EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.6 | expression in Pichia pastoris | Coprinopsis cinerea |
3.2.1.39 | expressed in Pichia pastoris GS115 cells | Coprinopsis cinerea |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.6 | Cu2+ | 1 mM, 5% of initial activity | Coprinopsis cinerea | |
3.2.1.6 | EDTA | 1 mM, 84% of initial activity | Coprinopsis cinerea | |
3.2.1.6 | SDS | 1 mM, 72% of initial activity | Coprinopsis cinerea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.6 | additional information | - |
Barley beta-glucan | Km value 20.8 mg/ml, pH 6.0, 50°C | Coprinopsis cinerea | |
3.2.1.6 | additional information | - |
Laminarin | Km value 6.5 mg/ml, pH 6.0, 50°C | Coprinopsis cinerea |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.6 | Ca2+ | 1 mM, 130% of initial activity | Coprinopsis cinerea | |
3.2.1.6 | Co2+ | 1 mM, 143% of initial activity | Coprinopsis cinerea | |
3.2.1.6 | Fe3+ | 1 mM, 116% of initial activity | Coprinopsis cinerea | |
3.2.1.6 | Mg2+ | 1 mM, 113% of initial activity | Coprinopsis cinerea | |
3.2.1.6 | Mn2+ | 1 mM, 215% of initial activity | Coprinopsis cinerea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.6 | Coprinopsis cinerea | A8N2G5 | - |
- |
3.2.1.6 | Coprinopsis cinerea ATCC MYA-4618 | A8N2G5 | - |
- |
3.2.1.39 | Coprinopsis cinerea | A8N2G5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.6 | recombinant protein | Coprinopsis cinerea |
3.2.1.39 | Ni-NTA resin column chromatography, and Superdex 200 gel filtration | Coprinopsis cinerea |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.6 | mycelium | high expression during mycelial stage | Coprinopsis cinerea | - |
3.2.1.39 | fruit body | - |
Coprinopsis cinerea | - |
3.2.1.39 | mycelium | - |
Coprinopsis cinerea | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.6 | 35.5 | - |
substrate laminarin, pH 6.0, 37°C | Coprinopsis cinerea |
3.2.1.6 | 39.8 | - |
substrate periodate-oxidized laminarin, pH 6.0, 37°C | Coprinopsis cinerea |
3.2.1.6 | 72.1 | - |
substrate carboxymethylpachyman, pH 6.0, 37°C | Coprinopsis cinerea |
3.2.1.6 | 118.8 | - |
substrate barley beta-glucan, pH 6.0, 37°C | Coprinopsis cinerea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.6 | barley beta-glucan + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
3.2.1.6 | carboxymethylpachyman + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
3.2.1.6 | laminarin + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
3.2.1.6 | laminaritetraose + H2O | - |
Coprinopsis cinerea | 2 laminaribiose | - |
? | |
3.2.1.6 | additional information | enzyme only acts on substrates containing beta-1,3 glycosidic bonds but not on substrates containing only beta-1,4- or beta-1,6-glycosidic bonds | Coprinopsis cinerea | ? | - |
? | |
3.2.1.6 | periodate-oxidized laminarin + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
3.2.1.39 | barley beta-glucan + H2O | highest activity | Coprinopsis cinerea | ? | - |
? | |
3.2.1.39 | barley beta-glucan + H2O | highest activity | Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? | |
3.2.1.39 | carboxymethyl-pachyman + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
3.2.1.39 | carboxymethyl-pachyman + H2O | - |
Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? | |
3.2.1.39 | laminarin + H2O | lowest activity | Coprinopsis cinerea | ? | - |
? | |
3.2.1.39 | laminarin + H2O | lowest activity | Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? | |
3.2.1.39 | additional information | the enzyme does not act on substrates containing only beta-1,4-glycosidic bondas (like carboxymethyl cellulose) or beta-1,6-glycosidic bonds (like pustulan) | Coprinopsis cinerea | ? | - |
? | |
3.2.1.39 | additional information | the enzyme does not act on substrates containing only beta-1,4-glycosidic bondas (like carboxymethyl cellulose) or beta-1,6-glycosidic bonds (like pustulan) | Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? | |
3.2.1.39 | periodate-oxidized laminarin + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
3.2.1.39 | periodate-oxidized laminarin + H2O | - |
Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.39 | ? | x * 60000, SDS-PAGE | Coprinopsis cinerea |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.6 | CC1G_01665 | - |
Coprinopsis cinerea |
3.2.1.6 | ENG16A | - |
Coprinopsis cinerea |
3.2.1.39 | endo-beta-1,3(4)-glucanase | - |
Coprinopsis cinerea |
3.2.1.39 | ENG16A | - |
Coprinopsis cinerea |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.6 | 60 | - |
- |
Coprinopsis cinerea |
3.2.1.39 | 60 | - |
- |
Coprinopsis cinerea |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.6 | 70 | - |
20% of maximum activity | Coprinopsis cinerea |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.6 | 40 | - |
stable up to | Coprinopsis cinerea |
3.2.1.39 | 20 | 40 | the enzyme can maintain its stability up to 40°C, while when the temperature is at 50°C or above, the activityof the enzyme decreases dramatically | Coprinopsis cinerea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.39 | 6 | - |
- |
Coprinopsis cinerea |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.39 | 5 | 7 | the enzyme retains more than 70% activity between pH 5.0 and 7.0 | Coprinopsis cinerea |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.39 | 4 | 6 | the enzyme retains more than 60% activity between pH 4.0 and 6.0 after 30 min incubation | Coprinopsis cinerea |