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Literature summary extracted from

  • Liu, T.; Yao, R.; Zhao, Y.; Xu, S.; Huang, C.; Luo, J.; Kong, L.
    Cloning, functional characterization and site-directed mutagenesis of 4-coumarate coenzyme A ligase (4CL) involved in coumarin biosynthesis in Peucedanum praeruptorum Dunn (2017), Front. Plant Sci., 8, 4-4 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expressed in Escherichia coli BL21(DE3) cells Peucedanum praeruptorum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.12 cytosol
-
 Peucedanum praeruptorum 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Peucedanum praeruptorum
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Peucedanum praeruptorum A0A1L7H9I4
-
-
6.2.1.12 Peucedanum praeruptorum A0A1L7H9K0
-
-
6.2.1.12 Peucedanum praeruptorum A0A1L7H9K1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 Ni-NTA column chromatography Peucedanum praeruptorum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 leaf
-
 Peucedanum praeruptorum
-
6.2.1.12 root
-
 Peucedanum praeruptorum
-
6.2.1.12 root isoform 4CL1 is the most highly expressed gene in roots Peucedanum praeruptorum
-
6.2.1.12 seedling
-
 Peucedanum praeruptorum
-
6.2.1.12 stem
-
 Peucedanum praeruptorum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 2-coumarate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + 2-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + isoferulate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + isoferuloyl-CoA
-
 ?
6.2.1.12 additional information isoform 4CL10 has no catalytic activity toward hydroxycinnamic acid compounds Peucedanum praeruptorum ?
-
 ?
6.2.1.12 additional information isoform 4CL7 has no catalytic activity toward hydroxycinnamic acid compounds Peucedanum praeruptorum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumarate: coenzyme A ligase
-
 Peucedanum praeruptorum
6.2.1.12 4CL1 isoform Peucedanum praeruptorum
6.2.1.12 4CL10 isoform Peucedanum praeruptorum
6.2.1.12 4CL7 isoform Peucedanum praeruptorum

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Peucedanum praeruptorum

Expression

EC Number Organism Comment Expression
6.2.1.12 Peucedanum praeruptorum upon PEG, NaCl, CuSO4, heat and cold treatments, the expression level of isoform 4CL1 significantly decreases down
6.2.1.12 Peucedanum praeruptorum the expression level of isoform 4CL10 is clearly enhanced under PEG treatment up
6.2.1.12 Peucedanum praeruptorum transcript abundance of isoform 4CL7 is up-regulated under heat treatment up