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Literature summary extracted from

  • Nair, N.; Raff, H.; Islam, M.T.; Feen, M.; Garofalo, D.M.; Sheppard, K.
    The Bacillus subtilis and Bacillus halodurans aspartyl-tRNA synthetases retain recognition of tRNAAsn (2016), J. Mol. Biol., 428, 618-630 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.12 expressed in Escherichia coli JF448 cells Mycoplasma pneumoniae
6.1.1.23 expressed in Escherichia coli JF448 cells Borreliella burgdorferi
6.1.1.23 expressed in Escherichia coli JF448 cells Halalkalibacterium halodurans
6.1.1.23 expressed in Escherichia coli JF448 cells Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.23 0.0007
-
 tRNAAsn at pH 8.0 and 37°C Halalkalibacterium halodurans
6.1.1.23 0.0015
-
 tRNAAsp at pH 8.0 and 37°C Halalkalibacterium halodurans
6.1.1.23 0.0016
-
 tRNAAsp at pH 7.2 and 37°C Bacillus subtilis
6.1.1.23 0.0016
-
 tRNAAsn at pH 7.2 and 37°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp Mycoplasma pneumoniae
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp Borreliella burgdorferi
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp Halalkalibacterium halodurans
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp Bacillus subtilis
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp Bacillus subtilis 168
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.12 Mycoplasma pneumoniae
-
-
-
6.1.1.23 Bacillus subtilis O32038
-
-
6.1.1.23 Bacillus subtilis 168 O32038
-
-
6.1.1.23 Borreliella burgdorferi
-
-
-
6.1.1.23 Halalkalibacterium halodurans Q9KDG1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Mycoplasma pneumoniae AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp 100% activity Mycoplasma pneumoniae AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 additional information the enzyme shows no activity with tRNAAsn and tRNAGln Mycoplasma pneumoniae ?
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn about 20% activity compared to tRNAAsp Halalkalibacterium halodurans AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn about 20% activity compared to tRNAAsp Bacillus subtilis AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn about 60% activity compared to tRNAAsp Borreliella burgdorferi AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn about 20% activity compared to tRNAAsp Bacillus subtilis 168 AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
 Borreliella burgdorferi AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
 Halalkalibacterium halodurans AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
 Bacillus subtilis AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp 100% activity Borreliella burgdorferi AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp 100% activity Halalkalibacterium halodurans AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp 100% activity Bacillus subtilis AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
 Bacillus subtilis 168 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp 100% activity Bacillus subtilis 168 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 additional information the enzyme shows no activity with tRNAGln Borreliella burgdorferi ?
-
 ?
6.1.1.23 additional information the enzyme shows no activity with tRNAGln Halalkalibacterium halodurans ?
-
 ?
6.1.1.23 additional information the enzyme shows no activity with tRNAGln Bacillus subtilis ?
-
 ?
6.1.1.23 additional information the enzyme shows no activity with tRNAGln Bacillus subtilis 168 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.12 Aspartyl-tRNA synthetase
-
 Mycoplasma pneumoniae
6.1.1.12 AspRS
-
 Mycoplasma pneumoniae
6.1.1.23 Aspartyl-tRNA synthetase
-
 Borreliella burgdorferi
6.1.1.23 Aspartyl-tRNA synthetase
-
 Halalkalibacterium halodurans
6.1.1.23 Aspartyl-tRNA synthetase
-
 Bacillus subtilis
6.1.1.23 AspRS
-
 Borreliella burgdorferi
6.1.1.23 AspRS
-
 Halalkalibacterium halodurans
6.1.1.23 AspRS
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.23 0.012
-
 tRNAAsn at pH 8.0 and 37°C Halalkalibacterium halodurans
6.1.1.23 0.058
-
 tRNAAsn at pH 7.2 and 37°C Bacillus subtilis
6.1.1.23 0.115
-
 tRNAAsp at pH 8.0 and 37°C Halalkalibacterium halodurans
6.1.1.23 0.36
-
 tRNAAsp at pH 7.2 and 37°C Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.12 ATP
-
 Mycoplasma pneumoniae
6.1.1.23 ATP
-
 Borreliella burgdorferi
6.1.1.23 ATP
-
 Halalkalibacterium halodurans
6.1.1.23 ATP
-
 Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.23 17
-
 tRNAAsn at pH 8.0 and 37°C Halalkalibacterium halodurans
6.1.1.23 40
-
 tRNAAsn at pH 7.2 and 37°C Bacillus subtilis
6.1.1.23 80
-
 tRNAAsp at pH 8.0 and 37°C Halalkalibacterium halodurans
6.1.1.23 220
-
 tRNAAsp at pH 7.2 and 37°C Bacillus subtilis