BRENDA - Enzyme Database

Reduction of flavodoxin by electron bifurcation and sodium ion-dependent reoxidation by NAD+ catalyzed by ferredoxin-NAD+ reductase (Rnf)

Chowdhury, N.P.; Klomann, K.; Seubert, A.; Buckel, W.; J. Biol. Chem. 291, 11993-12002 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
7.2.1.2
expressed in Escherichia coli BL21(DE3) cells
Acidaminococcus fermentans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.1.3
0.0014
-
oxidized [2Fe-2S] ferredoxin
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
1.18.1.3
0.002
-
flavodoxin hydroquinone
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
7.2.1.2
0.00026
-
reduced ferredoxin [iron-sulfur] cluster
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
7.2.1.2
0.00042
-
reduced flavodoxin [iron-sulfur] cluster
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.18.1.3
membrane
-
Acidaminococcus fermentans
16020
-
7.2.1.2
membrane
-
Acidaminococcus fermentans
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.1.3
Li+
required for activity
Acidaminococcus fermentans
1.18.1.3
Na+
required for activity
Acidaminococcus fermentans
7.2.1.2
Na+
required for activity
Acidaminococcus fermentans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.1.3
flavodoxin hydroquinone + NADH
Acidaminococcus fermentans
-
flavodoxin hydroquinol + NAD+ + H+
-
-
ir
1.18.1.3
flavodoxin hydroquinone + NADH
Acidaminococcus fermentans DSM 20731
-
flavodoxin hydroquinol + NAD+ + H+
-
-
ir
1.18.1.3
flavodoxin hydroquinone + NADH
Acidaminococcus fermentans VR4
-
flavodoxin hydroquinol + NAD+ + H+
-
-
ir
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
Acidaminococcus fermentans
-
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
Acidaminococcus fermentans DSM 20731
-
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
Acidaminococcus fermentans VR4
-
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
?
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans DSM 20731
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans VR4
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.1.3
Acidaminococcus fermentans
-
-
-
1.18.1.3
Acidaminococcus fermentans DSM 20731
-
-
-
7.2.1.2
Acidaminococcus fermentans
-
-
-
7.2.1.2
Acidaminococcus fermentans VR4
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.1.3
flavodoxin hydroquinone + NADH
-
745340
Acidaminococcus fermentans
flavodoxin hydroquinol + NAD+ + H+
-
-
-
ir
1.18.1.3
flavodoxin hydroquinone + NADH
-
745340
Acidaminococcus fermentans DSM 20731
flavodoxin hydroquinol + NAD+ + H+
-
-
-
ir
1.18.1.3
flavodoxin hydroquinone + NADH
-
745340
Acidaminococcus fermentans VR4
flavodoxin hydroquinol + NAD+ + H+
-
-
-
ir
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
-
745340
Acidaminococcus fermentans
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
-
745340
Acidaminococcus fermentans DSM 20731
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
-
745340
Acidaminococcus fermentans VR4
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
-
?
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans DSM 20731
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans VR4
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
7.2.1.2
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
7.2.1.2
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans DSM 20731
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
7.2.1.2
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans VR4
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.18.1.3
FAD
-
Acidaminococcus fermentans
1.18.1.3
NAD+
-
Acidaminococcus fermentans
7.2.1.2
FMN
-
Acidaminococcus fermentans
7.2.1.2
NAD+
-
Acidaminococcus fermentans
7.2.1.2
[4Fe-4S]-center
-
Acidaminococcus fermentans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.2.1.2
expressed in Escherichia coli BL21(DE3) cells
Acidaminococcus fermentans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.18.1.3
FAD
-
Acidaminococcus fermentans
1.18.1.3
NAD+
-
Acidaminococcus fermentans
7.2.1.2
FMN
-
Acidaminococcus fermentans
7.2.1.2
NAD+
-
Acidaminococcus fermentans
7.2.1.2
[4Fe-4S]-center
-
Acidaminococcus fermentans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.1.3
0.0014
-
oxidized [2Fe-2S] ferredoxin
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
1.18.1.3
0.002
-
flavodoxin hydroquinone
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
7.2.1.2
0.00026
-
reduced ferredoxin [iron-sulfur] cluster
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
7.2.1.2
0.00042
-
reduced flavodoxin [iron-sulfur] cluster
at pH 6.8, temperature not specified in the publication
Acidaminococcus fermentans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.18.1.3
membrane
-
Acidaminococcus fermentans
16020
-
7.2.1.2
membrane
-
Acidaminococcus fermentans
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.1.3
Li+
required for activity
Acidaminococcus fermentans
1.18.1.3
Na+
required for activity
Acidaminococcus fermentans
7.2.1.2
Na+
required for activity
Acidaminococcus fermentans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.1.3
flavodoxin hydroquinone + NADH
Acidaminococcus fermentans
-
flavodoxin hydroquinol + NAD+ + H+
-
-
ir
1.18.1.3
flavodoxin hydroquinone + NADH
Acidaminococcus fermentans DSM 20731
-
flavodoxin hydroquinol + NAD+ + H+
-
-
ir
1.18.1.3
flavodoxin hydroquinone + NADH
Acidaminococcus fermentans VR4
-
flavodoxin hydroquinol + NAD+ + H+
-
-
ir
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
Acidaminococcus fermentans
-
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
Acidaminococcus fermentans DSM 20731
-
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
Acidaminococcus fermentans VR4
-
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
?
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans DSM 20731
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
Acidaminococcus fermentans VR4
-
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ir
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.1.3
flavodoxin hydroquinone + NADH
-
745340
Acidaminococcus fermentans
flavodoxin hydroquinol + NAD+ + H+
-
-
-
ir
1.18.1.3
flavodoxin hydroquinone + NADH
-
745340
Acidaminococcus fermentans DSM 20731
flavodoxin hydroquinol + NAD+ + H+
-
-
-
ir
1.18.1.3
flavodoxin hydroquinone + NADH
-
745340
Acidaminococcus fermentans VR4
flavodoxin hydroquinol + NAD+ + H+
-
-
-
ir
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
-
745340
Acidaminococcus fermentans
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
-
745340
Acidaminococcus fermentans DSM 20731
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
-
745340
Acidaminococcus fermentans VR4
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
-
?
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans DSM 20731
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
7.2.1.2
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans VR4
oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
7.2.1.2
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
7.2.1.2
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans DSM 20731
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir
7.2.1.2
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
745340
Acidaminococcus fermentans VR4
oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
-
ir