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Literature summary extracted from

  • Carluccio, C.; Fraternali, F.; Salvatore, F.; Fornili, A.; Zagari, A.
    Towards the identification of the allosteric Phe-binding site in phenylalanine hydroxylase (2016), J. Biomol. Struct. Dyn., 34, 497-507 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.1 expressed in Escherichia coli BW25113DELTAtnaA cells Pseudomonas fluorescens
1.14.16.1 expressed in Escherichia coli BW25113DELTAtnaA cells Pseudomonas aeruginosa
1.14.16.1 expressed in Escherichia coli BW25113DELTAtnaA cells Pseudomonas putida
1.14.16.1 expressed in Escherichia coli BW25113DELTAtnaA cells Cupriavidus necator
1.14.16.1 expressed in Escherichia coli BW25113DELTAtnaA cells Xanthomonas campestris

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.1 G46S the mutant shows lower allosteric Phe-binding ability compared to the wild type enzyme Homo sapiens
1.14.16.1 W179/L98Y the mutant shows 46% activity with L-phenylalanine and 1207% activity with L-tryptophan compared to the wild type enzyme Xanthomonas campestris
1.14.16.1 W179F the mutant shows 80% activity with L-phenylalanine and 1739% activity with L-tryptophan compared to the wild type enzyme Xanthomonas campestris
1.14.16.1 W179F/L98Y/Y231C the mutant shows 17% activity with L-phenylalanine and 570% activity with L-tryptophan compared to the wild type enzyme Xanthomonas campestris
1.14.16.1 W179F/Y231C the mutant shows 52% activity with L-phenylalanine and 952% activity with L-tryptophan compared to the wild type enzyme Xanthomonas campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 Pseudomonas fluorescens
-
 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 Pseudomonas aeruginosa
-
 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 Pseudomonas putida
-
 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 Cupriavidus necator
-
 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 Xanthomonas campestris
-
 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 Homo sapiens
-
 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Cupriavidus necator
-
-
-
1.14.16.1 Homo sapiens P00439
-
-
1.14.16.1 Pseudomonas aeruginosa
-
-
-
1.14.16.1 Pseudomonas fluorescens
-
-
-
1.14.16.1 Pseudomonas putida
-
-
-
1.14.16.1 Xanthomonas campestris
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2
-
 Pseudomonas fluorescens L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2
-
 Pseudomonas aeruginosa L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2
-
 Pseudomonas putida L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2
-
 Cupriavidus necator L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2
-
 Xanthomonas campestris L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2
-
 Homo sapiens L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 preferred substrate Pseudomonas fluorescens L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 preferred substrate Pseudomonas aeruginosa L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 preferred substrate Pseudomonas putida L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 preferred substrate Cupriavidus necator L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 preferred substrate Xanthomonas campestris L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-tryptophan + tetrahydrobiopterin + O2
-
 Pseudomonas fluorescens 5-hydroxytryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-tryptophan + tetrahydrobiopterin + O2
-
 Pseudomonas aeruginosa 5-hydroxytryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-tryptophan + tetrahydrobiopterin + O2
-
 Pseudomonas putida 5-hydroxytryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-tryptophan + tetrahydrobiopterin + O2
-
 Cupriavidus necator 5-hydroxytryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-tryptophan + tetrahydrobiopterin + O2
-
 Xanthomonas campestris 5-hydroxytryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.1 homotetramer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.16.1 P4H
-
 Pseudomonas fluorescens
1.14.16.1 P4H
-
 Pseudomonas aeruginosa
1.14.16.1 P4H
-
 Pseudomonas putida
1.14.16.1 P4H
-
 Cupriavidus necator
1.14.16.1 P4H
-
 Xanthomonas campestris
1.14.16.1 PAH
-
 Homo sapiens
1.14.16.1 phenylalanine 4-hydroxylase
-
 Pseudomonas fluorescens
1.14.16.1 phenylalanine 4-hydroxylase
-
 Pseudomonas aeruginosa
1.14.16.1 phenylalanine 4-hydroxylase
-
 Pseudomonas putida
1.14.16.1 phenylalanine 4-hydroxylase
-
 Cupriavidus necator
1.14.16.1 phenylalanine 4-hydroxylase
-
 Xanthomonas campestris
1.14.16.1 phenylalanine hydroxylase
-
 Pseudomonas fluorescens
1.14.16.1 phenylalanine hydroxylase
-
 Pseudomonas aeruginosa
1.14.16.1 phenylalanine hydroxylase
-
 Pseudomonas putida
1.14.16.1 phenylalanine hydroxylase
-
 Cupriavidus necator
1.14.16.1 phenylalanine hydroxylase
-
 Xanthomonas campestris
1.14.16.1 phenylalanine hydroxylase
-
 Homo sapiens
1.14.16.1 phhA
-
 Pseudomonas fluorescens
1.14.16.1 phhA
-
 Pseudomonas aeruginosa
1.14.16.1 phhA
-
 Pseudomonas putida
1.14.16.1 phhA
-
 Cupriavidus necator
1.14.16.1 phhA
-
 Xanthomonas campestris