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Literature summary extracted from

  • Lin, S.; Zhang, G.; Liao, Y.; Pan, J.
    Inhibition of chrysin on xanthine oxidase activity and its inhibition mechanism (2015), Int. J. Biol. Macromol., 81, 274-282 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.3.2 allopurinol
-
 Bos taurus
1.17.3.2 apigenin binding structure, thermodynamic, and kinetic analysis, computational docking Bos taurus
1.17.3.2 chrysin strong reversible competitive inhibition, inhibition mechanism. Chrysin interacts with the amino acid residues Leu648, Phe649, Glu802, Leu873,Ser876, Glu879, Arg880, Phe1009, Thr1010, Val1011 and Phe1013 located within the active cavity of the enzyme, insertion of chrysin into the active site occupying the catalytic center of xanthine oxidase to avoid the entrance of xanthine and causing conformational changes in the enzyme. Binding structure, thermodynamic, and kinetic analysis, computational docking. Molecular modeling of enzyme-drug interaction Bos taurus
1.17.3.2 additional information chrysin and its structural analogue apigenin exhibit an additive effect on inhibition of the enzyme, computational docking, overview. Structurally, both chrysin and apigenin possess two-benzene rings, which is very important in the inhibition on the activity of xanthine oxidase, whereas apigenin possesses an extra C-4' hydroxy group which might be the main reason of causing different inhibition compared to chrysin Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.17.3.2 Bos taurus P80457
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.3.2 commercial preparation
-
 Bos taurus
-
1.17.3.2 milk
-
 Bos taurus
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.3.2 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.3.2 7.4
-
 assay at Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.17.3.2 additional information
-
 additional information inhibition kinetic analysis Bos taurus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.17.3.2 0.00126
-
 pH 7.4, 37°C Bos taurus chrysin
1.17.3.2 0.00293
-
 pH 7.4, 37°C Bos taurus allopurinol
1.17.3.2 0.00357
-
 pH 7.4, 37°C Bos taurus apigenin