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Literature summary extracted from

  • Lee, J.; Seo, G.; An, S.; Kim, H.
    A cold-active acidophilic endoglucanase of Paenibacillus sp. Y2 isolated from soil in an alpine region (2017), J. Appl. Biol. Chem., 60, 257-263 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 Cu2+ 5 mM, 136% of initial activity Paenibacillus sp. Y2
3.2.1.4 Mg2+ 5 mM, 127% of initial activity Paenibacillus sp. Y2
3.2.1.4 NaCl 0.5 M, 211% of initial activity Paenibacillus sp. Y2

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Co2+ 5 mM, 43% of initial activity Paenibacillus sp. Y2
3.2.1.4 EDTA 5 mM, 63% of initial activity Paenibacillus sp. Y2
3.2.1.4 Fe2+ 5 mM, 77% of initial activity Paenibacillus sp. Y2
3.2.1.4 Mn2+ 5 mM, 60% of initial activity Paenibacillus sp. Y2
3.2.1.4 SDS 1%, 12% of initial activity Paenibacillus sp. Y2
3.2.1.4 Zn2+ 5 mM, 22% of initial activity Paenibacillus sp. Y2

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular
-
 Paenibacillus sp. Y2
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Paenibacillus sp. Y2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Paenibacillus sp. Y2

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 6.52
-
 pH 4.5, 30°C Paenibacillus sp. Y2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 barley beta-glucan + H2O
-
 Paenibacillus sp. Y2 ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Paenibacillus sp. Y2 ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Paenibacillus sp. Y2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 38000, SDS-PAGE Paenibacillus sp. Y2

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 30
-
-
 Paenibacillus sp. Y2

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 20
-
 79% of maximum activity Paenibacillus sp. Y2
3.2.1.4 50
-
 90% of maximum activity Paenibacillus sp. Y2

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 50
-
 half-life 140 min Paenibacillus sp. Y2
3.2.1.4 55
-
 5 min, loss of activity Paenibacillus sp. Y2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4.5
-
-
 Paenibacillus sp. Y2

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4 5.5 more than 60% of maximum activity Paenibacillus sp. Y2