Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Greenfield, L.; Richards, M.; Li, J.; Wakarchuk, W.; Lowary, T.; Whitfield, C.
    Biosynthesis of the polymannose lipopolysaccharide O-antigens from Escherichia coli serotypes O8 and O9a requires a unique combination of single- and multiple-active site mannosyltransferases (2012), J. Biol. Chem., 287, 35078-35091 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.348 expressed in Escherichia coli Top10 cells Escherichia coli
2.4.1.349 expressed in Escherichia coli Top10 cells Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.371 95500
-
 gel filtration Escherichia coli
2.4.1.371 137000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.348 GDP-alpha-D-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli
-
 GDP + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.348 GDP-alpha-D-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli CWG636
-
 GDP + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.348 GDP-alpha-D-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli CWG634
-
 GDP + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.349 GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli
-
 GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.349 GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli CWG636
-
 GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.349 GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli CWG634
-
 GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.348 Escherichia coli
-
-
-
2.4.1.348 Escherichia coli CWG634
-
-
-
2.4.1.348 Escherichia coli CWG636
-
-
-
2.4.1.349 Escherichia coli
-
-
-
2.4.1.349 Escherichia coli CWG634
-
-
-
2.4.1.349 Escherichia coli CWG636
-
-
-
2.4.1.371 Escherichia coli
-
 serotype O9a
-
2.4.1.371 Escherichia coli
-
 serotype O8
-
2.4.1.371 Escherichia coli CWG634
-
 serotype O9a
-
2.4.1.371 Escherichia coli CWG636
-
 serotype O8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.348 amylose resin column chromatography Escherichia coli
2.4.1.349 amylose resin column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.348 GDP-alpha-D-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Escherichia coli GDP + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.348 GDP-alpha-D-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Escherichia coli CWG636 GDP + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.348 GDP-alpha-D-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Escherichia coli CWG634 GDP + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.349 GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Escherichia coli GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.349 GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Escherichia coli CWG636 GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.349 GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Escherichia coli CWG634 GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.371 2 GDP-alpha-D-mannose + alpha-Manp-(1->2)-alpha-Manp-(1->2)-alpha-Manp-(1->3)-alpha-Manp the enzyme polymerizes a tetrasaccharide repeat unit containing two alpha-(1->3)- and two alpha-(1->2)-linked mannopyranose residues Escherichia coli 2 GDP + ?
-
 ?
2.4.1.371 2 GDP-alpha-D-mannose + alpha-Manp-(1->2)-alpha-Manp-(1->2)-alpha-Manp-(1->3)-alpha-Manp the enzyme polymerizes a tetrasaccharide repeat unit containing two alpha-(1->3)- and two alpha-(1->2)-linked mannopyranose residues Escherichia coli CWG636 2 GDP + ?
-
 ?
2.4.1.371 2 GDP-alpha-D-mannose + alpha-Manp-(1->2)-alpha-Manp-(1->2)-alpha-Manp-(1->3)-alpha-Manp the enzyme polymerizes a tetrasaccharide repeat unit containing two alpha-(1->3)- and two alpha-(1->2)-linked mannopyranose residues Escherichia coli CWG634 2 GDP + ?
-
 ?
2.4.1.371 2 GDP-alpha-D-mannose + alpha-Manp-(1->3)-alpha-Manp-(1->3)-beta-GlcpNAc the enzyme polymerizes trisaccharide repeat units containing single alpha-(1->3)-, alpha-(1->2)-, and beta-(1->2)-mannopyranoses Escherichia coli 2 GDP + ?
-
 ?
2.4.1.371 2 GDP-alpha-D-mannose + alpha-Manp-(1->3)-alpha-Manp-(1->3)-beta-GlcpNAc the enzyme polymerizes trisaccharide repeat units containing single alpha-(1->3)-, alpha-(1->2)-, and beta-(1->2)-mannopyranoses Escherichia coli CWG636 2 GDP + ?
-
 ?
2.4.1.371 2 GDP-alpha-D-mannose + alpha-Manp-(1->3)-alpha-Manp-(1->3)-beta-GlcpNAc the enzyme polymerizes trisaccharide repeat units containing single alpha-(1->3)-, alpha-(1->2)-, and beta-(1->2)-mannopyranoses Escherichia coli CWG634 2 GDP + ?
-
 ?
2.4.1.371 additional information no activity with alpha-Manp-(1->3)-alpha-Manp-(1->3)-beta-GlcpNAc Escherichia coli ?
-
-
2.4.1.371 additional information no activity with alpha-Manp-(1->3)-alpha-Manp-(1->3)-beta-GlcpNAc Escherichia coli CWG636 ?
-
-
2.4.1.371 additional information no activity with alpha-Manp-(1->3)-alpha-Manp-(1->3)-beta-GlcpNAc Escherichia coli CWG634 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.348 WbdC
-
 Escherichia coli
2.4.1.349 WbdB
-
 Escherichia coli
2.4.1.371 WbdA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.371 25
-
-
 Escherichia coli