BRENDA - Enzyme Database

p-Cymene catabolic pathway in Pseudomonas putida F1 cloning and characterization of DNA encoding conversion of p-cymene to p-cumate

Eaton, R.W.; J. Bacteriol. 179, 3171-3180 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.25
expression in Escherichia coli
Pseudomonas putida
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.25
Pseudomonas putida
O33456 and O33457
O33456 i.e. hydroxylase subunit CymAa, O33457 i.e. reductase subunit CymAb, cf. EC 1.8.1.3
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.25
additional information
enzyme can attack benzylic and toluic alcohols as well as toluene and xylenes, probably with the formation of gem-diol intermediates which spontaneously dehydrate to yield the corresponding aldehydes
745198
Pseudomonas putida
?
-
-
-
-
1.14.15.25
p-cymene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
745198
Pseudomonas putida
4-isopropylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.15.25
?
x * 43117, hydrolase subunit CymAa, x * 38295, reductase subunit CymAb, calculated
Pseudomonas putida
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.25
Ferredoxin
reductase subunit CymAb
Pseudomonas putida
1.14.15.25
[2Fe-2S]-center
reductase subunit CymAb
Pseudomonas putida
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.25
expression in Escherichia coli
Pseudomonas putida
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.25
Ferredoxin
reductase subunit CymAb
Pseudomonas putida
1.14.15.25
[2Fe-2S]-center
reductase subunit CymAb
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.25
additional information
enzyme can attack benzylic and toluic alcohols as well as toluene and xylenes, probably with the formation of gem-diol intermediates which spontaneously dehydrate to yield the corresponding aldehydes
745198
Pseudomonas putida
?
-
-
-
-
1.14.15.25
p-cymene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
745198
Pseudomonas putida
4-isopropylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.15.25
?
x * 43117, hydrolase subunit CymAa, x * 38295, reductase subunit CymAb, calculated
Pseudomonas putida