EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.12.16 | gene pa4202, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta(DE3)pLysS | Pseudomonas aeruginosa |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.12.16 | purified recombinant His-tagged enzyme, vapor diffusion hanging drop method at room temperature, 0.002 ml of 14 mg/ml protein in 50 mM Tris-Cl, pH 8.0, 100 mM NaCl, 20 mM 2-nitropropane, with 0.002 ml of reservoir solution containing 14% w/v PEG 5000 monomethylether and 0.1 M Na-HEPES, pH 7.0, and equilibration against 1 ml of reservoir solution, 7-10 days, X-ray diffraction structure determination and analysis at 1.44 A resolution, molecular replacement | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.12.16 | additional information | - |
additional information | steady-state kinetic mechanism | Pseudomonas aeruginosa | |
1.13.12.16 | 0.11 | - |
propionate-3-nitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.13.12.16 | 5 | - |
ethylnitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.13.12.16 | 6 | - |
propyl-1-nitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.16 | 3 propionate-3-nitronate + O2 | Pseudomonas aeruginosa | - |
3 malonic semialdehyde + nitrite + 2 nitrate + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.12.16 | Pseudomonas aeruginosa | Q9HWH9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.12.16 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta(DE3)pLysS by affinity chromatography | Pseudomonas aeruginosa |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.13.12.16 | ethylnitronate + O2 = acetaldehyde + nitrite + other products | catalyze the oxidation of propionate-3-nitronate or other nitronate analogues through a radical mechanism involving a catalytic flavosemiquinone without formation of a canonical C4a-(hydro)peroxyflavin | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.16 | 3 propionate-3-nitronate + O2 | - |
Pseudomonas aeruginosa | 3 malonic semialdehyde + nitrite + 2 nitrate + H2O2 | - |
? | |
1.13.12.16 | 3 propionate-3-nitronate + O2 | best substrate | Pseudomonas aeruginosa | 3 malonic semialdehyde + nitrite + 2 nitrate + H2O2 | - |
? | |
1.13.12.16 | butyl-1-nitronate + O2 | - |
Pseudomonas aeruginosa | ? | - |
? | |
1.13.12.16 | ethylnitronate + O2 | - |
Pseudomonas aeruginosa | acetaldehyde + nitrite + other products | - |
? | |
1.13.12.16 | additional information | the enzyme is active on propionate-3-nitronate and other alkyl nitronates, but cannot oxidize nitroalkanes, e.g. 3-nitropropionate, nitroethane, 1-nitropropane, 1-nitrobutane, 1-nitropentane, or 2-nitropropane. Anaerobic reduction of the enzyme with propionate-3-nitronate yields a flavosemiquinone | Pseudomonas aeruginosa | ? | - |
? | |
1.13.12.16 | pentyl-1-nitronate + O2 | - |
Pseudomonas aeruginosa | ? | - |
? | |
1.13.12.16 | propyl-1-nitronate + O2 | - |
Pseudomonas aeruginosa | ? | - |
? | |
1.13.12.16 | propyl-2-nitronate + O2 | - |
Pseudomonas aeruginosa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.12.16 | More | the dimer interface includes eight contacts mainly in the FMN-binding domain and it does not seem to be directly relevant for catalysis as it is far from the active site pocket, structure analysis, overview | Pseudomonas aeruginosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.12.16 | NMO | - |
Pseudomonas aeruginosa |
1.13.12.16 | Pa-NMO | - |
Pseudomonas aeruginosa |
1.13.12.16 | PA4202 | - |
Pseudomonas aeruginosa |
1.13.12.16 | protein PA4202 | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.12.16 | 30 | - |
assay at | Pseudomonas aeruginosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.12.16 | 350 | - |
ethylnitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.13.12.16 | 1120 | - |
propyl-1-nitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.13.12.16 | 1300 | - |
propionate-3-nitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.12.16 | 7.5 | - |
assay at | Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.13.12.16 | FMN | dependent on, FMN-binding domain structure, overview | Pseudomonas aeruginosa |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.12.16 | evolution | NMO is a member of the group H flavin-dependent monooxygenases | Pseudomonas aeruginosa |
1.13.12.16 | additional information | four conserved motifs are identified in PA4202. Oxidation of propionate-3-nitronate by NMO yields malonic semialdehyde, which is an important metabolite that can be converted to acetyl-CoA, acetate, or 3-hydroxypropionate and enter various catabolic or anabolic pathways. Active site structure analysis, overview | Pseudomonas aeruginosa |
1.13.12.16 | physiological function | nitronate monooxygenase oxidizes the mitochondrial toxin propionate 3-nitronate to malonate semialdehyde, and detoxifies the deadly toxin | Pseudomonas aeruginosa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.12.16 | 29 | - |
propyl-2-nitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.13.12.16 | 33 | - |
butyl-1-nitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.13.12.16 | 70 | - |
ethylnitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.13.12.16 | 91 | - |
pentyl-1-nitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.13.12.16 | 186.71 | - |
propyl-1-nitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.13.12.16 | 11818.2 | - |
propionate-3-nitronate | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa |