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Literature summary extracted from

  • Frias, J.; Richman, J.; Erickson, J.; Wackett, L.
    Purification and characterization of OleA from Xanthomonas campestris and demonstration of a non-decarboxylative claisen condensation reaction (2011), J. Biol. Chem., 286, 10930-10938 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.20 expressed in Escherichia coli One Shot BL21(DE3) cells Xanthomonas campestris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.20 62000
-
 gel filtration Xanthomonas campestris

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.20 Xanthomonas campestris
-
-
-
2.3.3.20 Xanthomonas campestris ATCC 33913
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.20 HisTrap column chromatography Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.20 decanoyl-CoA + H2O 42% activity compared to palmitoyl-CoA Xanthomonas campestris ?
-
 ?
2.3.3.20 decanoyl-CoA + H2O 42% activity compared to palmitoyl-CoA Xanthomonas campestris ATCC 33913 ?
-
 ?
2.3.3.20 lauroyl-CoA + H2O 79% activity compared to palmitoyl-CoA Xanthomonas campestris ?
-
 ?
2.3.3.20 lauroyl-CoA + H2O 79% activity compared to palmitoyl-CoA Xanthomonas campestris ATCC 33913 ?
-
 ?
2.3.3.20 additional information no activity with acetyl-CoA Xanthomonas campestris ?
-
 ?
2.3.3.20 additional information no activity with acetyl-CoA Xanthomonas campestris ATCC 33913 ?
-
 ?
2.3.3.20 myristoyl-CoA + H2O 97% activity compared to palmitoyl-CoA Xanthomonas campestris 2-myristoylmyristic acid + myristic acid + 14-heptacosanone + CoA
-
 ?
2.3.3.20 myristoyl-CoA + H2O 97% activity compared to palmitoyl-CoA Xanthomonas campestris ATCC 33913 2-myristoylmyristic acid + myristic acid + 14-heptacosanone + CoA
-
 ?
2.3.3.20 octanoyl-CoA + H2O 12% activity compared to palmitoyl-CoA Xanthomonas campestris ?
-
 ?
2.3.3.20 octanoyl-CoA + H2O 12% activity compared to palmitoyl-CoA Xanthomonas campestris ATCC 33913 ?
-
 ?
2.3.3.20 palmitoleoyl-CoA + H2O 57% activity compared to palmitoyl-CoA Xanthomonas campestris ?
-
 ?
2.3.3.20 palmitoyl-CoA + H2O 100% activity Xanthomonas campestris ?
-
 ?
2.3.3.20 stearoyl-CoA + H2O 29% activity compared to palmitoyl-CoA Xanthomonas campestris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.20 homodimer 2 * 36629, calculated from amino acid sequence Xanthomonas campestris
2.3.3.20 homodimer 2 * 40000, His-tagged enzyme, SDS-PAGE Xanthomonas campestris

Synonyms

EC Number Synonyms Comment Organism
2.3.3.20 oleA
-
 Xanthomonas campestris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.3.20 Xanthomonas campestris calculated from amino acid sequence
-
 5.6