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Literature summary extracted from

  • Feng, V.; Joh, Y.; Ishikawa, K.; Ishida, H.; Ando, S.; Yamagaki, T.; Nakanishi, H.; Cao, S.; Matsui, I.; Kosugi, Y.
    Thermophilic phospholipase A2 in the cytosolic fraction from the archaeon Pyrococcus horikoshii (2000), J. Am. Oil Chem. Soc., 77, 1147-1152 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.4 glycerol 3-20% (v/v), activity is enhanced more than 5-18fold Pyrococcus horikoshii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 Ca2+ activity is unaffected by the addition of 0.0001-1 mM calcium, but is inhibited slightly by the addition of 2-10 mM calcium Pyrococcus horikoshii
3.1.1.4 additional information activity is unaffected by the addition of 1-5 mM EDTA or 0.01-20 mM dithiothreitol Pyrococcus horikoshii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 cytosol
-
 Pyrococcus horikoshii 5829
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Pyrococcus horikoshii
-
-
-
3.1.1.4 Pyrococcus horikoshii OT-3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 partial, the phospholipase A2 is difficult to purify by general chromatography because of aggregation Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-hexadecanoyl-2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminohexanoyl]-sn-glycero-3-phosphocholine + H2O sensitive fluorescent substrate that is stable at 95°C Pyrococcus horikoshii 1-hexadecanoyl-sn-glycero-3-phosphocholine + 6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoic acid
-
 ?
3.1.1.4 1-hexadecanoyl-2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminohexanoyl]-sn-glycero-3-phosphocholine + H2O sensitive fluorescent substrate that is stable at 95°C Pyrococcus horikoshii OT-3 1-hexadecanoyl-sn-glycero-3-phosphocholine + 6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoic acid
-
 ?
3.1.1.4 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii 1-palmitoyl-sn-glycero-3-phosphocholine + arachidonate
-
 ?
3.1.1.4 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii OT-3 1-palmitoyl-sn-glycero-3-phosphocholine + arachidonate
-
 ?
3.1.1.4 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii 1-palmitoyl-sn-glycero-3-phosphoethanolamine + arachidonate
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii 1-palmitoyl-sn-glycero-3-phosphocholine + linoleate
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii OT-3 1-palmitoyl-sn-glycero-3-phosphocholine + linoleate
-
 ?
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii 1-palmitoyl-sn-glycero-3-phosphocholine + oleate
-
 ?
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii OT-3 1-palmitoyl-sn-glycero-3-phosphocholine + oleate
-
 ?
3.1.1.4 additional information no fatty acid is produced by the cell lysate when trimyristin, tristearin, or triolein is incubated at 90°C overnight. Therefore, no thermostable lipase activity is observed in the PLA2 from Pyrococcus horikoshii Pyrococcus horikoshii ?
-
 ?
3.1.1.4 additional information no fatty acid is produced by the cell lysate when trimyristin, tristearin, or triolein is incubated at 90°C overnight. Therefore, no thermostable lipase activity is observed in the PLA2 from Pyrococcus horikoshii Pyrococcus horikoshii OT-3 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 PLA1
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.4 90
-
-
 Pyrococcus horikoshii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.4 80 95 80°C: about 80% of maximal activity, 95°C: about 20% of maximal activity Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.4 80
-
 the enzyme retains more than 95% activity for 80 min at pH 7.5 Pyrococcus horikoshii
3.1.1.4 90
-
 the enzyme retains more than 85% activity for 80 min at pH 7.5 Pyrococcus horikoshii
3.1.1.4 100
-
 80 min, pH 7.5, 65% inactivation Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 7
-
-
 Pyrococcus horikoshii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.4 6.5 7.5 pH 6.5: about 50% of maximal acfivity, pH 7.5: 75% of maximal activity Pyrococcus horikoshii