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Literature summary extracted from

  • Wang, J.; Kang, L.; Liu, Z.; Yuan, S.
    Gene cloning, heterologous expression and characterization of a Coprinopsis cinerea endo-beta-1,3(4)-glucanase (2017), Fungal Biol., 121, 61-68 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 expression in Pichia pastoris Coprinopsis cinerea
3.2.1.39 expressed in Pichia pastoris GS115 cells Coprinopsis cinerea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.6 Cu2+ 1 mM, 5% of initial activity Coprinopsis cinerea
3.2.1.6 EDTA 1 mM, 84% of initial activity Coprinopsis cinerea
3.2.1.6 SDS 1 mM, 72% of initial activity Coprinopsis cinerea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.6 additional information
-
Barley beta-glucan Km value 20.8 mg/ml, pH 6.0, 50°C Coprinopsis cinerea
3.2.1.6 additional information
-
Laminarin Km value 6.5 mg/ml, pH 6.0, 50°C Coprinopsis cinerea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.6 Ca2+ 1 mM, 130% of initial activity Coprinopsis cinerea
3.2.1.6 Co2+ 1 mM, 143% of initial activity Coprinopsis cinerea
3.2.1.6 Fe3+ 1 mM, 116% of initial activity Coprinopsis cinerea
3.2.1.6 Mg2+ 1 mM, 113% of initial activity Coprinopsis cinerea
3.2.1.6 Mn2+ 1 mM, 215% of initial activity Coprinopsis cinerea

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Coprinopsis cinerea A8N2G5
-
-
3.2.1.6 Coprinopsis cinerea ATCC MYA-4618 A8N2G5
-
-
3.2.1.39 Coprinopsis cinerea A8N2G5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.6 recombinant protein Coprinopsis cinerea
3.2.1.39 Ni-NTA resin column chromatography, and Superdex 200 gel filtration Coprinopsis cinerea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.6 mycelium high expression during mycelial stage Coprinopsis cinerea
-
3.2.1.39 fruit body
-
Coprinopsis cinerea
-
3.2.1.39 mycelium
-
Coprinopsis cinerea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.6 35.5
-
substrate laminarin, pH 6.0, 37°C Coprinopsis cinerea
3.2.1.6 39.8
-
substrate periodate-oxidized laminarin, pH 6.0, 37°C Coprinopsis cinerea
3.2.1.6 72.1
-
substrate carboxymethylpachyman, pH 6.0, 37°C Coprinopsis cinerea
3.2.1.6 118.8
-
substrate barley beta-glucan, pH 6.0, 37°C Coprinopsis cinerea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 barley beta-glucan + H2O
-
Coprinopsis cinerea ?
-
?
3.2.1.6 carboxymethylpachyman + H2O
-
Coprinopsis cinerea ?
-
?
3.2.1.6 laminarin + H2O
-
Coprinopsis cinerea ?
-
?
3.2.1.6 laminaritetraose + H2O
-
Coprinopsis cinerea 2 laminaribiose
-
?
3.2.1.6 additional information enzyme only acts on substrates containing beta-1,3 glycosidic bonds but not on substrates containing only beta-1,4- or beta-1,6-glycosidic bonds Coprinopsis cinerea ?
-
?
3.2.1.6 periodate-oxidized laminarin + H2O
-
Coprinopsis cinerea ?
-
?
3.2.1.39 barley beta-glucan + H2O highest activity Coprinopsis cinerea ?
-
?
3.2.1.39 barley beta-glucan + H2O highest activity Coprinopsis cinerea ATCC MYA-4618 ?
-
?
3.2.1.39 carboxymethyl-pachyman + H2O
-
Coprinopsis cinerea ?
-
?
3.2.1.39 carboxymethyl-pachyman + H2O
-
Coprinopsis cinerea ATCC MYA-4618 ?
-
?
3.2.1.39 laminarin + H2O lowest activity Coprinopsis cinerea ?
-
?
3.2.1.39 laminarin + H2O lowest activity Coprinopsis cinerea ATCC MYA-4618 ?
-
?
3.2.1.39 additional information the enzyme does not act on substrates containing only beta-1,4-glycosidic bondas (like carboxymethyl cellulose) or beta-1,6-glycosidic bonds (like pustulan) Coprinopsis cinerea ?
-
?
3.2.1.39 additional information the enzyme does not act on substrates containing only beta-1,4-glycosidic bondas (like carboxymethyl cellulose) or beta-1,6-glycosidic bonds (like pustulan) Coprinopsis cinerea ATCC MYA-4618 ?
-
?
3.2.1.39 periodate-oxidized laminarin + H2O
-
Coprinopsis cinerea ?
-
?
3.2.1.39 periodate-oxidized laminarin + H2O
-
Coprinopsis cinerea ATCC MYA-4618 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 60000, SDS-PAGE Coprinopsis cinerea

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 CC1G_01665
-
Coprinopsis cinerea
3.2.1.6 ENG16A
-
Coprinopsis cinerea
3.2.1.39 endo-beta-1,3(4)-glucanase
-
Coprinopsis cinerea
3.2.1.39 ENG16A
-
Coprinopsis cinerea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 60
-
-
Coprinopsis cinerea
3.2.1.39 60
-
-
Coprinopsis cinerea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.6 70
-
20% of maximum activity Coprinopsis cinerea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 40
-
stable up to Coprinopsis cinerea
3.2.1.39 20 40 the enzyme can maintain its stability up to 40°C, while when the temperature is at 50°C or above, the activityof the enzyme decreases dramatically Coprinopsis cinerea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 6
-
-
Coprinopsis cinerea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 5 7 the enzyme retains more than 70% activity between pH 5.0 and 7.0 Coprinopsis cinerea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 4 6 the enzyme retains more than 60% activity between pH 4.0 and 6.0 after 30 min incubation Coprinopsis cinerea