BRENDA - Enzyme Database

Plasmodium apicoplast Gln-tRNAGln biosynthesis utilizes a unique GatAB amidotransferase essential for erythrocytic stage parasites

Mailu, B.M.; Li, L.; Arthur, J.; Nelson, T.M.; Ramasamy, G.; Fritz-Wolf, K.; Becker, K.; Gardner, M.J.; J. Biol. Chem. 290, 29629-29641 (2015)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
6.1.1.24
drug development
the apicoplast indirect aminoacylation pathway is a potential drug target
Plasmodium falciparum
Cloned(Commentary)
EC Number
Commentary
Organism
6.1.1.24
gene Pf3D7_1357200, recombinant expression of codon-optimized GluRS in Escherichia coli KRX cells
Plasmodium falciparum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.24
apicoplast
-
Plasmodium falciparum
20011
-
6.3.5.7
apicoplast
in blood stage parasites, heterodimeric glutamyl-tRNA amidotransferase consisting of GatA and GatB subunits
Plasmodium berghei
20011
-
6.3.5.7
apicoplast
in blood stage parasites, heterodimeric glutamyl-tRNA amidotransferase consisting of GatA and GatB subunits
Plasmodium falciparum
20011
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.1.1.24
Mg2+
required
Plasmodium falciparum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGln
Plasmodium falciparum
-
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
?
6.1.1.24
ATP + L-glutamate + tRNAGlu
Plasmodium falciparum
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
-
?
6.1.1.24
additional information
Plasmodium falciparum
the glutamyl residue of Glu-tRNAGln is then transamidated by a glutamyl-tRNAGln amidotransferase (Glu-AdT) in the presence of ATP using Gln as an amide donor, producing GlntRNAGln, coupled reaction assay
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.24
Plasmodium falciparum
Q8IDD3
-
-
6.3.5.7
Plasmodium berghei
A0A077X9J8 and A0A077XDD6
A0A077X9J8 i.e. subunit GatA, A0A077XDD6 i.e. subunit GatB
-
6.3.5.7
Plasmodium berghei ANKA
A0A077X9J8 and A0A077XDD6
A0A077X9J8 i.e. subunit GatA, A0A077XDD6 i.e. subunit GatB
-
6.3.5.7
Plasmodium falciparum
Q8I1S6 and C6KTC3
Q8I1S6 i.e. subunit GatA, C6KTC3 i.e. subunit GatB
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGln
-
745335
Plasmodium falciparum
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
-
?
6.1.1.24
ATP + L-glutamate + tRNAGlu
-
745335
Plasmodium falciparum
AMP + diphosphate + L-glutamyl-tRNAGlu
-
-
-
?
6.1.1.24
additional information
the glutamyl residue of Glu-tRNAGln is then transamidated by a glutamyl-tRNAGln amidotransferase (Glu-AdT) in the presence of ATP using Gln as an amide donor, producing GlntRNAGln, coupled reaction assay
745335
Plasmodium falciparum
?
-
-
-
-
6.1.1.24
additional information
the non-discriminating GluRS (ND-GluRS) can glutamylate both tRNAGlu and tRNAGln
745335
Plasmodium falciparum
?
-
-
-
-
6.3.5.7
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium falciparum
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium berghei
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium berghei ANKA
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
additional information
no substrate: L-glutamate
745335
Plasmodium falciparum
?
-
-
-
-
6.3.5.7
additional information
no substrate: L-glutamate
745335
Plasmodium berghei
?
-
-
-
-
6.3.5.7
additional information
no substrate: L-glutamate
745335
Plasmodium berghei ANKA
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
6.3.5.7
dimer
1 * 85000, subunit GatA, 1 * 94500, subunit GatB, SDS-PAGE
Plasmodium falciparum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.1.1.24
37
-
assay at
Plasmodium falciparum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.24
ATP
-
Plasmodium falciparum
Application (protein specific)
EC Number
Application
Commentary
Organism
6.1.1.24
drug development
the apicoplast indirect aminoacylation pathway is a potential drug target
Plasmodium falciparum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.24
gene Pf3D7_1357200, recombinant expression of codon-optimized GluRS in Escherichia coli KRX cells
Plasmodium falciparum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.24
ATP
-
Plasmodium falciparum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.24
apicoplast
-
Plasmodium falciparum
20011
-
6.3.5.7
apicoplast
in blood stage parasites, heterodimeric glutamyl-tRNA amidotransferase consisting of GatA and GatB subunits
Plasmodium berghei
20011
-
6.3.5.7
apicoplast
in blood stage parasites, heterodimeric glutamyl-tRNA amidotransferase consisting of GatA and GatB subunits
Plasmodium falciparum
20011
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.1.1.24
Mg2+
required
Plasmodium falciparum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGln
Plasmodium falciparum
-
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
?
6.1.1.24
ATP + L-glutamate + tRNAGlu
Plasmodium falciparum
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
-
?
6.1.1.24
additional information
Plasmodium falciparum
the glutamyl residue of Glu-tRNAGln is then transamidated by a glutamyl-tRNAGln amidotransferase (Glu-AdT) in the presence of ATP using Gln as an amide donor, producing GlntRNAGln, coupled reaction assay
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGln
-
745335
Plasmodium falciparum
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
-
?
6.1.1.24
ATP + L-glutamate + tRNAGlu
-
745335
Plasmodium falciparum
AMP + diphosphate + L-glutamyl-tRNAGlu
-
-
-
?
6.1.1.24
additional information
the glutamyl residue of Glu-tRNAGln is then transamidated by a glutamyl-tRNAGln amidotransferase (Glu-AdT) in the presence of ATP using Gln as an amide donor, producing GlntRNAGln, coupled reaction assay
745335
Plasmodium falciparum
?
-
-
-
-
6.1.1.24
additional information
the non-discriminating GluRS (ND-GluRS) can glutamylate both tRNAGlu and tRNAGln
745335
Plasmodium falciparum
?
-
-
-
-
6.3.5.7
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium falciparum
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium berghei
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium berghei ANKA
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
additional information
no substrate: L-glutamate
745335
Plasmodium falciparum
?
-
-
-
-
6.3.5.7
additional information
no substrate: L-glutamate
745335
Plasmodium berghei
?
-
-
-
-
6.3.5.7
additional information
no substrate: L-glutamate
745335
Plasmodium berghei ANKA
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.5.7
dimer
1 * 85000, subunit GatA, 1 * 94500, subunit GatB, SDS-PAGE
Plasmodium falciparum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.1.1.24
37
-
assay at
Plasmodium falciparum
General Information
EC Number
General Information
Commentary
Organism
6.1.1.24
metabolism
the malaria parasite Plasmodium falciparum apicoplast indirect aminoacylation pathway utilizes a non-discriminating glutamyl-tRNA synthetase to synthesize Glu-tRNAGln and a glutaminyl-tRNA amidotransferase to convert Glu-tRNAGln to Gln-tRNAGln
Plasmodium falciparum
6.1.1.24
physiological function
Plasmodium apicoplast protein synthesis is essential for parasite survival, aminoacyl-tRNA formation is essential for protein synthesis. The malaria parasite Plasmodium falciparum apicoplast indirect aminoacylation pathway utilizes a non-discriminating glutamyl-tRNA synthetase to synthesize Glu-tRNAGln and a glutaminyl-tRNA amidotransferase to convert Glu-tRNAGln to Gln-tRNAGln. Formation of apicoplast Gln-tRNAGln proceeds via indirect aminoacylation. The nucleus-encoded non-discriminating GluRS is imported into the apicoplast is responsible for the formation of misacylated Glu-tRNAGln and is essential in the erythrocytic stages
Plasmodium falciparum
6.3.5.7
physiological function
gene encoding GatA is essential to the Plasmodium berghei blood stages
Plasmodium berghei
General Information (protein specific)
EC Number
General Information
Commentary
Organism
6.1.1.24
metabolism
the malaria parasite Plasmodium falciparum apicoplast indirect aminoacylation pathway utilizes a non-discriminating glutamyl-tRNA synthetase to synthesize Glu-tRNAGln and a glutaminyl-tRNA amidotransferase to convert Glu-tRNAGln to Gln-tRNAGln
Plasmodium falciparum
6.1.1.24
physiological function
Plasmodium apicoplast protein synthesis is essential for parasite survival, aminoacyl-tRNA formation is essential for protein synthesis. The malaria parasite Plasmodium falciparum apicoplast indirect aminoacylation pathway utilizes a non-discriminating glutamyl-tRNA synthetase to synthesize Glu-tRNAGln and a glutaminyl-tRNA amidotransferase to convert Glu-tRNAGln to Gln-tRNAGln. Formation of apicoplast Gln-tRNAGln proceeds via indirect aminoacylation. The nucleus-encoded non-discriminating GluRS is imported into the apicoplast is responsible for the formation of misacylated Glu-tRNAGln and is essential in the erythrocytic stages
Plasmodium falciparum
6.3.5.7
physiological function
gene encoding GatA is essential to the Plasmodium berghei blood stages
Plasmodium berghei