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Literature summary extracted from

  • Linares-Pasten, J.A.; Falck, P.; Albasri, K.; Kjellstroem, S.; Adlercreutz, P.; Logan, D.T.; Karlsson, E.N.
    Three-dimensional structures and functional studies of two GH43 arabinofuranosidases from Weissella sp. strain 142 and Lactobacillus brevis (2017), FEBS J., 284, 2019-2036 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expressed in Escherichia coli BL21(DE3) cells Levilactobacillus brevis
3.2.1.55 expressed in Escherichia coli BL21(DE3) cells Weissella sp. 142

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 12
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Levilactobacillus brevis
3.2.1.55 13
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Levilactobacillus brevis
3.2.1.55 18
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Weissella sp. 142
3.2.1.55 33
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Weissella sp. 142

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 88000
-
 gel filtration Weissella sp. 142
3.2.1.55 181000
-
 gel filtration Levilactobacillus brevis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Levilactobacillus brevis
-
-
-
3.2.1.55 Levilactobacillus brevis DSM 1269
-
-
-
3.2.1.55 Weissella sp. 142 A0A1L7HA00
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 1,5-alpha-L-arabinobiose + H2O highest activity Levilactobacillus brevis ?
-
 ?
3.2.1.55 1,5-alpha-L-arabinobiose + H2O highest activity Weissella sp. 142 ?
-
 ?
3.2.1.55 1,5-alpha-L-arabinobiose + H2O highest activity Levilactobacillus brevis DSM 1269 ?
-
 ?
3.2.1.55 1,5-alpha-L-arabinotriose + H2O highest activity Levilactobacillus brevis ?
-
 ?
3.2.1.55 1,5-alpha-L-arabinotriose + H2O high activity Weissella sp. 142 ?
-
 ?
3.2.1.55 1,5-alpha-L-arabinotriose + H2O highest activity Levilactobacillus brevis DSM 1269 ?
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Levilactobacillus brevis 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Weissella sp. 142 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Levilactobacillus brevis DSM 1269 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
3.2.1.55 additional information no activity with 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 1,3-L-arabino-beta-D-xylobiose, sugar beet arabinan, and rye arabinoxylan Levilactobacillus brevis ?
-
 ?
3.2.1.55 additional information no activity with 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 1,3-L-arabino-beta-D-xylobiose, sugar beet arabinan, and rye arabinoxylan Weissella sp. 142 ?
-
 ?
3.2.1.55 additional information no activity with 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 1,3-L-arabino-beta-D-xylobiose, sugar beet arabinan, and rye arabinoxylan Levilactobacillus brevis DSM 1269 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 homodimer 2 * 40000, SDS-PAGE Weissella sp. 142
3.2.1.55 homodimer 2 * 39768, calculated from amino acid sequence Weissella sp. 142
3.2.1.55 homotetramer 4 * 40000, SDS-PAGE Levilactobacillus brevis
3.2.1.55 homotetramer 4 * 39784, calculated from amino acid sequence Levilactobacillus brevis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-L-arabinofuranosidase
-
 Levilactobacillus brevis
3.2.1.55 alpha-L-arabinofuranosidase
-
 Weissella sp. 142
3.2.1.55 AraF43
-
 Levilactobacillus brevis
3.2.1.55 AraF43
-
 Weissella sp. 142

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 45
-
-
 Levilactobacillus brevis
3.2.1.55 45
-
-
 Weissella sp. 142

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 45
-
 the enzyme keeps about 40% of its initial activity after 20 min at 45°C Weissella sp. 142
3.2.1.55 45
-
 the enzyme loses all activity after 10 min incubation at 45°C and retains about 40% activity after 1 h at 37°C Levilactobacillus brevis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 57
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Levilactobacillus brevis
3.2.1.55 71
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Levilactobacillus brevis
3.2.1.55 125
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Weissella sp. 142
3.2.1.55 221
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Weissella sp. 142

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5.8
-
-
 Levilactobacillus brevis
3.2.1.55 5.8
-
-
 Weissella sp. 142

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.55 4.8
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Levilactobacillus brevis
3.2.1.55 5.5
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Levilactobacillus brevis
3.2.1.55 6.7
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Weissella sp. 142
3.2.1.55 6.9
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Weissella sp. 142