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Literature summary extracted from

  • Ma, J.C.; Wu, Y.Q.; Cao, D.; Zhang, W.B.; Wang, H.H.
    Only acyl carrier protein 1 (AcpP1) functions in Pseudomonas aeruginosa fatty acid synthesis (2017), Front. Microbiol., 8, 2186 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.20 gene aasS, recombinant expression of His-tagged enzyme in Escherichia coli Vibrio harveyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.20 Mg2+ required Pseudomonas aeruginosa
6.2.1.20 Mg2+ required Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.20 ATP + a long-chain fatty acid + an [acyl-carrier protein] Pseudomonas aeruginosa
-
 AMP + diphosphate + a long-chain acyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + a long-chain fatty acid + an [acyl-carrier protein] Vibrio harveyi
-
 AMP + diphosphate + a long-chain acyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Pseudomonas aeruginosa Q9I4H2
-
-
6.2.1.20 Pseudomonas aeruginosa
-
-
-
6.2.1.20 Vibrio harveyi Q00IB3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.20 recombinant His-tagged enzyme from Escherichia coli Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA + apo-[peptidyl-carrier protein 1]
-
 Pseudomonas aeruginosa adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
 ?
2.7.8.7 CoA + apo-[peptidyl-carrier protein 2]
-
 Pseudomonas aeruginosa adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
 ?
2.7.8.7 CoA + apo-[peptidyl-carrier protein 3]
-
 Pseudomonas aeruginosa adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
 ?
6.2.1.20 ATP + a long-chain fatty acid + an [acyl-carrier protein]
-
 Pseudomonas aeruginosa AMP + diphosphate + a long-chain acyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + a long-chain fatty acid + an [acyl-carrier protein]
-
 Vibrio harveyi AMP + diphosphate + a long-chain acyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + octanoate + [AcpP1] recombinant holo-AcpP1 from Pseudomonas aeruginosa Vibrio harveyi AMP + diphosphate + octanoyl-[AcpP1]
-
 ?
6.2.1.20 ATP + octanoate + [AcpP2] recombinant holo-AcpP2 from Pseudomonas aeruginosa Vibrio harveyi AMP + diphosphate + octanoyl-[AcpP2]
-
 ?
6.2.1.20 ATP + octanoate + [AcpP3] recombinant holo-AcpP3 from Pseudomonas aeruginosa Vibrio harveyi AMP + diphosphate + octanoyl-[AcpP3]
-
 ?
6.2.1.20 additional information the cell-free extract of Pseudomonas aeruginosa can acylate only the holo-form of the acyl carrier protein AcpP1 for the synthesis of fatty acids, while it is inactive with acyl carrier proteins AcpP2 and AcpP3 Pseudomonas aeruginosa ?
-
 ?
6.2.1.20 additional information the enzyme from Vibrio harveyi can acylate holo-forms of the acyl carrier proteins, AcpP1, AcpP2, and AcpP3, from Pseudomonas aeruginosa Vibrio harveyi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 PA1165
-
 Pseudomonas aeruginosa
2.7.8.7 PcpS
-
 Pseudomonas aeruginosa
6.2.1.20 AasS
-
 Pseudomonas aeruginosa
6.2.1.20 AasS
-
 Vibrio harveyi
6.2.1.20 Acyl-ACP synthetase
-
 Pseudomonas aeruginosa
6.2.1.20 Acyl-ACP synthetase
-
 Vibrio harveyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.20 37
-
 assay at Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.20 7.8
-
 assay at Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.20 ATP
-
 Pseudomonas aeruginosa
6.2.1.20 ATP
-
 Vibrio harveyi

General Information

EC Number General Information Comment Organism
2.7.8.7 physiological function Pseudomonas aeruginosa acyl carrier proteins, AcpP1, AcpP2, and AcpP3, are successfully phosphopantetheinylated by PcpS, while only AcpP1 can be used as a substrate for the synthesis of fatty acids in cell free extracts in vitro Pseudomonas aeruginosa
6.2.1.20 malfunction Pseudomonas aeruginosa acpP1 deletion is lethal, while disruption of acpP2 or acpP3 in the Pseudomonas aeruginosa genome allowa mutant strains to grow like the wild-type strain. AcpP1 functions in fatty acid biosynthesis with the enzyme acyl-ACP synthetase, acpP2 and acpP3 do not play roles in the fatty acid synthetic pathway. Replacement of acpP1 with Escherichia coli acpP reduces the ability of Pseudomonas aeruginosa to produce some exo-products and abolished swarming motility Pseudomonas aeruginosa
6.2.1.20 physiological function only the acpP1 gene can restore growth in the Escherichia coli acpP mutant strain CY1877, while genes acpP2 and acpP3 are ineffective, neither AcpP2 nor AcpP3 can act as a substrate for synthesis of N-acylhomoserine lactones molecules in vivo. Gene acpP1 is essential for Pseudomonas aeruginosa growth Pseudomonas aeruginosa