EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.19.1 | analysis | the enzyme is expected to be useful for the removal of Nalpha-acylated residues in short peptide sequence analysis at high temperatures | Pyrococcus horikoshii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.19.1 | overexpressed in Escherichia coli BL21(DE3) | Pyrococcus horikoshii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.19.1 | 7.6 | - |
Acetyl-Ala-Ala | pH 5.4, 85°C | Pyrococcus horikoshii | |
3.4.19.1 | 8.6 | - |
Acetyl-Ala-Ala | pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii | |
3.4.19.1 | 11 | - |
acetyl-Leu-4-nitroanilide | pH 5.4, 85°C | Pyrococcus horikoshii | |
3.4.19.1 | 12.9 | - |
acetyl-Leu-4-nitroanilide | pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii | |
3.4.19.1 | 13 | - |
Acetyl-Ala-Ala-Ala-Ala | pH 5.4, 85°C | Pyrococcus horikoshii | |
3.4.19.1 | 15.2 | - |
Acetyl-Ala-Ala-Ala-Ala | pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii | |
3.4.19.1 | 18.4 | - |
acetyl-Ala-4-nitroanilide | pH 5.4, 85°C | Pyrococcus horikoshii | |
3.4.19.1 | 20.2 | - |
acetyl-Ala-4-nitroanilide | pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.19.1 | 70000 | - |
gel filtration | Pyrococcus horikoshii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.19.1 | Pyrococcus horikoshii | AB009494 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.19.1 | - |
Pyrococcus horikoshii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.19.1 | additional information | - |
the specific activity of the enzyme increases 714fold with heat treatment, suggesting the modification of the structure of the enzyme for optimal hydrolytic activity by heating | Pyrococcus horikoshii |
3.4.19.1 | 1.9 | - |
substrate: acetyl-Tyr-4-nitroanilide, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 6.9 | - |
substrate: acetyl-Ala-4-nitroanilide, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 9.5 | - |
substrate: formyl-Met-Ala-Ala-Ala-Ala-Ala, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 10.2 | - |
substrate: formyl-Ala-Ala-Ala-Ala-Ala-Ala, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 11.7 | - |
substrate: acetyl-Met-Ala-Ala-Ala-Ala-Ala, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 13.6 | - |
substrate: acetyl-Ala-Ala-Ala-Ala-Ala-Ala, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 15 | - |
substrate: acetyl-Tyr-4-nitroanilide, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 51 | - |
substrate: acetyl-Ala-4-nitroanilide, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 66.8 | - |
substrate: formyl-Met-Ala-Ala-Ala-Ala-Ala, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 77 | - |
substrate: acetyl-Met-Ala-Ala-Ala-Ala-Ala, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 77.3 | - |
substrate: formyl-Ala-Ala-Ala-Ala-Ala-Ala, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 97.2 | - |
substrate: formyl-Met-Ala, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 105 | - |
substrate: acetyl-Leu-4-nitroanilide, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 118 | - |
substrate: acetyl-Ala-Ala-Ala-Ala-Ala-Ala, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 206 | - |
substrate: acetyl-Ala-Ala-Ala-Ala, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 344 | - |
substrate: acetyl-Met-Ala, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 402 | - |
substrate: formyl-Ala-Ala-Ala, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 478 | - |
substrate: acetyl-Ala-Ala, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 694 | - |
substrate: formyl-Met-Leu-Gly, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 750 | - |
substrate: acetyl-Leu-4-nitroanilide, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 1388 | - |
substrate: formyl-Met-Ala-Ser, pH 5.4, 85°C | Pyrococcus horikoshii |
3.4.19.1 | 1513 | - |
substrate: formyl-Met-Ala, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 1830 | - |
substrate: acetyl-Ala-Ala-Ala-Ala, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 5120 | - |
substrate: acetyl-Met-Ala, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 5363 | - |
substrate: formyl-Ala-Ala-Ala, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 7060 | - |
substrate: acetyl-Ala-Ala, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 7064 | - |
substrate: formyl-Met-Leu-Gly, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
3.4.19.1 | 17026 | - |
substrate: formyl-Met-Ala-Ser, pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.19.1 | acetyl-Ala-4-nitroanilide + H2O | the enzyme releases acetyl-Leu better than acetyl-Ala from acetyl-amino acid-4-nitroanilides | Pyrococcus horikoshii | acetyl-Ala + 4-nitroaniline | - |
? | |
3.4.19.1 | acetyl-Ala-Ala + H2O | - |
Pyrococcus horikoshii | acetyl-Ala + Ala | - |
? | |
3.4.19.1 | acetyl-Ala-Ala-Ala-Ala + H2O | - |
Pyrococcus horikoshii | acetyl-Ala + Ala-Ala-Ala | - |
? | |
3.4.19.1 | acetyl-Ala-Ala-Ala-Ala-Ala-Ala + H2O | - |
Pyrococcus horikoshii | acetyl-Ala + Ala-Ala-Ala-Ala-Ala | - |
? | |
3.4.19.1 | acetyl-Leu-4-nitroanilide + H2O | the enzyme releases acetyl-Leu better than acetyl-Ala from acetyl-amino acid-4-nitroanilides | Pyrococcus horikoshii | acetyl-Leu + 4-nitroaniline | - |
? | |
3.4.19.1 | acetyl-Met-Ala + H2O | - |
Pyrococcus horikoshii | acetyl-Met + Ala | - |
? | |
3.4.19.1 | acetyl-Met-Ala-Ala-Ala-Ala-Ala + H2O | - |
Pyrococcus horikoshii | acetyl-Met + Ala-Ala-Ala-Ala-Ala | - |
? | |
3.4.19.1 | acetyl-Tyr-4-nitroanilide + H2O | - |
Pyrococcus horikoshii | acetyl-Tyr + 4-nitroaniline | - |
? | |
3.4.19.1 | formyl-Ala-Ala-Ala + H2O | - |
Pyrococcus horikoshii | formyl-Ala + Ala-Ala | - |
? | |
3.4.19.1 | formyl-Ala-Ala-Ala-Ala-Ala-Ala + H2O | - |
Pyrococcus horikoshii | formyl-Ala + Ala-Ala-Ala-Ala-Ala | - |
? | |
3.4.19.1 | formyl-Met-Ala + H2O | - |
Pyrococcus horikoshii | formyl-Met + Ala | - |
? | |
3.4.19.1 | formyl-Met-Ala-Ala-Ala-Ala-Ala + H2O | - |
Pyrococcus horikoshii | formyl-Met + Ala-Ala-Ala-Ala-Ala | - |
? | |
3.4.19.1 | formyl-Met-Ala-Ser + H2O | - |
Pyrococcus horikoshii | formyl-Met + Ala-Ser | - |
? | |
3.4.19.1 | formyl-Met-Leu-Gly + H2O | - |
Pyrococcus horikoshii | formyl-Met + Leu-Gly | - |
? | |
3.4.19.1 | additional information | high hydrolytic activity for acylpeptides, no hydrolytic activity for Leu-4-nitroanilide and Ala-4-nitroanilide | Pyrococcus horikoshii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.19.1 | ? | x * 70000, SDS-PAGE | Pyrococcus horikoshii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.19.1 | AARE | - |
Pyrococcus horikoshii |
3.4.19.1 | acylamino acid-releasing enzyme | - |
Pyrococcus horikoshii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.19.1 | additional information | - |
the specific activity of the enzyme increases 714fold with heat treatment, suggesting the modification of the structure of the enzyme for optimal hydrolytic activity by heating | Pyrococcus horikoshii |
3.4.19.1 | 85 | - |
assay at | Pyrococcus horikoshii |
3.4.19.1 | 90 | - |
- |
Pyrococcus horikoshii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.19.1 | 90 | - |
stable for 24 h | Pyrococcus horikoshii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.19.1 | 17.3 | - |
acetyl-Ala-4-nitroanilide | pH 5.4, 85°C | Pyrococcus horikoshii | |
3.4.19.1 | 144 | - |
acetyl-Ala-4-nitroanilide | pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii | |
3.4.19.1 | 281 | - |
Acetyl-Ala-Ala-Ala-Ala | pH 5.4, 85°C | Pyrococcus horikoshii | |
3.4.19.1 | 330 | - |
acetyl-Leu-4-nitroanilide | pH 5.4, 85°C | Pyrococcus horikoshii | |
3.4.19.1 | 790 | - |
Acetyl-Ala-Ala | pH 5.4, 85°C | Pyrococcus horikoshii | |
3.4.19.1 | 2090 | - |
Acetyl-Ala-Ala-Ala-Ala | pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii | |
3.4.19.1 | 3200 | - |
acetyl-Leu-4-nitroanilide | pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii | |
3.4.19.1 | 9900 | - |
Acetyl-Ala-Ala | pH 5.4, 85°C, heat-activated enzyme | Pyrococcus horikoshii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.19.1 | 4.8 | 5.5 | at 85°C | Pyrococcus horikoshii |
3.4.19.1 | 5.4 | - |
assay at | Pyrococcus horikoshii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.19.1 | metabolism | the existence of the acylamino acid-releasing enzyme in archaea suggests that the mechanisms of protein degradation or initiation of protein synthesis or both in archaea may be similar to those in eukaryotes | Pyrococcus horikoshii |