |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | tuber | - |
Dioscorea cayenensis subsp. rotundata | - |
| 1.14.18.1 | tuber | - |
Colocasia esculenta | - |
| 1.14.18.1 | tuber | - |
Dioscorea alata | - |
| 1.14.18.1 | tuber | high enzyme content | Dioscorea praehensilis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 530 | - |
purified enzyme, pH 6.5, 30°C | Colocasia esculenta |
| 1.14.18.1 | 746 | - |
purified enzyme, pH 6.5, 30°C | Dioscorea cayenensis subsp. rotundata |
| 1.14.18.1 | 1407 | - |
purified enzyme, pH 6.5, 30°C | Dioscorea alata |
| 1.14.18.1 | 1638 | - |
purified enzyme, pH 6.5, 30°C | Dioscorea praehensilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 2 catechol + O2 | - |
Dioscorea cayenensis subsp. rotundata | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | - |
Colocasia esculenta | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | - |
Dioscorea praehensilis | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | - |
Dioscorea alata | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | 51% activity compared to L-dopa | Colocasia esculenta | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | 85% activity compared to L-dopa | Dioscorea alata | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | 92% activity compared to L-dopa | Dioscorea cayenensis subsp. rotundata | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | 92% activity compared to L-dopa | Dioscorea praehensilis | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | - |
Dioscorea cayenensis subsp. rotundata | 2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | - |
Colocasia esculenta | 2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | - |
Dioscorea praehensilis | 2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | - |
Dioscorea alata | 2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | best substrate | Dioscorea cayenensis subsp. rotundata | 2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | best substrate | Colocasia esculenta | 2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | best substrate | Dioscorea praehensilis | 2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | best substrate | Dioscorea alata | 2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | - |
Dioscorea cayenensis subsp. rotundata | ? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | - |
Colocasia esculenta | ? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | - |
Dioscorea praehensilis | ? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | - |
Dioscorea alata | ? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | 22% activity compared to L-dopa | Colocasia esculenta | ? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | 23% activity compared to L-dopa | Dioscorea praehensilis | ? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | 27% activity compared to L-dopa | Dioscorea cayenensis subsp. rotundata | ? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | 28% activity compared to L-dopa | Dioscorea alata | ? | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | - |
Dioscorea cayenensis subsp. rotundata | dopaquinone + H2O | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | - |
Colocasia esculenta | dopaquinone + H2O | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | - |
Dioscorea praehensilis | dopaquinone + H2O | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | - |
Dioscorea alata | dopaquinone + H2O | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | 40% activity compared to L-dopa | Dioscorea alata | dopaquinone + H2O | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | 44% activity compared to L-dopa | Colocasia esculenta | dopaquinone + H2O | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | 53% activity compared to L-dopa | Dioscorea praehensilis | dopaquinone + H2O | - |
? | |
| 1.14.18.1 | DL-tyrosine + O2 | 57% activity compared to L-dopa | Dioscorea cayenensis subsp. rotundata | dopaquinone + H2O | - |
? | |
| 1.14.18.1 | additional information | no activity with resorcinol, phenol, and 2-naphthol | Dioscorea cayenensis subsp. rotundata | ? | - |
? | |
| 1.14.18.1 | additional information | no activity with resorcinol, phenol, and 2-naphthol | Colocasia esculenta | ? | - |
? | |
| 1.14.18.1 | additional information | no activity with resorcinol, phenol, and 2-naphthol | Dioscorea praehensilis | ? | - |
? | |
| 1.14.18.1 | additional information | no activity with resorcinol, phenol, and 2-naphthol | Dioscorea alata | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | dimer | 2 * 24500, SDS-PAGE | Colocasia esculenta |
| 1.14.18.1 | dimer | 2 * 25900, SDS-PAGE | Dioscorea alata |
| 1.14.18.1 | dimer | 2 * 26800, SDS-PAGE | Dioscorea cayenensis subsp. rotundata |
| 1.14.18.1 | dimer | 2 * 26800, SDS-PAGE | Dioscorea praehensilis |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.009 | - |
2-mercaptoethanol | pH 6.5, 30°C | Dioscorea cayenensis subsp. rotundata | |
| 1.14.18.1 | 0.01 | - |
2-mercaptoethanol | pH 6.5, 30°C | Colocasia esculenta | |
| 1.14.18.1 | 0.01 | - |
2-mercaptoethanol | pH 6.5, 30°C | Dioscorea praehensilis | |
| 1.14.18.1 | 0.01 | - |
2-mercaptoethanol | pH 6.5, 30°C | Dioscorea alata | |
| 1.14.18.1 | 0.03 | - |
glutathione | pH 6.5, 30°C | Dioscorea cayenensis subsp. rotundata | |
| 1.14.18.1 | 0.03 | - |
glutathione | pH 6.5, 30°C | Colocasia esculenta | |
| 1.14.18.1 | 0.03 | - |
glutathione | pH 6.5, 30°C | Dioscorea alata | |
| 1.14.18.1 | 0.035 | - |
glutathione | pH 6.5, 30°C | Dioscorea praehensilis | |
| 1.14.18.1 | 7.9 | - |
EDTA | pH 6.5, 30°C | Dioscorea alata | |
| 1.14.18.1 | 8.6 | - |
EDTA | pH 6.5, 30°C | Colocasia esculenta | |
| 1.14.18.1 | 9 | - |
EDTA | pH 6.5, 30°C | Dioscorea praehensilis | |
| 1.14.18.1 | 10.9 | - |
EDTA | pH 6.5, 30°C | Dioscorea cayenensis subsp. rotundata | |
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