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Literature summary extracted from

  • Abrashev, R.; Feller, G.; Kostadinova, N.; Krumova, E.; Alexieva, Z.; Gerginova, M.; Spasova, B.; Miteva-Staleva, J.; Vassilev, S.; Angelova, M.
    Production, purification, and characterization of a novel cold-active superoxide dismutase from the Antarctic strain Aspergillus glaucus 363 (2016), Fungal Biol., 120, 679-689 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 isozyme SODI, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis Aspergillus glaucus
1.15.1.1 isozyme SODII, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis Aspergillus glaucus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ a Cu/ZnSOD Aspergillus glaucus
1.15.1.1 additional information presence of Cu and Zn is confirmed by inductively coupled plasma mass spectrometry, metal content analysis reveals a 1/1 molar ratio (metal/protein) for Cu and Zn, without significant amounts of Fe, Mn or Ni Aspergillus glaucus
1.15.1.1 additional information presence of Cu and Zn is confirmed by inductively coupled plasma mass spectrometry, metal content analysis reveals for SODI molar ratios (metal/protein) for Cu = 0.7 and Zn = 0.4, and for Mn = 0.16 Aspergillus glaucus
1.15.1.1 Zn2+ a Cu/ZnSOD Aspergillus glaucus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+ Aspergillus glaucus
-
O2 + H2O2
-
?
1.15.1.1 2 superoxide + 2 H+ Aspergillus glaucus 363/NBIMCC 8861
-
O2 + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Aspergillus glaucus
-
-
-
1.15.1.1 Aspergillus glaucus A0A1L9VGR0
-
-
1.15.1.1 Aspergillus glaucus 363/NBIMCC 8861
-
-
-
1.15.1.1 Aspergillus glaucus 363/NBIMCC 8861 A0A1L9VGR0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.15.1.1 glycoprotein glycosylation analysis, overview Aspergillus glaucus
1.15.1.1 no glycoprotein glycosylation analysis, overview Aspergillus glaucus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 native isozyme SODI by 23fold gel filtration, hydrophobic interaction chromatography, and anion-exchange chromatography Aspergillus glaucus
1.15.1.1 native isozyme SODII by 153fold gel filtration, hydrophobic interaction chromatography, and anion-exchange chromatography to homogeneity Aspergillus glaucus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 mycelium submerged cultivation Aspergillus glaucus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 623
-
purified native isozyme SODI, pH and temperature not specified in the publication Aspergillus glaucus
1.15.1.1 4000
-
purified native isozyme SODII, pH and temperature not specified in the publication Aspergillus glaucus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+
-
Aspergillus glaucus O2 + H2O2
-
?
1.15.1.1 2 superoxide + 2 H+
-
Aspergillus glaucus 363/NBIMCC 8861 O2 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 ? x * 15835, mass spectrometry and dynamic light scattering Aspergillus glaucus
1.15.1.1 ? x * 38159, mass spectrometry and dynamic light scattering Aspergillus glaucus

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 cold-active superoxide dismutase
-
Aspergillus glaucus
1.15.1.1 Cu/Zn superoxide dismutase
-
Aspergillus glaucus
1.15.1.1 Cu/Zn-SODI
-
Aspergillus glaucus
1.15.1.1 Cu/Zn-SODII
-
Aspergillus glaucus
1.15.1.1 SOD
-
Aspergillus glaucus
1.15.1.1 SODI
-
Aspergillus glaucus
1.15.1.1 SODII
-
Aspergillus glaucus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 additional information
-
SODI displays a Tm value of 54°C and therefore its structure is rather labile to temperature Aspergillus glaucus
1.15.1.1 additional information
-
SODII displays a Tm value of 80°C and therefore its structure is rather stable to temperature Aspergillus glaucus

General Information

EC Number General Information Comment Organism
1.15.1.1 evolution the N-terminal amino acid sequence of Cu/Zn-SODII reveals a high degree of structural homology with Cu/Zn-SOD from other fungi, including Aspergillus species Aspergillus glaucus
1.15.1.1 physiological function the major isoenzyme is Cu/Zn-SODII, the minor Cu/Zn-SODI Aspergillus glaucus