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Literature summary extracted from

  • Kracher, D.; Andlar, M.; Furtmueller, P.; Ludwig, R.
    Active-site copper reduction promotes substrate binding of fungal lytic polysaccharide monooxygenase and reduces stability (2018), J. Biol. Chem., 293, 1676-1687 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.54 expression in Pichia pastoris Neurospora crassa
1.14.99.56 expression in Pichia pastoris Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.54 Cu2+ the reduction of the mononuclear active-site copper by ascorbic acid increases the affinity and the maximum binding capacity of LPMO for cellulose Neurospora crassa
1.14.99.56 Cu2+ the reduction of the mononuclear active-site copper by ascorbic acid increases the affinity and the maximum binding capacity of LPMO for cellulose Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.54 Neurospora crassa
-
-
-
1.14.99.56 Neurospora crassa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.54 cellulose + ascorbic acid + O2
-
 Neurospora crassa ? + dehydroascorbic acid + H2O
-
 ?
1.14.99.54 soluble beta-glucan + ascorbic acid + O2
-
 Neurospora crassa ? + dehydroascorbic acid + H2O
-
 ?
1.14.99.56 cellulose + ascorbic acid + O2
-
 Neurospora crassa ? + dehydroascorbic acid + H2O
-
 ?
1.14.99.56 soluble beta-glucan + ascorbic acid + O2
-
 Neurospora crassa ? + dehydroascorbic acid + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.54 LPMO9C
-
 Neurospora crassa
1.14.99.56 LPMO9C
-
 Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.99.54 35
-
 midpoint transition temperature, pH 4.0, citrate buffer, oxidized enzyme Neurospora crassa
1.14.99.54 44
-
 midpoint transition temperature, pH 4.0, acetate buffer, oxidized enzyme Neurospora crassa
1.14.99.54 48.8
-
 midpoint transition temperature, pH 6.0, phosphate buffer, presence of ascorbic acid Neurospora crassa
1.14.99.54 53
-
 midpoint transition temperature, pH 6.0, phosphate buffer, presence of EDTA Neurospora crassa
1.14.99.54 61.5
-
 midpoint transition temperature, pH 6.0, phosphate buffer, oxidized enzyme Neurospora crassa
1.14.99.56 35
-
 midpoint transition temperature, pH 4.0, citrate buffer, oxidized enzyme Neurospora crassa
1.14.99.56 44
-
 midpoint transition temperature, pH 4.0, acetate buffer, oxidized enzyme Neurospora crassa
1.14.99.56 48.8
-
 midpoint transition temperature, pH 6.0, phosphate buffer, presence of ascorbic acid Neurospora crassa
1.14.99.56 53
-
 midpoint transition temperature, pH 6.0, phosphate buffer, presence of EDTA Neurospora crassa
1.14.99.56 61.5
-
 midpoint transition temperature, pH 6.0, phosphate buffer, oxidized enzyme Neurospora crassa

General Information

EC Number General Information Comment Organism
1.14.99.54 metabolism substrates cellulose and xyloglucan show a stabilizing effect on the apparent transition midpoint temperature of the reduced, catalytically active enzyme. Oxidative auto-inactivation and destabilization are observed in the absence of a suitable substrate Neurospora crassa
1.14.99.56 metabolism substrates cellulose and xyloglucan show a stabilizing effect on the apparent transition midpoint temperature of the reduced, catalytically active enzyme. Oxidative auto-inactivation and destabilization are observed in the absence of a suitable substrate Neurospora crassa