EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.139 | expressed in Pichia pastoris strain X33 | Scheffersomyces stipitis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.139 | 8.2 | - |
aldotetraouronic acid | at pH 4.0 and 50°C | Scheffersomyces stipitis | |
3.2.1.139 | 9 | - |
aldopentaouronic acid | at pH 4.0 and 50°C | Scheffersomyces stipitis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.139 | Scheffersomyces stipitis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.139 | Ni-Sepharose column chromatography | Scheffersomyces stipitis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.139 | aldopentaouronic acid + H2O | high activity | Scheffersomyces stipitis | 4-O-methylglucuronic acid + ? | - |
? | |
3.2.1.139 | aldotetraouronic acid + H2O | highest activity | Scheffersomyces stipitis | 4-O-methylglucuronic acid + ? | - |
? | |
3.2.1.139 | beechwood xylan + H2O | low activity | Scheffersomyces stipitis | 4-O-methylglucuronic acid + ? | - |
? | |
3.2.1.139 | birchwood xylan + H2O | very low activity | Scheffersomyces stipitis | 4-O-methylglucuronic acid + ? | - |
? | |
3.2.1.139 | oat spelt xylan + H2O | negligible activity | Scheffersomyces stipitis | 4-O-methylglucuronic acid + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.139 | ? | x * 150000, SDS-PAGE | Scheffersomyces stipitis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.139 | Agu115 | - |
Scheffersomyces stipitis |
3.2.1.139 | glycosyl hydrolase family 115 alpha-glucuronidase | - |
Scheffersomyces stipitis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.139 | 50 | - |
- |
Scheffersomyces stipitis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.139 | 19.4 | - |
birchwood xylan | at pH 4.0 and 50°C | Scheffersomyces stipitis | |
3.2.1.139 | 24.2 | - |
beechwood xylan | at pH 4.0 and 50°C | Scheffersomyces stipitis | |
3.2.1.139 | 51.1 | - |
aldopentaouronic acid | at pH 4.0 and 50°C | Scheffersomyces stipitis | |
3.2.1.139 | 69.2 | - |
aldotetraouronic acid | at pH 4.0 and 50°C | Scheffersomyces stipitis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.139 | 4 | - |
- |
Scheffersomyces stipitis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.139 | 3 | 8.5 | - |
Scheffersomyces stipitis |