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Literature summary extracted from

  • Matsui, E.; Urushibata, Y.; Abe, J.; Matsui, I.
    Serial intermediates with a 1 nt 3'-flap and 5' variable-length flaps are formed by cooperative functioning of Pyrococcus horikoshii FEN-1 with either B or D DNA polymerases (2014), Extremophiles, 18, 415-427 .
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Pyrococcus horikoshii O57863 and O57861 O57863: DNA polymerase II small subunit, gene name polB, ordered locus name: PH0123, O57861: DNA polymerase II large subunit, gene name polC, ordered locus name: PH0121
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2.7.7.7 Pyrococcus horikoshii OT-3 O57863 and O57861 O57863: DNA polymerase II small subunit, gene name polB, ordered locus name: PH0123, O57861: DNA polymerase II large subunit, gene name polC, ordered locus name: PH0121
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3.1.99.B1 Pyrococcus horikoshii O50123
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3.1.99.B1 Pyrococcus horikoshii OT-3 O50123
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 additional information FEN-1 has both 5'-flap endonuclease and 5'–3' exonuclease activities, FEN-1 activity is elevated by the presence of a 1 nucleotide expansion at the 3' end in the upstream primer of substrates called: structures with a 1 nt 3'-flap, which appear to be the most preferable substrates for FEN-1. Serial intermediates with a 1 nt 3'-flap and 5' variable-length flaps are formed by cooperative functioning of Pyrococcus horikoshii FEN-1 with either B or D DNA polymerases Pyrococcus horikoshii ?
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?
2.7.7.7 additional information FEN-1 has both 5'-flap endonuclease and 5'–3' exonuclease activities, FEN-1 activity is elevated by the presence of a 1 nucleotide expansion at the 3' end in the upstream primer of substrates called: structures with a 1 nt 3'-flap, which appear to be the most preferable substrates for FEN-1. Serial intermediates with a 1 nt 3'-flap and 5' variable-length flaps are formed by cooperative functioning of Pyrococcus horikoshii FEN-1 with either B or D DNA polymerases Pyrococcus horikoshii OT-3 ?
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?
3.1.99.B1 additional information FEN-1 has both 5'-flap endonuclease and 5'-3' exonuclease activities, FEN-1 activity is elevated by the presence of a 1 nucleotide expansion at the 3' end in the upstream primer of substrates called: structures with a 1 nt 3'-flap, which appear to be the most preferable substrates for FEN-1. Serial intermediates with a 1 nt 3'-flap and 5' variable-length flaps are formed by cooperative functioning of Pyrococcus horikoshii FEN-1 with either B or D DNA polymerases Pyrococcus horikoshii ?
-
?
3.1.99.B1 additional information FEN-1 has both 5'-flap endonuclease and 5'-3' exonuclease activities, FEN-1 activity is elevated by the presence of a 1 nucleotide expansion at the 3' end in the upstream primer of substrates called: structures with a 1 nt 3'-flap, which appear to be the most preferable substrates for FEN-1. Serial intermediates with a 1 nt 3'-flap and 5' variable-length flaps are formed by cooperative functioning of Pyrococcus horikoshii FEN-1 with either B or D DNA polymerases Pyrococcus horikoshii OT-3 ?
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?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerases D
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Pyrococcus horikoshii
2.7.7.7 phPol D
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Pyrococcus horikoshii
3.1.99.B1 FEN-1
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Pyrococcus horikoshii
3.1.99.B1 flap endonuclease-1
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Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.99.B1 60
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assay at Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.99.B1 7.5
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assay at Pyrococcus horikoshii

General Information

EC Number General Information Comment Organism
3.1.99.B1 metabolism plays important roles with DNA polymerases in DNA replication, repair and recombination Pyrococcus horikoshii