EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.18.1 | drug development | the regulation of melanin synthesis via the inhibition of tyrosinase is a research topic in the context of preventing hyperpigmentation, thus tyrosinase inhibitors are increasingly important in the food, medical, and cosmetic industries. The evaluated suface plasmon resonance biosensor has the potential to be used in the detection and screening of inhibitor drug candidates | Agaricus bisporus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | catechol | enzyme-inhibitor interaction measurement by SPR | Agaricus bisporus | |
1.14.18.1 | additional information | inhibitor screening using surface plasmon resonance (SPR), two-state modeling. Structural changes of the mushroom tyrosinase in the presence of inhibitors are analyzed by circular dichroism spectroscopy | Agaricus bisporus | |
1.14.18.1 | phloroglucinol | enzyme-inhibitor interaction measurement by SPR | Agaricus bisporus | |
1.14.18.1 | pyrogallol | enzyme-inhibitor interaction measurement by SPR | Agaricus bisporus | |
1.14.18.1 | saffron | enzyme-inhibitor interaction measurement by SPR | Agaricus bisporus | |
1.14.18.1 | Tannic acid | enzyme-inhibitor interaction measurement by SPR | Agaricus bisporus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | 2 L-dopa + O2 | Agaricus bisporus | - |
2 dopaquinone + 2 H2O | - |
? | |
1.14.18.1 | L-tyrosine + O2 | Agaricus bisporus | - |
dopaquinone + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.1 | Agaricus bisporus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.18.1 | commercial preparation | - |
Agaricus bisporus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | 2 L-dopa + O2 | - |
Agaricus bisporus | 2 dopaquinone + 2 H2O | - |
? | |
1.14.18.1 | L-tyrosine + O2 | - |
Agaricus bisporus | dopaquinone + H2O | - |
? | |
1.14.18.1 | additional information | monophenolase and diphenolase activities of mushroom tyrosinase are performed using L-tyrosine and L-DOPA, respectively, by measuring the dopachrome accumulation at 475 nm before immobilization on the chip surface for surface plasmon resonance analysis | Agaricus bisporus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.18.1 | mushroom tyrosinase | - |
Agaricus bisporus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | additional information | - |
additional information | inhibition kinetics | Agaricus bisporus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.18.1 | physiological function | tyrosinase is a multifunctional copper-containing oxygenase and catalyzes the hydroxylation of a monophenol and the conversion of o-diphenols to the corresponding o-quinones. These quinones are highly reactive compounds that can polymerize spontaneously to form melanin. Products formed as a result of tyrosinase activity are precursors for skin pigmentation, defense, and protective mechanisms in fungi | Agaricus bisporus |