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Literature summary extracted from

  • Matsui, I.; Sakai, Y.; Matsui, E.; Kikuchi, H.; Kawarabayasi, Y.; Honda, K.
    Novel substrate specificity of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii (2000), FEBS Lett., 467, 195-200 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B40 expression in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B40 0.03
-
 n-dodecyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 0.05
-
 n-undecyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 0.08
-
 n-decyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 0.08
-
 n-nonyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 0.1
-
 4-nitrophenyl beta-D-xylopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 0.14
-
 4-nitrophenyl beta-D-mannopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 0.2
-
 n-octyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 0.35
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 0.54
-
 n-hexyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 1.3
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 1.96
-
 Salicin pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 2.02
-
 n-amyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 40.74
-
 methyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 138.23
-
 beta-D-Glc-(1->3)-beta-D-Glc pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 1698.18
-
 beta-D-Glc-(1->4)-beta-D-Glc pH 6.0, 90°C Pyrococcus horikoshii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.B40 membrane
-
 Pyrococcus horikoshii 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B40 Pyrococcus horikoshii O58104
-
-
3.2.1.B40 Pyrococcus horikoshii OT-3 O58104
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B40
-
 Pyrococcus horikoshii

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.B40 denaturation with 8 M urea and the renaturation by direct dilution with buffer Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B40 4-nitrophenyl beta-D-galactopyranoside + H2O kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside Pyrococcus horikoshii 4-nitrophenol + D-galactose
-
 ?
3.2.1.B40 4-nitrophenyl beta-D-galactopyranoside + H2O kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside Pyrococcus horikoshii OT-3 4-nitrophenol + D-galactose
-
 ?
3.2.1.B40 4-nitrophenyl beta-D-glucopyranoside + H2O kcat/Km-values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside Pyrococcus horikoshii 4-nitrophenol + D-glucose
-
 ?
3.2.1.B40 4-nitrophenyl beta-D-glucopyranoside + H2O kcat/Km-values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside Pyrococcus horikoshii OT-3 4-nitrophenol + D-glucose
-
 ?
3.2.1.B40 4-nitrophenyl beta-D-mannopyranoside + H2O kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside Pyrococcus horikoshii 4-nitrophenol + D-mannose
-
 ?
3.2.1.B40 4-nitrophenyl beta-D-xylopyranoside + H2O kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside Pyrococcus horikoshii 4-nitrophenol + D-xylose
-
 ?
3.2.1.B40 4-nitrophenyl beta-D-xylopyranoside + H2O kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside Pyrococcus horikoshii OT-3 4-nitrophenol + D-xylose
-
 ?
3.2.1.B40 beta-D-Glc-(1->3)-beta-D-Glc + H2O i.e. laminaribiose, beta-linked glucose dimers are poorly hydrolyzed. The order of preference of His-tagged enzyme is beta-(1->3), beta-(1->4), beta-(1->6) Pyrococcus horikoshii beta-D-glucose
-
 ?
3.2.1.B40 beta-D-Glc-(1->4)-beta-D-Glc + H2O i.e. cellobiose, beta-linked glucose dimers are poorly hydrolyzed. The order of preference of His-tagged enzyme is beta-(1->3), beta-(1->4), beta-(1->6) Pyrococcus horikoshii beta-D-glucose
-
 ?
3.2.1.B40 beta-D-Glc-(1->6)-beta-D-Glc + H2O beta-linked glucose dimers are poorly hydrolyzed. The order of preference of His-tagged enzyme is beta-(1->3), beta-(1->4), beta-(1->6) Pyrococcus horikoshii beta-D-glucose
-
 ?
3.2.1.B40 methyl beta-D-glucopyranoside + H2O
-
 Pyrococcus horikoshii methanol + beta-D-glucose
-
 ?
3.2.1.B40 methyl beta-D-glucopyranoside + H2O
-
 Pyrococcus horikoshii OT-3 methanol + beta-D-glucose
-
 ?
3.2.1.B40 additional information the His-tagged enzyme has approximately 50% of the activity of the enzyme without a tag due to interference by the His-tag located at the N-terminus Pyrococcus horikoshii ?
-
 ?
3.2.1.B40 additional information the His-tagged enzyme has approximately 50% of the activity of the enzyme without a tag due to interference by the His-tag located at the N-terminus Pyrococcus horikoshii OT-3 ?
-
 ?
3.2.1.B40 n-amyl beta-D-glucopyranoside + H2O
-
 Pyrococcus horikoshii 1-pentanol + beta-D-glucose
-
 ?
3.2.1.B40 n-decyl beta-D-glucopyranoside + H2O the best substrates for the His-tagged enzyme are beta-D-glucosides with long alkyl chains Pyrococcus horikoshii 1-decanol + beta-D-glucose
-
 ?
3.2.1.B40 n-dodecyl beta-D-glucopyranoside + H2O the best substrates for the His-tagged enzyme are beta-D-glucosides with long alkyl chains Pyrococcus horikoshii 1-dodecanol + beta-D-glucose
-
 ?
3.2.1.B40 n-hexyl beta-D-glucopyranoside + H2O
-
 Pyrococcus horikoshii 1-hexanol + beta-D-glucose
-
 ?
3.2.1.B40 n-nonyl beta-D-glucopyranoside + H2O
-
 Pyrococcus horikoshii 1-nonanol + beta-D-glucose
-
 ?
3.2.1.B40 n-octyl beta-D-glucopyranoside + H2O
-
 Pyrococcus horikoshii 1-octanol + beta-D-glucose
-
 ?
3.2.1.B40 n-undecyl beta-D-glucopyranoside + H2O the best substrates for the His-tagged enzyme are beta-D-glucosides with long alkyl chains Pyrococcus horikoshii 1-undecanol + beta-D-glucose
-
 ?
3.2.1.B40 salicin + H2O
-
 Pyrococcus horikoshii beta-D-glucose + 2-(hydroxymethyl)phenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B40 BGPh
-
 Pyrococcus horikoshii
3.2.1.B40 PH0366
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B40 100
-
-
 Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B40 90
-
 pH 6.0, half-life: 15 h Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B40 2
-
 4-nitrophenyl beta-D-mannopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 3 6 n-dodecyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 3
-
 4-nitrophenyl beta-D-xylopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 31
-
 n-amyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 33
-
 n-hexyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 34
-
 n-octyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 35
-
 methyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 37
-
 n-decyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 39
-
 n-nonyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 43
-
 n-undecyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 44
-
 Salicin pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 79
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 123
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 184
-
 beta-D-Glc-(1->3)-beta-D-Glc pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 194
-
 beta-D-Glc-(1->4)-beta-D-Glc pH 6.0, 90°C Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B40 6
-
-
 Pyrococcus horikoshii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B40 0.11
-
 beta-D-Glc-(1->4)-beta-D-Glc pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 0.85
-
 methyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 1.33
-
 beta-D-Glc-(1->3)-beta-D-Glc pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 14.6
-
 4-nitrophenyl beta-D-mannopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 15.11
-
 n-amyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 22.2
-
 Salicin pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 31.83
-
 4-nitrophenyl beta-D-xylopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 60.28
-
 n-hexyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 94.34
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 170.7
-
 n-octyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 225.67
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 469.62
-
 n-decyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 471.57
-
 n-nonyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 944.37
-
 n-undecyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii
3.2.1.B40 1152.9
-
 n-dodecyl beta-D-glucopyranoside pH 6.0, 90°C Pyrococcus horikoshii