Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kunze, C.; Diekert, G.; Schubert, T.
    Subtle changes in the active site architecture untangled overlapping substrate ranges and mechanistic differences of two reductive dehalogenases (2017), FEBS J., 284, 3520-3535 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.21.99.5 R357E inactive Desulfitobacterium hafniense
1.21.99.5 R357K the mutant shows about 1% of wild type activity Desulfitobacterium hafniense
1.21.99.5 R357N the mutant shows about 10% of wild type activity Desulfitobacterium hafniense
1.21.99.5 R357Q the mutant shows about 70% of wild type activity Desulfitobacterium hafniense
1.21.99.5 Y298F inactive Desulfitobacterium hafniense
1.21.99.5 Y298H the mutant shows about 30% of wild type activity Desulfitobacterium hafniense
1.21.99.5 Y298S the mutant shows about 3% of wild type activity Desulfitobacterium hafniense
1.21.99.5 Y298T the mutant shows about 10% of wild type activity Desulfitobacterium hafniense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.99.5 trichloroethene + chloride + acceptor Desulfitobacterium hafniense
-
 tetrachloroethene + reduced acceptor
-
 ?
1.21.99.5 trichloroethene + chloride + acceptor Desulfitobacterium hafniense Y51
-
 tetrachloroethene + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.99.5 Desulfitobacterium hafniense
-
-
-
1.21.99.5 Desulfitobacterium hafniense Y51
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.99.5 additional information the enzyme shows no dihaloelimination activity towards 1,1,2-trichloroethane or 1,1,1-trichloroethane Desulfitobacterium hafniense ?
-
 ?
1.21.99.5 additional information the enzyme shows no dihaloelimination activity towards 1,1,2-trichloroethane or 1,1,1-trichloroethane Desulfitobacterium hafniense Y51 ?
-
 ?
1.21.99.5 trichloroethene + chloride + acceptor
-
 Desulfitobacterium hafniense tetrachloroethene + reduced acceptor
-
 ?
1.21.99.5 trichloroethene + chloride + acceptor
-
 Desulfitobacterium hafniense Y51 tetrachloroethene + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.99.5 ? x * 70000, SDS-PAGE Desulfitobacterium hafniense

Synonyms

EC Number Synonyms Comment Organism
1.21.99.5 PCE reductive dehalogenase
-
 Desulfitobacterium hafniense
1.21.99.5 PceA
-
 Desulfitobacterium hafniense
1.21.99.5 tetrachloroethene reductive dehalogenase
-
 Desulfitobacterium hafniense