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Literature summary extracted from

  • Vazquez, C.; Mejia-Tlachi, M.; Gonzalez-Chavez, Z.; Silva, A.; Rodriguez-Zavala, J.S.; Moreno-Sanchez, R.; Saavedra, E.
    Buthionine sulfoximine is a multitarget inhibitor of trypanothione synthesis in Trypanosoma cruzi (2017), FEBS Lett., 591, 3881-3894 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.9 gene tryS, recombinant expression in Escherichia coli strain BL21(DE3) Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.9 DL-(S,R)-buthionine sulfoximine DL-(S,R)-BSO, a multitarget inhibitor of trypanothione synthesis, in vivo effects on nonthiol-supplied epimastigote cells, on Cys-supplied epimastigote cells, and on GSH-supplied epimastigote cells, overview. Inhibition of gamma-glutamylcysteine synthetase and trypanothione synthetase. Wild-type Trypanosoma brucei cells are not restored by supplementing with 0.08 mM GSH. The the L-(S,R)-BSO stereoisomer is more potent that the the D-(S,R)-BSO. Inhibitor docking analysis, binding structure Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.9 Mg2+ required Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.9 glutathione + glutathionylspermidine + ATP Trypanosoma cruzi
-
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + glutathionylspermidine + ATP Trypanosoma cruzi DTU I
-
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + spermidine + ATP Trypanosoma cruzi
-
glutathionylspermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + spermidine + ATP Trypanosoma cruzi DTU I
-
glutathionylspermidine + ADP + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.9 Trypanosoma cruzi
-
-
-
6.3.1.9 Trypanosoma cruzi DTU I
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.9 recombinant enzyme from Escherichia coli strain BL21(DE3) Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.9 epimastigote
-
Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
Trypanosoma cruzi N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
Trypanosoma cruzi DTU I N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + spermidine + ATP
-
Trypanosoma cruzi glutathionylspermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + spermidine + ATP
-
Trypanosoma cruzi DTU I glutathionylspermidine + ADP + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.9 TcTryS
-
Trypanosoma cruzi
6.3.1.9 TryS
-
Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.9 37
-
assay at Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.9 7.4
-
assay at Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.9 ATP
-
Trypanosoma cruzi

General Information

EC Number General Information Comment Organism
6.3.1.9 additional information docking study and structure homology modeling using Leishmania major TryS, PDB ID 2VOB, structure as template, overview Trypanosoma cruzi