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Literature summary extracted from

  • Lee, J.P.; Kim, Y.A.; Kim, S.K.; Kim, H.
    Characterization of a multimodular endo-beta-1,4-glucanase (Cel9K) from Paenibacillus sp. X4 with a potential additive for saccharification (2018), J. Microbiol. Biotechnol., 28, 588-596 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 degradation addition of Cel9K to a commercial enzyme set (Celluclast 1.5L + Novozym 188) increases the saccharification of the pretreated reed and rice straw powders by 30.4% and 15.9%, respectively Paenibacillus sp. X4

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Paenibacillus sp. X4

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Co2+ 2 mM, less than 50% of initial activity Paenibacillus sp. X4
3.2.1.4 Mn2+ 2 mM, less than 50% of initial activity Paenibacillus sp. X4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ba2+ 2 mM, more than 150% of initial activity Paenibacillus sp. X4
3.2.1.4 K+ 2 mM, more than 150% of initial activity Paenibacillus sp. X4
3.2.1.4 Na+ 2 mM, more than 150% of initial activity Paenibacillus sp. X4

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Paenibacillus sp. X4 MG570051
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 barley beta-glucan + H2O 311% of the activity with carboxymethylcellulose Paenibacillus sp. X4 ?
-
?
3.2.1.4 carboxymethylcellulose + H2O
-
Paenibacillus sp. X4 ?
-
?
3.2.1.4 lichenan + H2O 157% of the activity with carboxymethylcellulose Paenibacillus sp. X4 ?
-
?
3.2.1.4 additional information low hydrolytic activities, toward birchwood xylan and laminarin Paenibacillus sp. X4 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 103500, calculated, x * 93000, SDS-PAGE, truncated form produced by the proteolytic cleavage of the C-terminus Paenibacillus sp. X4

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel9K
-
Paenibacillus sp. X4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
-
Paenibacillus sp. X4

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 40
-
56% of maximum activity Paenibacillus sp. X4
3.2.1.4 60
-
67% of maximum activity Paenibacillus sp. X4

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 50
-
half-life 59 min Paenibacillus sp. X4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.5
-
-
Paenibacillus sp. X4

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.4 Paenibacillus sp. X4 calculated
-
4.8