Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vargas, D.; Hageman, S.; Gulati, M.; Nobile, C.J.; Rawat, M.
    S-nitrosomycothiol reductase and mycothiol are required for survival under aldehyde stress and biofilm formation in Mycobacterium smegmatis (2016), IUBMB Life, 68, 621-628 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.13 gene mshC, enzyme expression analysis Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.13 additional information generation of mshC transposon disruption mutants, with or without co-disruption of gene mscR Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.13 Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.13 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP Mycolicibacterium smegmatis
-
 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate
-
 ?
6.3.1.13 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP Mycolicibacterium smegmatis mc2155
-
 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.13 Mycolicibacterium smegmatis A0QZY0
-
-
6.3.1.13 Mycolicibacterium smegmatis mc2155 A0QZY0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.13 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP
-
 Mycolicibacterium smegmatis 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate
-
 ?
6.3.1.13 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP
-
 Mycolicibacterium smegmatis mc2155 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.13 MSH ligase
-
 Mycolicibacterium smegmatis
6.3.1.13 MshC
-
 Mycolicibacterium smegmatis
6.3.1.13 mycothiol ligase
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.13 ATP
-
 Mycolicibacterium smegmatis

Expression

EC Number Organism Comment Expression
6.3.1.13 Mycolicibacterium smegmatis gene mshC is induced by S-nitrosoglutathione (GSNO) and aldehydes. Gene mshC expression increases under aldehyde stress and nitrosative stress up

General Information

EC Number General Information Comment Organism
6.3.1.13 malfunction Mycobacterium smegmatis mutants disrupted in mscR, coding for a dual function S-nitrosomycothiol reductase and formaldehyde dehydrogenase, and mshC, coding for a mycothiol ligase, EC 6.3.1.13, and lacking mycothiol (MSH), are more susceptible to S-nitrosoglutathione (GSNO) and aldehydes than wild-type. MSH is a cofactor for MscR, and both mshC and mscR are induced by GSNO and aldehydes. The transposon mutant, S24, disrupted in mshC, is most sensitive to killing by GSNO Mycolicibacterium smegmatis
6.3.1.13 metabolism the enzyme catalyzes the fourth step of a five step mycothiol, MSH, biosynthetic pathway Mycolicibacterium smegmatis
6.3.1.13 physiological function mycothiol and S-nitrosomycothiol reductase are required for normal biofilm formation in Mycobacterium smegmatis Mycolicibacterium smegmatis