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Literature summary extracted from

  • Yokochi, N.; Yoshikane, Y.; Matsumoto, S.; Fujisawa, M.; Ohnishi, K.; Yagi, T.
    Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase (2009), J. Biochem., 145, 493-503 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.42 E149Q mutant shows a showed a different pH optimum depending on the cosubstrate. With NAD+, the mutant shows very low activity with an optimum pH at 8.5 in the universal buffer. In contrast, the optimum pH is 5.5 with NADH Mesorhizobium loti
1.1.99.42 H137L almost complete loss of activity Mesorhizobium loti
1.2.1.100 E149Q mutant shows a showed a different pH optimum depending on the cosubstrate. With NAD+, the mutant shows very low activity with an optimum pH at 8.5 in the universal buffer. In contrast, the optimum pH is 5.5 with NADH Mesorhizobium loti
1.2.1.100 H137L almost complete loss of activity Mesorhizobium loti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.42 0.0124
-
 NADH wild-type, pH 8.0, 30°C Mesorhizobium loti
1.1.99.42 0.02
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate mutant E149Q, pH 8.0, 30°C Mesorhizobium loti
1.1.99.42 0.0249
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate wild-type, pH 8.0, 30°C Mesorhizobium loti
1.1.99.42 0.026
-
 NADH mutant E149Q, pH 8.0, 30°C Mesorhizobium loti
1.1.99.42 0.03
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate mutant E149Q, pH 5.0, 30°C Mesorhizobium loti
1.1.99.42 0.033
-
 NADH mutant E149Q, pH 5.0, 30°C Mesorhizobium loti
1.2.1.100 0.0058
-
 NAD+ mutant E149Q, pH 8.0, 30°C Mesorhizobium loti
1.2.1.100 0.0343
-
 NAD+ wild-type, pH 8.0, 30°C Mesorhizobium loti
1.2.1.100 0.0428
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate wild-type, pH 8.0, 30°C Mesorhizobium loti
1.2.1.100 0.241
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate mutant E149Q, pH 8.0, 30°C Mesorhizobium loti

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.42 59100
-
 gel filtration Mesorhizobium loti
1.2.1.100 59100
-
 gel filtration Mesorhizobium loti

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.42 Mesorhizobium loti Q988H5 enzyme additionally catalyzes the reaction of EC 1.2.1.100
-
1.1.99.42 Mesorhizobium loti MAFF303099 Q988H5 enzyme additionally catalyzes the reaction of EC 1.2.1.100
-
1.2.1.100 Mesorhizobium loti Q988H5 enzyme additionally catalyzes the reaction of 4-pyridoxic acid dehydrogenase
-
1.2.1.100 Mesorhizobium loti MAFF303099 Q988H5 enzyme additionally catalyzes the reaction of 4-pyridoxic acid dehydrogenase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.42 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+
-
 Mesorhizobium loti 4-pyridoxate + NAD+
-
 ?
1.1.99.42 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+
-
 Mesorhizobium loti MAFF303099 4-pyridoxate + NAD+
-
 ?
1.1.99.42 additional information enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, i.e. reaction of EC 1.2.1.100, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction Mesorhizobium loti ?
-
 ?
1.1.99.42 additional information enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, i.e. reaction of EC 1.2.1.100, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction Mesorhizobium loti MAFF303099 ?
-
 ?
1.2.1.100 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
-
 Mesorhizobium loti 3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
-
 ?
1.2.1.100 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
-
 Mesorhizobium loti MAFF303099 3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
-
 ?
1.2.1.100 additional information enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid, i.e. reaction of EC 4-pyridoxic acid dehydrogenase. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction Mesorhizobium loti ?
-
 ?
1.2.1.100 additional information enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid, i.e. reaction of EC 4-pyridoxic acid dehydrogenase. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction Mesorhizobium loti MAFF303099 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.42 dimer 2 * 38900, SDS-PAGE, 2 * 33060, calculated from sequence, 2 * 33121, TOF-MS Mesorhizobium loti
1.2.1.100 dimer 2 * 38900, SDS-PAGE, 2 * 33060, calculated from sequence, 2 * 33121, TOF-MS Mesorhizobium loti

Synonyms

EC Number Synonyms Comment Organism
1.1.99.42 mlr6739
-
 Mesorhizobium loti
1.2.1.100 mlr6739
-
 Mesorhizobium loti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.42 55
-
-
 Mesorhizobium loti
1.2.1.100 55
-
-
 Mesorhizobium loti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.42 78
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate mutant E149Q, pH 8.0, 30°C Mesorhizobium loti
1.1.99.42 217
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate wild-type, pH 8.0, 30°C Mesorhizobium loti
1.1.99.42 300
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate mutant E149Q, pH 5.0, 30°C Mesorhizobium loti
1.2.1.100 25
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate mutant E149Q, pH 8.0, 30°C Mesorhizobium loti
1.2.1.100 204
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate wild-type, pH 8.0, 30°C Mesorhizobium loti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.42 8
-
-
 Mesorhizobium loti
1.2.1.100 8
-
-
 Mesorhizobium loti

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.42 NADH
-
 Mesorhizobium loti

General Information

EC Number General Information Comment Organism
1.1.99.42 physiological function contrary to wild-type, mlr6793-disruptant cells cannot grow on pyridoxine, 4-pyridoxic acid or 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate as sole carbon and nitrogen source Mesorhizobium loti
1.2.1.100 physiological function contrary to wild-type, mlr6793-disruptant cells cannot grow on pyridoxine, 4-pyridoxic acid or 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate as sole carbon and nitrogen source Mesorhizobium loti