BRENDA - Enzyme Database

Two unrelated 8-vinyl reductases ensure production of mature chlorophylls in Acaryochloris marina

Chen, G.E.; Hitchcock, A.; Jackson, P.J.; Chaudhuri, R.R.; Dickman, M.J.; Hunter, C.N.; Canniffe, D.P.; J. Bacteriol. 198, 1393-1400 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.3.1.75
gene bciA or nmrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, nmrA is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109
Acaryochloris marina
1.3.7.13
gene bciB or frhB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, frhB is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109
Acaryochloris marina
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.1.75
3,8-divinyl chlorophyllide a + NADPH + H+
Acaryochloris marina
-
chlorophyllide a + NADP+
-
-
?
1.3.1.75
3,8-divinyl chlorophyllide a + NADPH + H+
Acaryochloris marina MBIC11017
-
chlorophyllide a + NADP+
-
-
?
1.3.7.13
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
Acaryochloris marina
-
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
?
1.3.7.13
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
Acaryochloris marina MBIC11017
-
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.1.75
Acaryochloris marina
B0C357
-
-
1.3.1.75
Acaryochloris marina MBIC11017
B0C357
-
-
1.3.7.13
Acaryochloris marina
B0CAB4
-
-
1.3.7.13
Acaryochloris marina MBIC11017
B0CAB4
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.75
3,8-divinyl chlorophyllide a + NADPH + H+
-
745221
Acaryochloris marina
chlorophyllide a + NADP+
-
-
-
?
1.3.1.75
3,8-divinyl chlorophyllide a + NADPH + H+
-
745221
Acaryochloris marina MBIC11017
chlorophyllide a + NADP+
-
-
-
?
1.3.7.13
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
-
745221
Acaryochloris marina
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
-
?
1.3.7.13
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
-
745221
Acaryochloris marina MBIC11017
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.1.75
?
x * 36780, sequence calculation
Acaryochloris marina
1.3.7.13
?
x * 45492, sequence calculation
Acaryochloris marina
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.75
NADPH
-
Acaryochloris marina
1.3.7.13
ferredoxin [iron-sulfur] cluster
-
Acaryochloris marina
1.3.7.13
NADPH
-
Acaryochloris marina
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.75
gene bciA or nmrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, nmrA is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109
Acaryochloris marina
1.3.7.13
gene bciB or frhB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, frhB is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109
Acaryochloris marina
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.75
NADPH
-
Acaryochloris marina
1.3.7.13
ferredoxin [iron-sulfur] cluster
-
Acaryochloris marina
1.3.7.13
NADPH
-
Acaryochloris marina
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.1.75
3,8-divinyl chlorophyllide a + NADPH + H+
Acaryochloris marina
-
chlorophyllide a + NADP+
-
-
?
1.3.1.75
3,8-divinyl chlorophyllide a + NADPH + H+
Acaryochloris marina MBIC11017
-
chlorophyllide a + NADP+
-
-
?
1.3.7.13
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
Acaryochloris marina
-
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
?
1.3.7.13
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
Acaryochloris marina MBIC11017
-
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.75
3,8-divinyl chlorophyllide a + NADPH + H+
-
745221
Acaryochloris marina
chlorophyllide a + NADP+
-
-
-
?
1.3.1.75
3,8-divinyl chlorophyllide a + NADPH + H+
-
745221
Acaryochloris marina MBIC11017
chlorophyllide a + NADP+
-
-
-
?
1.3.7.13
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
-
745221
Acaryochloris marina
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
-
?
1.3.7.13
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
-
745221
Acaryochloris marina MBIC11017
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.1.75
?
x * 36780, sequence calculation
Acaryochloris marina
1.3.7.13
?
x * 45492, sequence calculation
Acaryochloris marina
General Information
EC Number
General Information
Commentary
Organism
1.3.1.75
evolution
two unrelated classes of 8-vinyl reductases are known to exist in oxygenic phototrophs, BciA and BciB. Transcript and proteomic analysis of Acaryochloris marina reveal that both bciA and bciB are expressed and their encoded proteins are present in the cell, possibly in order to ensure that all synthesized chlorophyll pigment carries an ethyl group at C-8. The presence of two 8-vinyl reductases is unique for cyanobacteria. The phylogenetic positions of Acaryochloris marina BciA and BciB are both broadly consistent with those shown for Acaryochloris marina in the 16S rRNA trees, suggesting that the bciA and bciB genes have not been acquired by horizontal transfer. However, the positions of Synechococcus spp. in the BciA tree and the clade containing the green sulfur bacteria in the BciB tree are inconsistent with the 16S rRNA phylogeny, indicating that there may have been lateral transfer events during the evolution of both bciA and bciB
Acaryochloris marina
1.3.1.75
malfunction
loss of 8-vinyl reductase activity in Acaryochloris marina results in the production of 8-vinyl-Chl a and 8-vinyl-Chl d with a negative effect on viability of the cells
Acaryochloris marina
1.3.1.75
additional information
the enzyme encoded by gene nmrA shows 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH) activity and shall be renamed bciA
Acaryochloris marina
1.3.1.75
physiological function
functional complementation of an enzyme-deficient DELTAbciB Synechocystis sp. strain PCC 6803
Acaryochloris marina
1.3.7.13
evolution
two unrelated classes of 8-vinyl reductases are known to exist in oxygenic phototrophs, BciA and BciB. Transcript and proteomic analysis of Acaryochloris marina reveal that both bciA and bciB are expressed and their encoded proteins are present in the cell, possibly in order to ensure that all synthesized chlorophyll pigment carries an ethyl group at C-8. The presence of two 8-vinyl reductases is unique for cyanobacteria. The phylogenetic positions of Acaryochloris marina BciA and BciB are both broadly consistent with those shown for Acaryochloris marina in the 16S rRNA trees, suggesting that the bciA and bciB genes have not been acquired by horizontal transfer. However, the positions of Synechococcus spp. in the BciA tree and the clade containing the green sulfur bacteria in the BciB tree are inconsistent with the 16S rRNA phylogeny, indicating that there may have been lateral transfer events during the evolution of both bciA and bciB
Acaryochloris marina
1.3.7.13
malfunction
loss of 8-vinyl reductase activity in Acaryochloris marina results in the production of 8-vinyl-Chl a and 8-vinyl-Chl d with a negative effect on viability of the cells
Acaryochloris marina
1.3.7.13
additional information
the enzyme encoded by gene frhB shows 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin) activity and shall be renamed bciB
Acaryochloris marina
1.3.7.13
physiological function
functional complementation of an enzyme-deficient DELTAbciB Synechocystis sp. strain PCC 6803
Acaryochloris marina
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.3.1.75
evolution
two unrelated classes of 8-vinyl reductases are known to exist in oxygenic phototrophs, BciA and BciB. Transcript and proteomic analysis of Acaryochloris marina reveal that both bciA and bciB are expressed and their encoded proteins are present in the cell, possibly in order to ensure that all synthesized chlorophyll pigment carries an ethyl group at C-8. The presence of two 8-vinyl reductases is unique for cyanobacteria. The phylogenetic positions of Acaryochloris marina BciA and BciB are both broadly consistent with those shown for Acaryochloris marina in the 16S rRNA trees, suggesting that the bciA and bciB genes have not been acquired by horizontal transfer. However, the positions of Synechococcus spp. in the BciA tree and the clade containing the green sulfur bacteria in the BciB tree are inconsistent with the 16S rRNA phylogeny, indicating that there may have been lateral transfer events during the evolution of both bciA and bciB
Acaryochloris marina
1.3.1.75
malfunction
loss of 8-vinyl reductase activity in Acaryochloris marina results in the production of 8-vinyl-Chl a and 8-vinyl-Chl d with a negative effect on viability of the cells
Acaryochloris marina
1.3.1.75
additional information
the enzyme encoded by gene nmrA shows 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH) activity and shall be renamed bciA
Acaryochloris marina
1.3.1.75
physiological function
functional complementation of an enzyme-deficient DELTAbciB Synechocystis sp. strain PCC 6803
Acaryochloris marina
1.3.7.13
evolution
two unrelated classes of 8-vinyl reductases are known to exist in oxygenic phototrophs, BciA and BciB. Transcript and proteomic analysis of Acaryochloris marina reveal that both bciA and bciB are expressed and their encoded proteins are present in the cell, possibly in order to ensure that all synthesized chlorophyll pigment carries an ethyl group at C-8. The presence of two 8-vinyl reductases is unique for cyanobacteria. The phylogenetic positions of Acaryochloris marina BciA and BciB are both broadly consistent with those shown for Acaryochloris marina in the 16S rRNA trees, suggesting that the bciA and bciB genes have not been acquired by horizontal transfer. However, the positions of Synechococcus spp. in the BciA tree and the clade containing the green sulfur bacteria in the BciB tree are inconsistent with the 16S rRNA phylogeny, indicating that there may have been lateral transfer events during the evolution of both bciA and bciB
Acaryochloris marina
1.3.7.13
malfunction
loss of 8-vinyl reductase activity in Acaryochloris marina results in the production of 8-vinyl-Chl a and 8-vinyl-Chl d with a negative effect on viability of the cells
Acaryochloris marina
1.3.7.13
additional information
the enzyme encoded by gene frhB shows 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin) activity and shall be renamed bciB
Acaryochloris marina
1.3.7.13
physiological function
functional complementation of an enzyme-deficient DELTAbciB Synechocystis sp. strain PCC 6803
Acaryochloris marina