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Literature summary extracted from

  • Song, J.M.; Hong, S.K.; An, Y.J.; Kang, M.H.; Hong, K.H.; Lee, Y.H.; Cha, S.S.
    Genetic and structural characterization of a thermo-tolerant, cold-active, and acidic endo-beta-1,4-glucanase from Antarctic springtail, Cryptopygus antarcticus (2017), J. Agric. Food Chem., 65, 1630-1640 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 degradation under acidic conditions at 50°C, the enzyme is effective in digesting the green algae Ulva pertusa Cryptopygus antarcticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Cryptopygus antarcticus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 molecular modeling of structure Cryptopygus antarcticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Cryptopygus antarcticus D3GDK4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 13.9
-
 substrate barley beta-glucan, pH 3.5, 40°C Cryptopygus antarcticus
3.2.1.4 20.5
-
 substrate lichenan, pH 3.5, 40°C Cryptopygus antarcticus
3.2.1.4 35.8
-
 substrate carboxymethylcellulose, pH 3.5, 40°C Cryptopygus antarcticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 barley beta-glucan + H2O
-
 Cryptopygus antarcticus ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Cryptopygus antarcticus cellobiose + cellotriose + cellotetraose
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Cryptopygus antarcticus cellotetraose + cellobiose
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Cryptopygus antarcticus cellotriose + cellobiose
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Cryptopygus antarcticus 2 cellobiose
-
 ?
3.2.1.4 lichenan + H2O
-
 Cryptopygus antarcticus ?
-
 ?
3.2.1.4 additional information no substrates: cellobiose and cellotriose Cryptopygus antarcticus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 40
-
-
 Cryptopygus antarcticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4
-
 10 60% of maximum activity Cryptopygus antarcticus
3.2.1.4 80
-
 40% of maximum activity Cryptopygus antarcticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 60
-
 8 h, no loss of activity Cryptopygus antarcticus
3.2.1.4 70
-
 half-life 4 h Cryptopygus antarcticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 3.5
-
-
 Cryptopygus antarcticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 2 7 more than 60% of maximum activity Cryptopygus antarcticus