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Literature summary extracted from

  • Zorin, N.; Medina, M.; Pusheva, M.; Gogotov, I.; Cammack, R.
    Hydrogenase from the thermophilic bacterium Thermococcus stetteri Isolation and characterisation of EPR-detectable redox centres (1996), FEMS Microbiol. Lett., 142, 71-76 .
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.2 Iron [NiFe]-hydrogenase, the ratio of iron to nickel is 13 gatom Fe/1 gatom Ni. The absorption spectrum is typical of iron-sulfur proteins having [4Fe-4S] clusters Thermococcus stetteri
1.12.1.2 Nickel [NiFe]-hydrogenase, the ratio of iron to nickel is 13 gatom Fe/1 gatom Ni Thermococcus stetteri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.1.2 106000
-
 non-denaturing PAGE Thermococcus stetteri

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Thermococcus stetteri
-
-
-
1.12.1.2 Thermococcus stetteri K-15
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.2
-
 Thermococcus stetteri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 430
-
 pH 6.0, 40°C, measured by hydrogen uptake with methyl viologen as electron acceptor Thermococcus stetteri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + oxidized methyl viologen
-
 Thermococcus stetteri H+ + reduced methyl viologen
-
 ?
1.12.1.2 H2 + oxidized methyl viologen
-
 Thermococcus stetteri K-15 H+ + reduced methyl viologen
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.1.2 40
-
 assay at Thermococcus stetteri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.1.2 8
-
 assay at Thermococcus stetteri

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.2 FMN enzyme contains FMN Thermococcus stetteri