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Literature summary extracted from

  • Chowdhury, N.P.; Klomann, K.; Seubert, A.; Buckel, W.
    Reduction of flavodoxin by electron bifurcation and sodium ion-dependent reoxidation by NAD+ catalyzed by ferredoxin-NAD+ reductase (Rnf) (2016), J. Biol. Chem., 291, 11993-12002 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.1.2 expressed in Escherichia coli BL21(DE3) cells Acidaminococcus fermentans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.3 0.0014
-
 oxidized [2Fe-2S] ferredoxin at pH 6.8, temperature not specified in the publication Acidaminococcus fermentans
1.18.1.3 0.002
-
 flavodoxin hydroquinone at pH 6.8, temperature not specified in the publication Acidaminococcus fermentans
7.2.1.2 0.00026
-
 reduced ferredoxin [iron-sulfur] cluster at pH 6.8, temperature not specified in the publication Acidaminococcus fermentans
7.2.1.2 0.00042
-
 reduced flavodoxin [iron-sulfur] cluster at pH 6.8, temperature not specified in the publication Acidaminococcus fermentans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.3 membrane
-
 Acidaminococcus fermentans 16020
-
7.2.1.2 membrane
-
 Acidaminococcus fermentans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.1.3 Li+ required for activity Acidaminococcus fermentans
1.18.1.3 Na+ required for activity Acidaminococcus fermentans
7.2.1.2 Na+ required for activity Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.3 flavodoxin hydroquinone + NADH Acidaminococcus fermentans
-
 flavodoxin hydroquinol + NAD+ + H+
-
 ir
1.18.1.3 flavodoxin hydroquinone + NADH Acidaminococcus fermentans VR4
-
 flavodoxin hydroquinol + NAD+ + H+
-
 ir
1.18.1.3 flavodoxin hydroquinone + NADH Acidaminococcus fermentans DSM 20731
-
 flavodoxin hydroquinol + NAD+ + H+
-
 ir
1.18.1.3 oxidized [2Fe-2S] ferredoxin + NADH Acidaminococcus fermentans
-
 reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
 ?
1.18.1.3 oxidized [2Fe-2S] ferredoxin + NADH Acidaminococcus fermentans VR4
-
 reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
 ?
1.18.1.3 oxidized [2Fe-2S] ferredoxin + NADH Acidaminococcus fermentans DSM 20731
-
 reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
 ?
7.2.1.2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1] Acidaminococcus fermentans
-
 oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
 ir
7.2.1.2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1] Acidaminococcus fermentans VR4
-
 oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
 ir
7.2.1.2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1] Acidaminococcus fermentans DSM 20731
-
 oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.3 Acidaminococcus fermentans
-
-
-
1.18.1.3 Acidaminococcus fermentans DSM 20731
-
-
-
7.2.1.2 Acidaminococcus fermentans
-
-
-
7.2.1.2 Acidaminococcus fermentans VR4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.3 flavodoxin hydroquinone + NADH
-
 Acidaminococcus fermentans flavodoxin hydroquinol + NAD+ + H+
-
 ir
1.18.1.3 flavodoxin hydroquinone + NADH
-
 Acidaminococcus fermentans VR4 flavodoxin hydroquinol + NAD+ + H+
-
 ir
1.18.1.3 flavodoxin hydroquinone + NADH
-
 Acidaminococcus fermentans DSM 20731 flavodoxin hydroquinol + NAD+ + H+
-
 ir
1.18.1.3 oxidized [2Fe-2S] ferredoxin + NADH
-
 Acidaminococcus fermentans reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
 ?
1.18.1.3 oxidized [2Fe-2S] ferredoxin + NADH
-
 Acidaminococcus fermentans VR4 reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
 ?
1.18.1.3 oxidized [2Fe-2S] ferredoxin + NADH
-
 Acidaminococcus fermentans DSM 20731 reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
 ?
7.2.1.2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
 Acidaminococcus fermentans oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
 ir
7.2.1.2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
 Acidaminococcus fermentans VR4 oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
 ir
7.2.1.2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
 Acidaminococcus fermentans DSM 20731 oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
 ir
7.2.1.2 reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
 Acidaminococcus fermentans oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
 ir
7.2.1.2 reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
 Acidaminococcus fermentans VR4 oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
 ir
7.2.1.2 reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]
-
 Acidaminococcus fermentans DSM 20731 oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.18.1.3 ferredoxin-NAD+ reductase
-
 Acidaminococcus fermentans
1.18.1.3 ferredoxin/flavodoxin-NAD+ reductase
-
 Acidaminococcus fermentans
1.18.1.3 Rnf
-
 Acidaminococcus fermentans
7.2.1.2 Rnf complex
-
 Acidaminococcus fermentans
7.2.1.2 rnfABCDEG
-
 Acidaminococcus fermentans

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.3 FAD
-
 Acidaminococcus fermentans
1.18.1.3 NAD+
-
 Acidaminococcus fermentans
7.2.1.2 FMN
-
 Acidaminococcus fermentans
7.2.1.2 NAD+
-
 Acidaminococcus fermentans
7.2.1.2 [4Fe-4S]-center
-
 Acidaminococcus fermentans