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Literature summary extracted from

  • Moghal, A.; Hwang, L.; Faull, K.; Ibba, M.
    Multiple quality control pathways limit non-protein amino acid use by yeast cytoplasmic phenylalanyl-tRNA synthetase (2016), J. Biol. Chem., 291, 15796-15805 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.20 0.0423
-
 L-phenylalanine at pH 7.5 and 37°C Saccharomyces cerevisiae
6.1.1.20 0.3107
-
 2-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae
6.1.1.20 1.15
-
 3-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.20 cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Saccharomyces cerevisiae
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + 2-L-tyrosine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + 2-L-tyrosyl-tRNAPhe
-
 ?
6.1.1.20 ATP + 3-L-tyrosine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + 3-L-tyrosyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-alanine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + L-alanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-leucine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + L-leucyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 additional information very low activity with L-DOPA and 4-L-tyrosine Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.20 Phenylalanine-tRNA synthetase
-
 Saccharomyces cerevisiae
6.1.1.20 PheRS
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.20 26
-
 3-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae
6.1.1.20 39.4
-
 2-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae
6.1.1.20 58.2
-
 L-phenylalanine at pH 7.5 and 37°C Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.20 ATP
-
 Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.20 23
-
 3-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae
6.1.1.20 130
-
 2-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae
6.1.1.20 1400
-
 L-phenylalanine at pH 7.5 and 37°C Saccharomyces cerevisiae