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Literature summary extracted from

  • Kan, G.; Wen, H.; Wang, X.; Zhou, T.; Shi, C.
    Cloning and characterization of iron-superoxide dismutase in Antarctic yeast strain Rhodotorula mucilaginosa AN5 (2017), J. Basic Microbiol., 57, 680-690 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 gene RmFeSOD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3). Under copper stress experiment, the RmFeSOD-expressing Escherichia coli cells exhibit a better growth than nontransformed bacteria Rhodotorula mucilaginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 2-mercaptoethanol
-
 Rhodotorula mucilaginosa
1.15.1.1 DMSO
-
 Rhodotorula mucilaginosa
1.15.1.1 Mg2+ 30% inhibition at 1 mM Rhodotorula mucilaginosa
1.15.1.1 additional information no inhibition by DTT. Ba2+, Ca2+, Ni2+, and Fe2+ have no obvious impact to enzyme activity at 1 mM Rhodotorula mucilaginosa
1.15.1.1 PMSF
-
 Rhodotorula mucilaginosa
1.15.1.1 SDS 70% inhibition Rhodotorula mucilaginosa
1.15.1.1 Triton X-100 70% inhibition Rhodotorula mucilaginosa
1.15.1.1 Tween-80 70% inhibition Rhodotorula mucilaginosa
1.15.1.1 Urea
-
 Rhodotorula mucilaginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ activates the enzyme at 1 mM Rhodotorula mucilaginosa
1.15.1.1 Fe2+ required, a Fe-SOD Rhodotorula mucilaginosa
1.15.1.1 Fe3+ activates the enzyme at 1 mM Rhodotorula mucilaginosa
1.15.1.1 Mn2+ activates the enzyme at 1 mM Rhodotorula mucilaginosa
1.15.1.1 Zn2+ activates the enzyme at 1 mM Rhodotorula mucilaginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 49300
-
 recombinant His-tagged enzyme, gel filtration Rhodotorula mucilaginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+ Rhodotorula mucilaginosa
-
 O2 + H2O2
-
 ?
1.15.1.1 2 superoxide + 2 H+ Rhodotorula mucilaginosa AN5
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Rhodotorula mucilaginosa A0A1L5JYH9
-
-
1.15.1.1 Rhodotorula mucilaginosa AN5 A0A1L5JYH9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration Rhodotorula mucilaginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+
-
 Rhodotorula mucilaginosa O2 + H2O2
-
 ?
1.15.1.1 2 superoxide + 2 H+
-
 Rhodotorula mucilaginosa AN5 O2 + H2O2
-
 ?
1.15.1.1 additional information enzyme activity measurement by reduction of NBT Rhodotorula mucilaginosa ?
-
 ?
1.15.1.1 additional information enzyme activity measurement by reduction of NBT Rhodotorula mucilaginosa AN5 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 23500, about, sequence calculation, 2 * 31000, recombinant His-tagged enzyme, SDS-PAGE Rhodotorula mucilaginosa

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 iron SOD type
-
 Rhodotorula mucilaginosa
1.15.1.1 RmFeSOD
-
 Rhodotorula mucilaginosa
1.15.1.1 SOD
-
 Rhodotorula mucilaginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 25
-
 assay at Rhodotorula mucilaginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 20 40 recombinant enzyme, 1 h, about 90% activity remaining, completely stable for 45 min Rhodotorula mucilaginosa
1.15.1.1 50
-
 recombinant enzyme, 1 h, 80% activity remaining Rhodotorula mucilaginosa
1.15.1.1 60
-
 recombinant enzyme, 1 h, 60% activity remaining Rhodotorula mucilaginosa
1.15.1.1 70
-
 recombinant enzyme, 1 h, 20% activity remaining Rhodotorula mucilaginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 3 4
-
 Rhodotorula mucilaginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 1 11 activity range, 75% of maximal activity at pH 1.0, 45% at pH 11.0, profile overview Rhodotorula mucilaginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 1 9 recombinant RmFeSOD shows good pH stability in the pH 1.0-9.0 after 1 h incubation Rhodotorula mucilaginosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Rhodotorula mucilaginosa sequence calculation
-
 7.89

General Information

EC Number General Information Comment Organism
1.15.1.1 additional information the conserved histidine residues (His41, His89, His179), aspartate residue (Asp175), and WEHAYY domain play an important role in the active site of the Fe-binding sites Rhodotorula mucilaginosa
1.15.1.1 physiological function enzyme RmFeSOD plays an important role in the adaptability of heavy metals Rhodotorula mucilaginosa