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Literature summary extracted from

  • Massant, J.; Verstreken, P.; Durbec, V.; Kholti, A.; Legrain, C.; Beeckmans, S.; Cornelis, P.; Glansdorff, N.
    Metabolic channeling of carbamoyl phosphate, a thermolabile intermediate Evidence for physical interaction between carbamate kinase-like carbamoyl-phosphate synthetase and ornithine carbamoyltransferase from the hyperthermophile Pyrococcus furiosus (2002), J. Biol. Chem., 277, 18517-18522 .
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Pyrococcus furiosus Q51742
-
-
2.7.2.2 Pyrococcus furiosus P95474
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3
-
 Pyrococcus furiosus
2.7.2.2
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Pyrococcus furiosus phosphate + L-citrulline
-
 ?
2.7.2.2 ATP + NH3 + CO2
-
 Pyrococcus furiosus ADP + carbamoyl phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.3.3 otcase
-
 Pyrococcus furiosus
2.7.2.2 CKase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.3.3 60
-
 assay at Pyrococcus furiosus
2.7.2.2 60
-
 assay at Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 7.2
-
 assay at Pyrococcus furiosus
2.7.2.2 7.2
-
 assay at Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
2.1.3.3 metabolism carbamoyl-phosphate synthetase and ornithine carbamoyltransferase form an efficient channeling cluster for carbamoyl phosphate, an extremely thermolabile and potentially toxic metabolic intermediate. Therefore, by physically interacting with each other, carbamoyl-phosphate synthetase and ornithine carbamoyltransferase prevent the thermodenaturation of carbamoyl phosphate in the aqueous cytoplasmic environment Pyrococcus furiosus
2.7.2.2 metabolism carbamoyl-phosphate synthetase and ornithine carbamoyltransferase form an efficient channeling cluster for carbamoyl phosphate, an extremely thermolabile and potentially toxic metabolic intermediate. Therefore, by physically interacting with each other, carbamoyl-phosphate synthetase and ornithine carbamoyltransferase prevent the thermodenaturation of carbamoyl phosphate in the aqueous cytoplasmic environment Pyrococcus furiosus