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Literature summary extracted from
- Functional analysis of human cytochrome P450 21A2 variants involved in congenital adrenal hyperplasia (2017), J. Biol. Chem., 292, 10767-10778 .
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.14.14.16 | in omplex with 17alpha-hydroxyprogesterone, to 3.3 A resolution | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.14.16 | G292C | 0.32% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | G292S | 0.0005% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | G65E | 0.082% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | I172N | 0.006% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | L108R | 0.0003% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | P31L | 2.4% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | P31Q | 0.0001% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | R357W | 0.01% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | R409C | 0.4% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | T296N | 0.05% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | V282L | 2% of wild-type efficiency with substrate progesterone | Homo sapiens |
| 1.14.14.16 | W303R | 0.0001% of wild-type efficiency with substrate progesterone | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.16 | cytochrome b5 | - |
Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.16 | 0.00021 | - |
progesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |  |
| 1.14.14.16 | 0.00039 | - |
progesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.00044 | - |
17alpha-hydroxyprogesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.00046 | - |
17alpha-hydroxyprogesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0011 | - |
progesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0012 | - |
progesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0012 | - |
progesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0014 | - |
17alpha-hydroxyprogesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0015 | - |
17alpha-hydroxyprogesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0017 | - |
17alpha-hydroxyprogesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0017 | - |
17alpha-hydroxyprogesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0018 | - |
progesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0024 | - |
17alpha-hydroxyprogesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0027 | - |
progesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0036 | - |
17alpha-hydroxyprogesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0038 | - |
progesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0046 | - |
17alpha-hydroxyprogesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0047 | - |
progesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0049 | - |
17alpha-hydroxyprogesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0055 | - |
progesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0073 | - |
17alpha-hydroxyprogesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0096 | - |
progesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.013 | - |
17alpha-hydroxyprogesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.022 | - |
progesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.022 | - |
17alpha-hydroxyprogesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.11 | - |
progesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.16 | Homo sapiens | P08686 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.16 | 17alpha-hydroxyprogesterone + NADPH + H+ + O2 | - |
Homo sapiens | 17,21-dihydroxyprogesterone + NADP+ + H2O | - |
? | |
| 1.14.14.16 | additional information | C-H bond breaking is a rate-limiting step over a 10000fold range of catalytic efficiency | Homo sapiens | ? | - |
? | |
| 1.14.14.16 | progesterone + NADPH + H+ + O2 | - |
Homo sapiens | 11-deoxycorticosterone + NADP+ + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.16 | cytochrome p450 21A2 | - |
Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.16 | 0.00003 | - |
17alpha-hydroxyprogesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.000033 | - |
17alpha-hydroxyprogesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.00005 | - |
17alpha-hydroxyprogesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0001 | - |
progesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0001 | - |
progesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.00013 | - |
progesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.00022 | - |
17alpha-hydroxyprogesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.00027 | - |
progesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0005 | - |
17alpha-hydroxyprogesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.00083 | - |
progesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.0015 | - |
progesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.003 | - |
17alpha-hydroxyprogesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.008 | - |
progesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.02 | - |
progesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.028 | - |
17alpha-hydroxyprogesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.03 | - |
17alpha-hydroxyprogesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.032 | - |
17alpha-hydroxyprogesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.033 | - |
17alpha-hydroxyprogesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.06 | - |
progesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.063 | - |
17alpha-hydroxyprogesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.2 | - |
progesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.38 | - |
progesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 1.033 | - |
progesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 1.4 | - |
17alpha-hydroxyprogesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 2.8 | - |
progesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 4 | - |
17alpha-hydroxyprogesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.16 | 0.012 | - |
progesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.012 | - |
progesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.013 | - |
progesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.016 | - |
17alpha-hydroxyprogesterone | mutant W303R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.02 | - |
17alpha-hydroxyprogesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.02 | - |
17alpha-hydroxyprogesterone | mutant R357W, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.03 | - |
progesterone | mutant L108R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.03 | - |
17alpha-hydroxyprogesterone | mutant P31Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.07 | - |
progesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.12 | - |
17alpha-hydroxyprogesterone | mutant G292C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.62 | - |
17alpha-hydroxyprogesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 0.82 | - |
progesterone | mutant I172N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 2.3 | - |
17alpha-hydroxyprogesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 6.7 | - |
progesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 11.2 | - |
progesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 13.3 | - |
17alpha-hydroxyprogesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 43.3 | - |
progesterone | mutant G292S, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 50 | - |
progesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 61.7 | - |
17alpha-hydroxyprogesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 66.7 | - |
17alpha-hydroxyprogesterone | mutant R409C, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 100 | - |
17alpha-hydroxyprogesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 267 | - |
progesterone | mutant V282L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 317 | - |
progesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 817 | - |
17alpha-hydroxyprogesterone | mutant P31L, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 2667 | - |
17alpha-hydroxyprogesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.14.16 | 13333 | - |
progesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
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