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Literature summary extracted from

  • Goedegebuur, F.; Dankmeyer, L.; Gualfetti, P.; Karkehabadi, S.; Hansson, H.; Jana, S.; Huynh, V.; Kelemen, B.R.; Kruithof, P.; Larenas, E.A.; Teunissen, P.J.M.; Stahlberg, J.; Payne, C.M.; Mitchinson, C.; Sandgren, M.
    Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution (2017), J. Biol. Chem., 292, 17418-17430 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 expressed in Aspergillus niger variant awamori AP4 Trichoderma reesei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.176 sitting drop vapor diffusion method, using 25.5% (w/v) PEG 4000, 0.17 M ammonium sulfate, and 15% (v/v) glycerol Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 G22D/N49S/A68T/P227L/S278P/T296P the mutant shows an increase in melting temperature and a greater half-life compared with the wild type enzyme Trichoderma reesei
3.2.1.176 S8P/G22D/T41I/N49S/A68T/S113N/P227L /D249K/S278P/T296P/N301R the mutant shows an increase in melting temperature and a greater half-life compared with the wild type enzyme Trichoderma reesei
3.2.1.176 S8P/T41I/N49S/A68T/N89D/S92T/S113N /S196T/P227L/D249K/T255P/S278P/E295K /T296P/T332Y/V403D/S411F/T462I the mutant, which is active up to 75°C, shows a 10.4°C increase in melting temperature and a 44fold greater half-life compared with the wild type enzyme Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.176 cellulose + H2O Trichoderma reesei
-
 cellobiose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Trichoderma reesei P62694
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.176 ammonium sulfate precipitation and phenyl Sepharose resin column chromatography Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-methylumbelliferyl beta-lactoside + H2O
-
 Trichoderma reesei 4-methylumbelliferol + beta-lactose
-
 ?
3.2.1.176 cellulose + H2O
-
 Trichoderma reesei cellobiose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel7A
-
 Trichoderma reesei
3.2.1.176 cellobiohydrolase
-
 Trichoderma reesei
3.2.1.176 cellobiohydrolase I
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.176 53
-
-
 Trichoderma reesei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.176 50 65 the activity is highest at 53°C and slightly lower at 65°C, whereas the enzyme is practically inactive at 75°C Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.176 62 69 the melting temperature is 62.5°C. The wild type enzyme shows half-lives of 19.9, 9.3 and 8.6 min at 62°C, 66°C, and 69°C, respectively Trichoderma reesei