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Literature summary extracted from

  • Kogan, N.M.; Melamed, E.; Wasserman, E.; Raphael, B.; Breuer, A.; Stok, K.S.; Sondergaard, R.; Escudero, A.V.; Baraghithy, S.; Attar-Namdar, M.; Friedlander-Barenboim, S.; Mathavan, N.; Isaksson, H.; Mechoulam, R.; Mueller, R.; Bajayo, A.; Gabet, Y.; Bab, I.
    Cannabidiol, a major non-psychotropic cannabis constituent enhances fracture healing and stimulates lysyl hydroxylase activity in osteoblasts (2015), J. Bone Miner. Res., 30, 1905-1913 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.4 cannabidiol CBD, a major non-psychotropic cannabis constituent, stimulates lysyl hydroxylase 1 activity in osteoblasts, cannabidiol enhances the biomechanical properties of healing rat mid-femoral fractures Rattus norvegicus
1.14.11.4 DELTA9-tetrahydrocannabinol THC, the psychotropic cannabis constituent, stimulates lysyl hydroxylase 2 activity in osteoblasts Rattus norvegicus
1.14.11.4 additional information no stimulating effect of LH1 by DELTA9-tetrahydrocannabinol (THC), but THC potentiates the CBD stimulated work-to-failure at 6 weeks postfracture followed by attenuation of the CBD effect at 8 weeks Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.4 gene Plod1, real-time RT-PCR enzyme expression analysis Rattus norvegicus
1.14.11.4 gene Plod2, real-time RT-PCR enzyme expression analysis Rattus norvegicus
1.14.11.4 gene Plod3, real-time RT-PCR enzyme expression analysis Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.4 [procollagen]-L-lysine + 2-oxoglutarate + O2 Rattus norvegicus
-
 [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.4 Rattus norvegicus Q5U367
-
-
1.14.11.4 Rattus norvegicus Q63321
-
-
1.14.11.4 Rattus norvegicus Q811A3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.4 bone
-
 Rattus norvegicus
-
1.14.11.4 osteoblast
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.4 [procollagen]-L-lysine + 2-oxoglutarate + O2
-
 Rattus norvegicus [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.4 LH1
-
 Rattus norvegicus
1.14.11.4 LH2
-
 Rattus norvegicus
1.14.11.4 LH3
-
 Rattus norvegicus
1.14.11.4 Plod1
-
 Rattus norvegicus
1.14.11.4 PLOD2
-
 Rattus norvegicus
1.14.11.4 PLOD3
-
 Rattus norvegicus

Expression

EC Number Organism Comment Expression
1.14.11.4 Rattus norvegicus no induction of PLOD3 expression in osteoblasts by DELTA9-tetrahydrocannabinol and cannabidiol additional information
1.14.11.4 Rattus norvegicus cannabidiol stimulates mRNA expression of Plod1 in primary osteoblast cultures up
1.14.11.4 Rattus norvegicus DELTA9-tetrahydrocannabinol stimulates mRNA expression of Plod2 in primary osteoblast cultures up

General Information

EC Number General Information Comment Organism
1.14.11.4 physiological function the enzyme catalyzes lysine hydroxylation, which is involved in collagen crosslinking and stabilization. Critical mechanical role of collagen crosslinking enzymes, e.g. in bone healing. Lysyl hydroxylase 1 (PLOD1) is one of the few collagen crosslinking enzymes associated with bone quality Rattus norvegicus
1.14.11.4 physiological function the enzyme LH2 catalyzes lysine hydroxylation, which is involved in collagen crosslinking and stabilization. PLOD2 has no role in determining bone quality Rattus norvegicus
1.14.11.4 physiological function the enzyme LH3 catalyzes lysine hydroxylation Rattus norvegicus