BRENDA - Enzyme Database

Characterization of a bifunctional archaeal acyl coenzyme A carboxylase

Chuakrut, S.; Arai, H.; Ishii, M.; Igarashi, Y.; J. Bacteriol. 185, 938-947 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.4.1.2
additional information
NADP+, NADPH, 3-hydroxypropionate, succinate, fumarate, malate, and glyoxylate have no significant effect on acetyl-CoA carboxylase or propionyl-CoA carboxylase activities
Acidianus brierleyi
6.4.1.2
palmitoyl-CoA
0.5 mM palmitoyl-CoA has a slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%) activities
Acidianus brierleyi
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.2
ATP
an excess of ATP (2.0 mM) has an inhibitory effect on the enzymatic activities
Acidianus brierleyi
6.4.1.2
citrate
citrate inhibits the enzyme activity by more than 50% at concentrations of 3 mM
Acidianus brierleyi
6.4.1.2
Co2+
26% residual activity at 10-15 mM
Acidianus brierleyi
6.4.1.2
CoASH
inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13%
Acidianus brierleyi
6.4.1.2
dithiothreitol
dithiothreitol inhibits the enzyme slightly, by approximately 12%, at a final concentration of 5 to 10 mM
Acidianus brierleyi
6.4.1.2
K+
the enzyme shows less than 50% activity when the concentration is higher than 40 mM
Acidianus brierleyi
6.4.1.2
malonyl-CoA
inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13%
Acidianus brierleyi
6.4.1.2
methylmalonyl-CoA
60% inhibition at 0.5 mM; 79% inhibition at 0.5 mM
Acidianus brierleyi
6.4.1.2
Mg2+
an excess of Mg2+ (higher than 4.0 mM) has an inhibitory effect on the enzymatic activities
Acidianus brierleyi
6.4.1.2
Mn2+
an excess of Mn2+ (higher than 2.0 mM) has an inhibitory effect on the enzymatic activities
Acidianus brierleyi
6.4.1.2
Na+
the enzyme shows less than 50% activity when the concentration is higher than 40 mM
Acidianus brierleyi
6.4.1.2
succinyl-CoA
57% inhibition at 0.5 mM; 84% inhibition at 0.5 mM
Acidianus brierleyi
6.4.1.2
sulfate
sulfate inhibits the enzyme activity by more than 50% at concentrations of 25 mM
Acidianus brierleyi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.2
0.04
-
ATP
with acetyl-CoA as cosubstrate, at pH 7.5 and 70°C
Acidianus brierleyi
6.4.1.2
0.05
-
ATP
with propionyl-CoA as cosubstrate, at pH 7.5 and 70°C
Acidianus brierleyi
6.4.1.2
0.1
-
propionyl-CoA
at pH 7.5 and 70°C
Acidianus brierleyi
6.4.1.2
0.17
-
acetyl-CoA
at pH 7.5 and 70°C
Acidianus brierleyi
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.2
Mg2+
required, maximum activity at 4 mM
Acidianus brierleyi
6.4.1.2
additional information
Fe2+, Cu2+, Ca2+, and Ni2+ (0.5 to 20 mM) have no effect on enzyme activity
Acidianus brierleyi
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.2
540000
-
gel filtration
Acidianus brierleyi
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.4.1.2
ATP + acetyl-CoA + HCO3-
Acidianus brierleyi
-
ADP + phosphate + malonyl-CoA
-
-
?
6.4.1.2
ATP + propionyl-CoA + HCO3-
Acidianus brierleyi
-
ADP + phosphate + methylmalonyl-CoA
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.4.1.2
Acidianus brierleyi
Q877I4 and Q877I5 and Q877I3
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.4.1.2
Streptavidin column chromatography and Superose 6 gel filtration
Acidianus brierleyi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
745205
Acidianus brierleyi
ADP + phosphate + malonyl-CoA
-
-
-
?
6.4.1.2
ATP + propionyl-CoA + HCO3-
-
745205
Acidianus brierleyi
ADP + phosphate + methylmalonyl-CoA
-
-
-
?
6.4.1.2
additional information
neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme
745205
Acidianus brierleyi
?
-
-
-
?
6.4.1.2
n-butyryl-CoA + HCO3-
4.6% activity compared to acetyl-CoA or propionyl-CoA
745205
Acidianus brierleyi
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.4.1.2
homotrimer
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
Synonyms
EC Number
Synonyms
Commentary
Organism
6.4.1.2
ACCB
-
Acidianus brierleyi
6.4.1.2
AccC
-
Acidianus brierleyi
6.4.1.2
acyl coenzyme A carboxylase
-
Acidianus brierleyi
6.4.1.2
acyl-CoA carboxylase
-
Acidianus brierleyi
6.4.1.2
PccB
-
Acidianus brierleyi
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.4.1.2
60
70
-
Acidianus brierleyi
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.4.1.2
50
90
the activity of the enzyme gradually decreases at 50°C, with a residual activity of around 60% after 2 h of incubation. At 60°C, the residual activity of the enzyme is 50% after incubation for 35 to 40 min, whereas the residual activities at 70 and 80°C are less than 15%. The enzyme completely loses its activity within 10 min when it is incubated at 90°C or above
Acidianus brierleyi
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4.1.2
6.4
6.9
-
Acidianus brierleyi
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.2
ATP
the enzyme exhibits the maximum activity when the ATP concentration is 1.0-1.5 mM. Neither ADP, AMP, GTP, CTP, UTP, nor dTTP (0.2 mM) can substitute for ATP
Acidianus brierleyi
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.4.1.2
additional information
NADP+, NADPH, 3-hydroxypropionate, succinate, fumarate, malate, and glyoxylate have no significant effect on acetyl-CoA carboxylase or propionyl-CoA carboxylase activities
Acidianus brierleyi
6.4.1.2
palmitoyl-CoA
0.5 mM palmitoyl-CoA has a slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%) activities
Acidianus brierleyi
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.2
ATP
the enzyme exhibits the maximum activity when the ATP concentration is 1.0-1.5 mM. Neither ADP, AMP, GTP, CTP, UTP, nor dTTP (0.2 mM) can substitute for ATP
Acidianus brierleyi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.2
ATP
an excess of ATP (2.0 mM) has an inhibitory effect on the enzymatic activities
Acidianus brierleyi
6.4.1.2
citrate
citrate inhibits the enzyme activity by more than 50% at concentrations of 3 mM
Acidianus brierleyi
6.4.1.2
Co2+
26% residual activity at 10-15 mM
Acidianus brierleyi
6.4.1.2
CoASH
inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13%
Acidianus brierleyi
6.4.1.2
dithiothreitol
dithiothreitol inhibits the enzyme slightly, by approximately 12%, at a final concentration of 5 to 10 mM
Acidianus brierleyi
6.4.1.2
K+
the enzyme shows less than 50% activity when the concentration is higher than 40 mM
Acidianus brierleyi
6.4.1.2
malonyl-CoA
inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13%
Acidianus brierleyi
6.4.1.2
methylmalonyl-CoA
60% inhibition at 0.5 mM
Acidianus brierleyi
6.4.1.2
methylmalonyl-CoA
79% inhibition at 0.5 mM
Acidianus brierleyi
6.4.1.2
Mg2+
an excess of Mg2+ (higher than 4.0 mM) has an inhibitory effect on the enzymatic activities
Acidianus brierleyi
6.4.1.2
Mn2+
an excess of Mn2+ (higher than 2.0 mM) has an inhibitory effect on the enzymatic activities
Acidianus brierleyi
6.4.1.2
Na+
the enzyme shows less than 50% activity when the concentration is higher than 40 mM
Acidianus brierleyi
6.4.1.2
succinyl-CoA
57% inhibition at 0.5 mM
Acidianus brierleyi
6.4.1.2
succinyl-CoA
84% inhibition at 0.5 mM
Acidianus brierleyi
6.4.1.2
sulfate
sulfate inhibits the enzyme activity by more than 50% at concentrations of 25 mM
Acidianus brierleyi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.2
0.04
-
ATP
with acetyl-CoA as cosubstrate, at pH 7.5 and 70°C
Acidianus brierleyi
6.4.1.2
0.05
-
ATP
with propionyl-CoA as cosubstrate, at pH 7.5 and 70°C
Acidianus brierleyi
6.4.1.2
0.1
-
propionyl-CoA
at pH 7.5 and 70°C
Acidianus brierleyi
6.4.1.2
0.17
-
acetyl-CoA
at pH 7.5 and 70°C
Acidianus brierleyi
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.2
Mg2+
required, maximum activity at 4 mM
Acidianus brierleyi
6.4.1.2
additional information
Fe2+, Cu2+, Ca2+, and Ni2+ (0.5 to 20 mM) have no effect on enzyme activity
Acidianus brierleyi
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.2
540000
-
gel filtration
Acidianus brierleyi
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.4.1.2
ATP + acetyl-CoA + HCO3-
Acidianus brierleyi
-
ADP + phosphate + malonyl-CoA
-
-
?
6.4.1.2
ATP + propionyl-CoA + HCO3-
Acidianus brierleyi
-
ADP + phosphate + methylmalonyl-CoA
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.4.1.2
Streptavidin column chromatography and Superose 6 gel filtration
Acidianus brierleyi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
745205
Acidianus brierleyi
ADP + phosphate + malonyl-CoA
-
-
-
?
6.4.1.2
ATP + propionyl-CoA + HCO3-
-
745205
Acidianus brierleyi
ADP + phosphate + methylmalonyl-CoA
-
-
-
?
6.4.1.2
additional information
neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme
745205
Acidianus brierleyi
?
-
-
-
?
6.4.1.2
n-butyryl-CoA + HCO3-
4.6% activity compared to acetyl-CoA or propionyl-CoA
745205
Acidianus brierleyi
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.4.1.2
homotrimer
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.4.1.2
60
70
-
Acidianus brierleyi
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.4.1.2
50
90
the activity of the enzyme gradually decreases at 50°C, with a residual activity of around 60% after 2 h of incubation. At 60°C, the residual activity of the enzyme is 50% after incubation for 35 to 40 min, whereas the residual activities at 70 and 80°C are less than 15%. The enzyme completely loses its activity within 10 min when it is incubated at 90°C or above
Acidianus brierleyi
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4.1.2
6.4
6.9
-
Acidianus brierleyi