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Literature summary extracted from

  • Gagoski, D.; Shi, Z.; Nielsen, L.K.; Vickers, C.E.; Mahler, S.; Speight, R.; Johnston, W.A.; Alexandrov, K.
    Cell-free pipeline for discovery of thermotolerant xylanases and endo-1,4-beta-glucanases (2017), J. Biotechnol., 259, 191-198 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 analysis construction of a pipeline based on Leishmania tarentolae cell-free system to characterize 30 putative thermostable endo-1,4-beta-glucanases and xylanases identified in public genomic databases. The system uses high-throughput assays for glucanase and xylanase activities that rely on solubilisation of labelled particulate substrates performed in multiwell plates Bacillus subtilis
3.2.1.4 analysis construction of a pipeline based on Leishmania tarentolae cell-free system to characterize 30 putative thermostable endo-1,4-beta-glucanases and xylanases identified in public genomic databases. The system uses high-throughput assays for glucanase and xylanase activities that rely on solubilisation of labelled particulate substrates performed in multiwell plates Castellaniella defragrans
3.2.1.4 analysis construction of a pipeline based on Leishmania tarentolae cell-free system to characterize 30 putative thermostable endo-1,4-beta-glucanases and xylanases identified in public genomic databases. The system uses high-throughput assays for glucanase and xylanase activities that rely on solubilisation of labelled particulate substrates performed in multiwell plates Halosimplex carlsbadense
3.2.1.8 analysis construction of a pipeline based on Leishmania tarentolae cell-free system to characterize 30 putative thermostable endo-1,4-beta-glucanases and xylanases identified in public genomic databases. The system uses high-throughput assays for glucanase and xylanase activities that rely on solubilisation of labelled particulate substrates performed in multiwell plates Bacillus subtilis
3.2.1.8 analysis construction of a pipeline based on Leishmania tarentolae cell-free system to characterize 30 putative thermostable endo-1,4-beta-glucanases and xylanases identified in public genomic databases. The system uses high-throughput assays for glucanase and xylanase activities that rely on solubilisation of labelled particulate substrates performed in multiwell plates Humicola insolens
3.2.1.8 analysis construction of a pipeline based on Leishmania tarentolae cell-free system to characterize 30 putative thermostable endo-1,4-beta-glucanases and xylanases identified in public genomic databases. The system uses high-throughput assays for glucanase and xylanase activities that rely on solubilisation of labelled particulate substrates performed in multiwell plates Thermothielavioides terrestris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Bacillus subtilis
3.2.1.4 expression in Escherichia coli Castellaniella defragrans
3.2.1.4 expression in Escherichia coli Halosimplex carlsbadense
3.2.1.8 expression in Escherichia coli Bacillus subtilis
3.2.1.8 expression in Escherichia coli Humicola insolens
3.2.1.8 expression in Escherichia coli Thermothielavioides terrestris

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Bacillus subtilis
-
-
-
3.2.1.4 Castellaniella defragrans
-
-
-
3.2.1.4 Halosimplex carlsbadense
-
-
-
3.2.1.8 Bacillus subtilis
-
-
-
3.2.1.8 Humicola insolens
-
-
-
3.2.1.8 Thermothielavioides terrestris
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 azurine-labelled hydroxyethylcellulose + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.4 azurine-labelled hydroxyethylcellulose + H2O
-
 Castellaniella defragrans ?
-
 ?
3.2.1.4 azurine-labelled hydroxyethylcellulose + H2O
-
 Halosimplex carlsbadense ?
-
 ?
3.2.1.8 azurin-labelled birchwood xylan + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.8 azurin-labelled birchwood xylan + H2O
-
 Humicola insolens ?
-
 ?
3.2.1.8 azurin-labelled birchwood xylan + H2O
-
 Thermothielavioides terrestris ?
-
 ?