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Literature summary extracted from

  • Lienhart, W.D.; Strandback, E.; Gudipati, V.; Koch, K.; Binter, A.; Uhl, M.K.; Rantasa, D.M.; Bourgeois, B.; Madl, T.; Zangger, K.; Gruber, K.; Macheroux, P.
    Catalytic competence, structure and stability of the cancer-associated R139W variant of the human NAD(P)H quinone oxidoreductase 1 (NQO1) (2017), FEBS J., 284, 1233-1245 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.2 expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.6.5.2 mutant R139W, to 2.09 A resolution Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.6.5.2 R139W single nucleotide polymorphism, variant protein adopts the same structure both in the crystal as in solution, and kinetic parameters are similar to those reported for the wild-type protein. Thermostability of the variant is only slightly affected Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.0106
-
 menadione mutant R139W, pH 7.0,25°C Homo sapiens
1.6.5.2 0.0116
-
 menadione wild-type, pH 7.0,25°C Homo sapiens
1.6.5.2 3.22
-
 NADH wild-type, pH 7.0,25°C Homo sapiens
1.6.5.2 3.31
-
 NADH mutant R139W, pH 7.0,25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Homo sapiens P15559
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 menadione + NADH + H+
-
 Homo sapiens reduced menadione + NAD+
-
 ?
1.6.5.2 menadione + NADPH + H+
-
 Homo sapiens reduced menadione + NADP+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.5.2 52
-
 melting point, mutant R139W Homo sapiens
1.6.5.2 54
-
 melting point, wild-type Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.2 1580
-
 NADH mutant R139W, pH 7.0,25°C Homo sapiens
1.6.5.2 1590
-
 NADH wild-type, pH 7.0,25°C Homo sapiens
1.6.5.2 1730
-
 menadione mutant R139W, pH 7.0,25°C Homo sapiens
1.6.5.2 1830
-
 menadione wild-type, pH 7.0,25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FAD absorption spectra of wild-type and mutant R139W with maxima at 375 and 450 nm Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6.5.2 478
-
 NADH mutant R139W, pH 7.0,25°C Homo sapiens
1.6.5.2 494
-
 NADH wild-type, pH 7.0,25°C Homo sapiens
1.6.5.2 158000
-
 menadione wild-type, pH 7.0,25°C Homo sapiens
1.6.5.2 162000
-
 menadione mutant R139W, pH 7.0,25°C Homo sapiens