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Literature summary extracted from

  • Lee, C.R.; Chi, W.J.; Lim, J.H.; Dhakshnamoorthy, V.; Hong, S.K.
    Expression and characterization of the processive exo-beta-1,4-cellobiohydrolase SCO6546 from Streptomyces coelicolor A(3) (2018), J. Basic Microbiol., 58, 310-321 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.91 expressed in Streptomyces lividans strain TK24 Streptomyces coelicolor
3.2.1.91 expressed in Streptomyces lividans TK24 cells Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.91 Co2+ about 40% residual activity at 5 mM; the addition of 5 mM CoCl2 inhibits enzyme activity by 59% Streptomyces coelicolor
3.2.1.91 Cu2+ about 80% residual activity at 5 mM; the addition of 5 mM CuCl2 inhibits enzyme activity by 21% Streptomyces coelicolor
3.2.1.91 Mn2+ about 30% residual activity at 5 mM; the addition of 5 mM MnCl2 inhibits enzyme activity by 64% Streptomyces coelicolor
3.2.1.91 additional information EDTA (5 mM) does not affect the enzyme activity; the addition of EDTA (5 mM) does not affect enzyme activity Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.91 2.7
-
 cellotetraose at pH 5.0 and 50°C Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.91 Ca2+ the addition of CaCl2 enhances enzyme activity by 159% Streptomyces coelicolor
3.2.1.91 Ca2+ about 159% activity at 5 mM Streptomyces coelicolor
3.2.1.91 additional information the enzyme does not require a metal cofactor Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.91 cellulose + H2O Streptomyces coelicolor the enzyme acts on nonreducing end of cellulose cellobiose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Streptomyces coelicolor
-
-
-
3.2.1.91 Streptomyces coelicolor O86728
-
-
3.2.1.91 Streptomyces coelicolor A(3)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91 ammonium sulfate precipitation and Superdex 200HR gel filtration Streptomyces coelicolor
3.2.1.91 Superdex 200 gel filtration Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-nitrophenyl cellopentaoside + H2O
-
 Streptomyces coelicolor cellobiose + 4-nitrophenyl cellotrioside
-
 ?
3.2.1.91 4-nitrophenyl cellotetraoside + H2O
-
 Streptomyces coelicolor cellobiose + 4-nitrophenyl cellobioside
-
 ?
3.2.1.91 agarose + H2O low activity Streptomyces coelicolor ?
-
 ?
3.2.1.91 agarose + H2O about 40% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
3.2.1.91 avicel + H2O highest activity Streptomyces coelicolor cellobiose
-
 ?
3.2.1.91 avicel + H2O high activity Streptomyces coelicolor cellobiose
-
 ?
3.2.1.91 beechwood xylan + H2O low activity Streptomyces coelicolor ?
-
 ?
3.2.1.91 beechwood xylan + H2O about 20% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
3.2.1.91 beta-glucan + H2O low activity Streptomyces coelicolor ?
-
 ?
3.2.1.91 beta-glucan + H2O low activity Streptomyces coelicolor cellobiose
-
 ?
3.2.1.91 carboxymethyl cellulose + H2O high activity Streptomyces coelicolor cellobiose + ?
-
 ?
3.2.1.91 carboxymethyl cellulose + H2O 97% activity compared to Avicel Streptomyces coelicolor cellobiose
-
 ?
3.2.1.91 cellohexaose + H2O
-
 Streptomyces coelicolor cellobiose + cellotetraose + cellotriose
-
 ?
3.2.1.91 cellopentaose + H2O
-
 Streptomyces coelicolor cellobiose + cellotriose
-
 ?
3.2.1.91 cellotetraose + H2O
-
 Streptomyces coelicolor 2 cellobiose
-
 ?
3.2.1.91 cellotetraose + H2O
-
 Streptomyces coelicolor A(3) 2 cellobiose
-
 ?
3.2.1.91 cellulose + H2O the enzyme acts on nonreducing end of cellulose Streptomyces coelicolor cellobiose + ?
-
 ?
3.2.1.91 filter paper + H2O low activity Streptomyces coelicolor ?
-
 ?
3.2.1.91 filter paper + H2O about 18% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
3.2.1.91 galactan + H2O low activity Streptomyces coelicolor ?
-
 ?
3.2.1.91 galactan + H2O about 30% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
3.2.1.91 galactan + H2O about 30% activity compared to Avicel Streptomyces coelicolor A(3) ?
-
 ?
3.2.1.91 melibiose + H2O about 4% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
3.2.1.91 additional information the enzyme completely lacks chromogenic activity on the 4-nitrophenyl-cellodextrins from 4-nitrophenyl glucoside to 4-nitrophenyl cellobioside Streptomyces coelicolor ?
-
 ?
3.2.1.91 additional information no activity with cellotriose, alpha-cellulose, cellobiose, and raffinose. The enzyme completely lacks chromogenic activity on the 4-nitrophenyl cellodextrins from 4-nitrophenyl glucoside to 4-nitrophenyl cellobioside Streptomyces coelicolor ?
-
 ?
3.2.1.91 additional information no activity with cellotriose, alpha-cellulose, cellobiose, and raffinose. The enzyme completely lacks chromogenic activity on the 4-nitrophenyl cellodextrins from 4-nitrophenyl glucoside to 4-nitrophenyl cellobioside Streptomyces coelicolor A(3) ?
-
 ?
3.2.1.91 oat spelt xylan + H2O about 2% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
3.2.1.91 stachyose + H2O about 18% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
3.2.1.91 stachyose + H2O about 18% activity compared to Avicel Streptomyces coelicolor A(3) ?
-
 ?
3.2.1.91 xyloglucan + H2O low activity Streptomyces coelicolor ?
-
 ?
3.2.1.91 xyloglucan + H2O about 37% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
3.2.1.91 xyloglucan + H2O about 37% activity compared to Avicel Streptomyces coelicolor A(3) ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.91 ? x * 103400, calculated from amino acid sequence Streptomyces coelicolor
3.2.1.91 ? x * 99400, mature secreted protein, SDS-PAGE Streptomyces coelicolor
3.2.1.91 ? x * 99400, recombinant enzyme, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 cellobiohydrolase
-
 Streptomyces coelicolor
3.2.1.91 exo-beta-1,4-cellobiohydrolase
-
 Streptomyces coelicolor
3.2.1.91 SCO6546
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 50
-
-
 Streptomyces coelicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.91 30 60 the enzyme shows more than 55% of its maximum activity at 30-60 °C Streptomyces coelicolor
3.2.1.91 30 60 the enzyme retains more than 55% of its maximum activity at 30-60°C Streptomyces coelicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 30 60 the enzyme is stable up to 50°C, but is dramatically inactivated at 60°C (8% activity remaining after 1 h) Streptomyces coelicolor
3.2.1.91 50 60 the enzyme is stable up to 50°C, but is dramatically inactivated at 60°C (8% activity remaining after 1 h) Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.91 13.3
-
 cellotetraose at pH 5.0 and 50°C Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 5
-
-
 Streptomyces coelicolor
3.2.1.91 5
-
 pH optimum using Avicel or cellotetraose as substrates Streptomyces coelicolor
3.2.1.91 6
-
 pH optimum using cellopentaose as substrate Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.91 5 7 more than 50% activity between pH 5.0 and 7.0 Streptomyces coelicolor
3.2.1.91 5 7 more than 50% enzyme activity toward Avicel at pH 5.0-7.0 Streptomyces coelicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.91 4 9 the enzyme is stable over a wide pH range from 5.0-7.0, however, more than 75% of the cellulase activity is lost at pH 4.0 and 9.0 after 1 h Streptomyces coelicolor