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Literature summary extracted from
- Characterization of a stearoyl-acyl carrier protein desaturase gene family from chocolate tree, Theobroma cacao L (2015), Front. Plant Sci., 6, 239 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.2 | gene SAD1, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter | Theobroma cacao |
| 1.14.19.2 | gene SAD2, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter | Theobroma cacao |
| 1.14.19.2 | gene SAD3, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter | Theobroma cacao |
| 1.14.19.2 | gene SAD4, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter | Theobroma cacao |
| 1.14.19.2 | gene SAD5, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter | Theobroma cacao |
| 1.14.19.2 | gene SAD6, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter | Theobroma cacao |
| 1.14.19.2 | gene SAD7, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, SAD7 cannot be recombinantly expressed in Arabidopsis thaliana | Theobroma cacao |
| 1.14.19.2 | gene SAD8, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis | Theobroma cacao |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.19.2 | chloroplast stroma | - |
Theobroma cacao | 9570 | - |
| 1.14.19.2 | mitochondrion | a mitochondrial targeting peptide is predicted in TcSAD3 | Theobroma cacao | 5739 | - |
| 1.14.19.2 | additional information | a signal peptide related with the secretory pathway is detected in TcSAD4 | Theobroma cacao | - |
- |
| 1.14.19.2 | soluble | - |
Theobroma cacao | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.2 | Fe2+ | ferredoxin-[iron-sulfur] cluster | Theobroma cacao |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Theobroma cacao | - |
oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.2 | Theobroma cacao | A0A0F6SYT9 | - |
- |
| 1.14.19.2 | Theobroma cacao | A0A0F6SYU0 | - |
- |
| 1.14.19.2 | Theobroma cacao | A0A0F6X2R5 | - |
- |
| 1.14.19.2 | Theobroma cacao | A0A0F6X2V1 | - |
- |
| 1.14.19.2 | Theobroma cacao | A0A0F6ZN15 | - |
- |
| 1.14.19.2 | Theobroma cacao | A0A0F6ZN93 | - |
- |
| 1.14.19.2 | Theobroma cacao | A0A0F6ZNE1 | - |
- |
| 1.14.19.2 | Theobroma cacao | A0A0F6ZQT3 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.19.2 | embryo | - |
Theobroma cacao | - |
| 1.14.19.2 | flower | - |
Theobroma cacao | - |
| 1.14.19.2 | leaf | - |
Theobroma cacao | - |
| 1.14.19.2 | additional information | tissue-specific expression pattern of SAD isoforms in Theobroma cacao, expression of TcSAD5 is primarily detected in roots at low level | Theobroma cacao | - |
| 1.14.19.2 | additional information | tissue-specific expression pattern of SAD isoforms in Theobroma cacao, genes TcSAD3 and TcSAD4 are predominantly expressed in unopened and open flowers, where the expression levels of TcSAD3 and TcSAD4 are significantly higher than any other SAD isoforms, the transcripts of both are barely detectable in all the other tissues, indicating that they are flower-specific genes | Theobroma cacao | - |
| 1.14.19.2 | additional information | tissue-specific expression pattern of SAD isoforms in Theobroma cacao, TcSAD7 constitutivel expresses in all examined tissues with higher expression in roots and zygotic seeds at all developmental stages | Theobroma cacao | - |
| 1.14.19.2 | additional information | tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD1 and TcSAD2 are detected in all the examined tissues, in which the expression levels of TcSAD1 are higher than those of TcSAD2 | Theobroma cacao | - |
| 1.14.19.2 | additional information | tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD6 and TcSAD8 are barely detected in any of the tissues analyzed, suggesting that they might be pseudogenes or expressed in a tissue, stage of development, or induction condition not tested | Theobroma cacao | - |
| 1.14.19.2 | root | - |
Theobroma cacao | - |
| 1.14.19.2 | seed | zygotic, TcSAD relative expression and fatty acid composition in maturing cacao seeds, overview | Theobroma cacao | - |
| 1.14.19.2 | seed | zygotic, TcSAD relative expression and fatty acid composition in maturing cacao seeds, overview, highest expression level of TcSAD7 | Theobroma cacao | - |
| 1.14.19.2 | seed | zygotic, TcSAD relative expression and fatty acid composition in maturing cacao seeds, overview. High expression level of TcSAD1 | Theobroma cacao | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Theobroma cacao | oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.19.2 | ? | x * 33910, sequence calculation | Theobroma cacao |
| 1.14.19.2 | ? | x * 3794 0, sequence calculation | Theobroma cacao |
| 1.14.19.2 | ? | x * 41860, sequence calculation | Theobroma cacao |
| 1.14.19.2 | ? | x * 44080, sequence calculation | Theobroma cacao |
| 1.14.19.2 | ? | x * 44760, sequence calculation | Theobroma cacao |
| 1.14.19.2 | ? | x * 44830, sequence calculation | Theobroma cacao |
| 1.14.19.2 | ? | x * 45310, sequence calculation | Theobroma cacao |
| 1.14.19.2 | ? | x * 45390, sequence calculation | Theobroma cacao |
| 1.14.19.2 | More | cacao SADs consist of 11 highly conserved alpha-helices. SAD isozymes share conserved overall protein structures in Theobroma cacao | Theobroma cacao |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.2 | SAD | - |
Theobroma cacao |
| 1.14.19.2 | SAD1 | - |
Theobroma cacao |
| 1.14.19.2 | SAD2 | - |
Theobroma cacao |
| 1.14.19.2 | SAD3 | - |
Theobroma cacao |
| 1.14.19.2 | SAD4 | - |
Theobroma cacao |
| 1.14.19.2 | SAD6 | - |
Theobroma cacao |
| 1.14.19.2 | SAD7 | - |
Theobroma cacao |
| 1.14.19.2 | SAD8 | - |
Theobroma cacao |
| 1.14.19.2 | stearoyl-ACP desaturase | - |
Theobroma cacao |
| 1.14.19.2 | stearoyl-acyl carrier protein desaturase | - |
Theobroma cacao |
| 1.14.19.2 | Tc01g009910 | locus name | Theobroma cacao |
| 1.14.19.2 | Tc04g005590 | locus name | Theobroma cacao |
| 1.14.19.2 | Tc04g017510 | locus name | Theobroma cacao |
| 1.14.19.2 | Tc04g017520 | locus name | Theobroma cacao |
| 1.14.19.2 | Tc04g017540 | locus name | Theobroma cacao |
| 1.14.19.2 | Tc05g012840 | locus name | Theobroma cacao |
| 1.14.19.2 | Tc08g012550 | locus name | Theobroma cacao |
| 1.14.19.2 | Tc09g024040 | locus name | Theobroma cacao |
| 1.14.19.2 | TcSAD1 | - |
Theobroma cacao |
| 1.14.19.2 | TcSAD2 | - |
Theobroma cacao |
| 1.14.19.2 | TcSAD3 | - |
Theobroma cacao |
| 1.14.19.2 | TcSAD4 | - |
Theobroma cacao |
| 1.14.19.2 | TcSAD5 | - |
Theobroma cacao |
| 1.14.19.2 | TcSAD6 | - |
Theobroma cacao |
| 1.14.19.2 | TcSAD7 | - |
Theobroma cacao |
| 1.14.19.2 | TcSAD8 | - |
Theobroma cacao |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.2 | Ferredoxin | - |
Theobroma cacao |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.19.2 | Theobroma cacao | sequence calculation | - |
5.33 |
| 1.14.19.2 | Theobroma cacao | sequence calculation | - |
5.49 |
| 1.14.19.2 | Theobroma cacao | sequence calculation | - |
5.87 |
| 1.14.19.2 | Theobroma cacao | sequence calculation | - |
5.88 |
| 1.14.19.2 | Theobroma cacao | sequence calculation | - |
6.05 |
| 1.14.19.2 | Theobroma cacao | sequence calculation | - |
6.24 |
| 1.14.19.2 | Theobroma cacao | sequence calculation | - |
6.39 |
| 1.14.19.2 | Theobroma cacao | sequence calculation | - |
6.51 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.19.2 | physiological function | activity of TcSAD1 is possibly involved in the synthesis and accumulation of oleate in cacao embryos | Theobroma cacao |
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